| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 40, 25728-25733, October 2, 1998
From the Department of Biochemistry, University of Tennessee,
Memphis, Tennessee 38163
The Rho family GTPases are tightly regulated
between the active GTP-bound state and the inactive GDP-bound state in
a variety of signal transduction processes. Here the Rho family members Cdc42, Rac2, and RhoA were found to form reversible homodimers in both
the GTP- and the GDP-bound states. The homophilic interaction of Cdc42
and Rac2, but not RhoA, in the GTP-bound state, caused a significant
stimulation of the intrinsic GTPase activity, i.e. the
activated form of Cdc42 and Rac2 acts as GTPase-activating proteins
toward Cdc42-GTP or Rac2-GTP. The dimerization of the GTPases appeared
to be mediated by the carboxyl-terminal polybasic domain, and the
specific GTPase-activating effects of Cdc42 and Rac2 were also
attributed to the structural determinant(s) in the same region of the
molecules. Moreover, similar to the case of Cdc42 and Cdc42GAP
interaction, Cdc42-GDP interacted with tetrafluoroaluminate and
Cdc42-GTP
S (guanosine 5'-3-O-(thio)triphosphate) to form a transition state complex of the GTPase-activating reaction in which
the carboxyl-terminal determinant(s) of the GTPS-bound Cdc42 plays a
critical role. These results provide a rationale for the fast rate of
intrinsic GTP hydrolysis by Cdc42 and Rac and suggest that dimerization
may play a role in the negative regulation of specific Rho family
GTPases mediated by the carboxyl-terminal polybasic domain.
This article has been cited by other articles:
|
|
 |
|
  Y. Yamaguchi, K. Ota, and T. Ito A Novel Cdc42-interacting Domain of the Yeast Polarity Establishment Protein Bem1: IMPLICATIONS FOR MODULATION OF MATING PHEROMONE SIGNALING J. Biol. Chem., January 5, 2007; 282(1): 29 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Kozminski, L. Beven, E. Angerman, A. H. Y. Tong, C. Boone, and H.-O. Park Interaction between a Ras and a Rho GTPase Couples Selection of a Growth Site to the Development of Cell Polarity in Yeast Mol. Biol. Cell, December 1, 2003; 14(12): 4958 - 4970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kulkarni, D. E. Goll, and J. E. B. Fox Calpain Cleaves RhoA Generating a Dominant-negative Form That Inhibits Integrin-induced Actin Filament Assembly and Cell Spreading J. Biol. Chem., June 28, 2002; 277(27): 24435 - 24441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. van Golen, L. Bao, M. M. DiVito, Z. Wu, G. C. Prendergast, and S. D. Merajver Reversion of RhoC GTPase-induced Inflammatory Breast Cancer Phenotype by Treatment with a Farnesyl Transferase Inhibitor Mol. Cancer Ther., June 1, 2002; 1(8): 575 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Amor, J. R. Horton, X. Zhu, Y. Wang, C. Sullards, D. Ringe, X. Cheng, and R. A. Kahn Structures of Yeast ARF2 and ARL1. DISTINCT ROLES FOR THE N TERMINUS IN THE STRUCTURE AND FUNCTION OF ARF FAMILY GTPases J. Biol. Chem., November 2, 2001; 276(45): 42477 - 42484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-C. Yang, S. J. Atkinson, Y. Gu, J. B. Borneo, A. W. Roberts, Y. Zheng, J. Pennington, and D. A. Williams Rac and Cdc42 GTPases control hematopoietic stem cell shape, adhesion, migration, and mobilization PNAS, April 18, 2001; (2001) 101546898. [Abstract] [Full Text]
|
|
|
|
|
|
S. N. P. Murthy and L. Lorand Nucleotide binding by the erythrocyte transglutaminase/Gh protein, probed with fluorescent analogs of GTP and GDP PNAS, June 23, 2000; (2000) 140210197. [Abstract] [Full Text]
|
|
|
|
|
|
B. C. Low, K. T. Seow, and G. R. Guy Evidence for a Novel Cdc42GAP Domain at the Carboxyl Terminus of BNIP-2 J. Biol. Chem., May 5, 2000; 275(19): 14415 - 14422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kasper, N. Tidow, D. Grothues, and K. Welte Differential expression and regulation of GTPases (RhoA and Rac2) and GDIs (LyGDI and RhoGDI) in neutrophils from patients with severe congenital neutropenia Blood, May 1, 2000; 95(9): 2947 - 2953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Inouye, S. Mizutani, H. Koide, and Y. Kaziro Formation of the Ras Dimer Is Essential for Raf-1 Activation J. Biol. Chem., February 11, 2000; 275(6): 3737 - 3740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Low, Y. P. Lim, J. Lim, E. S. M. Wong, and G. R. Guy Tyrosine Phosphorylation of the Bcl-2-associated Protein BNIP-2 by Fibroblast Growth Factor Receptor-1 Prevents Its Binding to Cdc42GAP and Cdc42 J. Biol. Chem., November 12, 1999; 274(46): 33123 - 33130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Li, B. Debreceni, B. Jia, Y. Gao, G. Tigyi, and Y. Zheng Localization of the PAK1-, WASP-, and IQGAP1-specifying Regions of Cdc42 J. Biol. Chem., October 15, 1999; 274(42): 29648 - 29654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nakashima, E. Noguchi, and T. Nishimoto Saccharomyces cerevisiae Putative G Protein, Gtr1p, Which Forms Complexes With Itself and a Novel Protein Designated as Gtr2p, Negatively Regulates the Ran/Gsp1p G Protein Cycle Through Gtr2p Genetics, July 1, 1999; 152(3): 853 - 867. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. I. Johnson Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity Microbiol. Mol. Biol. Rev., March 1, 1999; 63(1): 54 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhang, Y. Zhang, C. C. Collins, D. I. Johnson, and Y. Zheng A Built-in Arginine Finger Triggers the Self-stimulatory GTPase-activating Activity of Rho Family GTPases J. Biol. Chem., January 29, 1999; 274(5): 2609 - 2612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhang, Y. Zhang, Z.-x. Wang, and Y. Zheng The Role of Mg2+ Cofactor in the Guanine Nucleotide Exchange and GTP Hydrolysis Reactions of Rho Family GTP-binding Proteins J. Biol. Chem., August 11, 2000; 275(33): 25299 - 25307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Low, K. T. Seow, and G. R. Guy The BNIP-2 and Cdc42GAP Homology Domain of BNIP-2 Mediates Its Homophilic Association and Heterophilic Interaction with Cdc42GAP J. Biol. Chem., November 22, 2000; 275(48): 37742 - 37751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhang, Y. Gao, S. Y. Moon, Y. Zhang, and Y. Zheng Oligomerization of Rac1 GTPase Mediated by the Carboxyl-terminal Polybasic Domain J. Biol. Chem., March 16, 2001; 276(12): 8958 - 8967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-C. Yang, S. J. Atkinson, Y. Gu, J. B. Borneo, A. W. Roberts, Y. Zheng, J. Pennington, and D. A. Williams Rac and Cdc42 GTPases control hematopoietic stem cell shape, adhesion, migration, and mobilization PNAS, May 8, 2001; 98(10): 5614 - 5618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. P. Murthy and L. Lorand Nucleotide binding by the erythrocyte transglutaminase/Gh protein, probed with fluorescent analogs of GTP and GDP PNAS, July 5, 2000; 97(14): 7744 - 7747. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
