JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kasus-Jacobi, A.
Right arrow Articles by Burnol, A.-F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kasus-Jacobi, A.
Right arrow Articles by Burnol, A.-F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 40, 26026-26035, October 2, 1998

Identification of the Rat Adapter Grb14 as an Inhibitor of Insulin Actions

Anne Kasus-JacobiDagger , Dominique PerdereauDagger , Colette Auzan, Eric Clauser, Emmanuel Van Obberghenparallel , Franck Mauvais-JarvisDagger , Jean GirardDagger , and Anne-Françoise BurnolDagger

From Dagger  Endocrinologie Métabolisme et Developpement, CNRS, UPR 1524, 9 rue Jules Hetzel, 92190 Meudon, France,  INSERM U36, Collège de France, 3 rue d'Ulm, 75005 Paris, France, and parallel  INSERM U145, Avenue de Valombrose, 06107 Nice, France

We cloned by interaction with the beta -subunit of the insulin receptor the rat variant of the human adapter Grb14 (rGrb14). rGrb14 is specifically expressed in rat insulin-sensitive tissues and in the brain. The binding of rGrb14 to insulin receptors is insulin-dependent in vivo in Chinese hamster ovary (CHO) cells overexpressing both proteins and importantly, in rat liver expressing physiological levels of proteins. However, rGrb14 is not a substrate of the tyrosine kinase of the receptor. In the two-hybrid system, two domains of rGrb14 can mediate the interaction with insulin receptors: the Src homology 2 (SH2) domain and a region between the PH and SH2 domains that we named PIR (for phosphorylated insulin receptor-interacting region). In vitro interaction assays using deletion mutants of rGrb14 show that the PIR, but not the SH2 domain, is able to coprecipitate insulin receptors, suggesting that the PIR is the major binding domain of rGrb14. The interaction between rGrb14 and the insulin receptors is almost abolished by mutating tyrosine residue Tyr1150 or Tyr1151 of the receptor. The overexpression of rGrb14 in CHO-IR cells decreases insulin stimulation of both DNA and glycogen synthesis. These effects are accompanied by a decrease in insulin-stimulated tyrosine phosphorylation of IRS-1, but insulin receptor autophosphorylation is unaltered. These findings suggest that rGrb14 could be a new downstream signaling component of the insulin-mediated pathways.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
N. Carre, M. Cauzac, J. Girard, and A.-F. Burnol
Dual Effect of the Adapter Growth Factor Receptor-Bound Protein 14 (Grb14) on Insulin Action in Primary Hepatocytes
Endocrinology, June 1, 2008; 149(6): 3109 - 3117.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
F. M. Smith, L. J. Holt, A. S. Garfield, M. Charalambous, F. Koumanov, M. Perry, R. Bazzani, S. A. Sheardown, B. D. Hegarty, R. J. Lyons, et al.
Mice with a Disruption of the Imprinted Grb10 Gene Exhibit Altered Body Composition, Glucose Homeostasis, and Insulin Signaling during Postnatal Life
Mol. Cell. Biol., August 15, 2007; 27(16): 5871 - 5886.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
K. W. Gaido, J. B. Hensley, D. Liu, D. G. Wallace, S. Borghoff, K. J. Johnson, S. J. Hall, and K. Boekelheide
Fetal Mouse Phthalate Exposure Shows that Gonocyte Multinucleation is Not Associated with Decreased Testicular Testosterone
Toxicol. Sci., June 1, 2007; 97(2): 491 - 503.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. J. Hsiao, M. F. Poitras, B. D. Cook, Y. Liu, and S. Smith
Tankyrase 2 Poly(ADP-Ribose) Polymerase Domain-Deleted Mice Exhibit Growth Defects but Have Normal Telomere Length and Capping.
Mol. Cell. Biol., March 1, 2006; 26(6): 2044 - 2054.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Liu, K. P. Lehmann, M. Sar, S. S. Young, and K. W. Gaido
Gene Expression Profiling Following In Utero Exposure to Phthalate Esters Reveals New Gene Targets in the Etiology of Testicular Dysgenesis
Biol Reprod, July 1, 2005; 73(1): 180 - 192.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. C. King and A. C. Newton
The Adaptor Protein Grb14 Regulates the Localization of 3-Phosphoinositide-dependent Kinase-1
J. Biol. Chem., September 3, 2004; 279(36): 37518 - 37527.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Deng, S. Bhattacharya, O. R. Swamy, R. Tandon, Y. Wang, R. Janda, and H. Riedel
Growth Factor Receptor-binding Protein 10 (Grb10) as a Partner of Phosphatidylinositol 3-Kinase in Metabolic Insulin Action
J. Biol. Chem., October 10, 2003; 278(41): 39311 - 39322.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. S. Lock, M. M. Frigault, C. Saucier, and M. Park
Grb2-independent Recruitment of Gab1 Requires the C-terminal Lobe and Structural Integrity of the Met Receptor Kinase Domain
J. Biol. Chem., August 8, 2003; 278(32): 30083 - 30090.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. G. Stein, R. Ghirlando, and S. R. Hubbard
Structural Basis for Dimerization of the Grb10 Src Homology 2 Domain. IMPLICATIONS FOR LIGAND SPECIFICITY
J. Biol. Chem., April 4, 2003; 278(15): 13257 - 13264.
[Abstract] [Full Text] [PDF]

Home page
 Recent Prog Horm ResHome page
J. Dupont, S. E. Dunn, J. C. Barrett, and D. LeRoith
Microarray Analysis and Identification of Novel Molecules Involved in Insulin-like Growth Factor-1 Receptor Signaling and Gene Expression
Recent Prog. Horm. Res., January 1, 2003; 58(1): 325 - 342.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Cariou, D. Perdereau, K. Cailliau, E. Browaeys-Poly, V. Bereziat, M. Vasseur-Cognet, J. Girard, and A.-F. Burnol
The Adapter Protein ZIP Binds Grb14 and Regulates Its Inhibitory Action on Insulin Signaling by Recruiting Protein Kinase C{zeta}
Mol. Cell. Biol., October 15, 2002; 22(20): 6959 - 6970.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Bereziat, A. Kasus-Jacobi, D. Perdereau, B. Cariou, J. Girard, and A.-F. Burnol
Inhibition of Insulin Receptor Catalytic Activity by the Molecular Adapter Grb14
J. Biol. Chem., February 8, 2002; 277(7): 4845 - 4852.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. F. Reilly, G. Mickey, and P. A. Maher
Association of Fibroblast Growth Factor Receptor 1 with the Adaptor Protein Grb14. CHARACTERIZATION OF A NEW RECEPTOR BINDING PARTNER
J. Biol. Chem., March 10, 2000; 275(11): 7771 - 7778.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Nantel, M. Huber, and D. Y. Thomas
Localization of Endogenous Grb10 to the Mitochondria and Its Interaction with the Mitochondrial-associated Raf-1 Pool
J. Biol. Chem., December 10, 1999; 274(50): 35719 - 35724.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Jones, Z. Master, J. Jones, D. Bouchard, Y. Gunji, H. Sasaki, R. Daly, K. Alitalo, and D. J. Dumont
Identification of Tek/Tie2 Binding Partners. BINDING TO A MULTIFUNCTIONAL DOCKING SITE MEDIATES CELL SURVIVAL AND MIGRATION
J. Biol. Chem., October 22, 1999; 274(43): 30896 - 30905.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Wang, H. Dai, N. Yousaf, M. Moussaif, Y. Deng, A. Boufelliga, O. R. Swamy, M. E. Leone, and H. Riedel
Grb10, a Positive, Stimulatory Signaling Adapter in Platelet-Derived Growth Factor BB-, Insulin-Like Growth Factor I-, and Insulin-Mediated Mitogenesis
Mol. Cell. Biol., September 1, 1999; 19(9): 6217 - 6228.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Munoz-Alonso, G. Guillemain, N. Kassis, J. Girard, A.-F. Burnol, and A. Leturque
A Novel Cytosolic Dual Specificity Phosphatase, Interacting with Glucokinase, Increases Glucose Phosphorylation Rate
J. Biol. Chem., October 13, 2000; 275(42): 32406 - 32412.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Lyons, R. Deane, D. K. Lynch, Z.-S. J. Ye, G. M. Sanderson, H. J. Eyre, G. R. Sutherland, and R. J. Daly
Identification of a Novel Human Tankyrase through Its Interaction with the Adaptor Protein Grb14
J. Biol. Chem., May 11, 2001; 276(20): 17172 - 17180.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.