| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 41, 26323-26329, October 9, 1998
From the Department of Pathology, Harvard Medical School, Beth
Israel Deaconess Medical Center, Boston, Massachusetts 02215
The vascular endothelium is uniquely positioned
between the blood and tissue compartments to receive directly the fluid
forces generated by the blood flowing through the vasculature. These forces invoke specific responses within endothelial cells and serve to
modulate their intrinsic structure and function. The mechanisms by
which hemodynamic forces are detected and converted by endothelia into
a sequence of biological and even pathological responses are presently
unknown. By purifying and subfractionating the luminal endothelial cell
plasma membrane from tissue, we show, for the first time, that not only
does mechanotransduction occur at the endothelial cell surface directly
exposed to vascular flow in vivo but also increased flow
in situ induces rapid tyrosine phosphorylation of luminal
endothelial cell surface proteins located primarily in the plasmalemmal
invaginations called caveolae. Increased flow induces the translocation
of signaling molecules primarily to caveolae, ultimately activating the
Ras-Raf-mitogen-activated protein kinase pathway. This signaling
appears to require intact caveolae. Filipin-induced disassembly of
caveolae inhibits both proximal signaling events at the cell surface
and downstream activation of the mitogen-activated protein kinase
pathway. With the molecular machinery required for mediating rapid
flow-induced responses as seen in endothelium, caveolae may be
flow-sensing organelles converting mechanical stimuli into chemical
signals transmitted into the cell.
This article has been cited by other articles:
|
|
 |
|
  V. Rizzo Lights, camera, actin! The cytoskeleton takes center stage in mechanotransduction. Focus on "Mapping the dynamics of shear stress-induced structural changes in endothelial cells." Am J Physiol Cell Physiol, December 1, 2007; 293(6): C1771 - C1772. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Albinsson, Y. Shakirova, A. Rippe, M. Baumgarten, B.-I. Rosengren, C. Rippe, R. Hallmann, P. Hellstrand, B. Rippe, and K. Sward Arterial remodeling and plasma volume expansion in caveolin-1-deficient mice Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R1222 - R1231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Peng, D. Wu, A. J. Ingram, B. Zhang, B. Gao, and J. C. Krepinsky RhoA Activation in Mesangial Cells by Mechanical Strain Depends on Caveolae and Caveolin-1 Interaction J. Am. Soc. Nephrol., January 1, 2007; 18(1): 189 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Ingber Cellular mechanotransduction: putting all the pieces together again FASEB J, May 1, 2006; 20(7): 811 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Fleming Segregation and integration: Roles played by caveolae and caveolins in the cardiovascular system Cardiovasc Res, March 1, 2006; 69(4): 784 - 787. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rubin, T. C. Murphy, J. Rahnert, H. Song, M. S. Nanes, E. M. Greenfield, H. Jo, and X. Fan Mechanical Inhibition of RANKL Expression Is Regulated by H-Ras-GTPase J. Biol. Chem., January 20, 2006; 281(3): 1412 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Bagi, J. A. Frangos, J.-C. Yeh, C. R. White, G. Kaley, and A. Koller PECAM-1 Mediates NO-Dependent Dilation of Arterioles to High Temporal Gradients of Shear Stress Arterioscler. Thromb. Vasc. Biol., August 1, 2005; 25(8): 1590 - 1595. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Radel and V. Rizzo Integrin mechanotransduction stimulates caveolin-1 phosphorylation and recruitment of Csk to mediate actin reorganization Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H936 - H945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. NAVARRO, B. ANAND-APTE, and M.-O. PARAT A role for caveolae in cell migration FASEB J, December 1, 2004; 18(15): 1801 - 1811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. O. Lungu, Z.-G. Jin, H. Yamawaki, T. Tanimoto, C. Wong, and B. C. Berk Cyclosporin A Inhibits Flow-mediated Activation of Endothelial Nitric-oxide Synthase by Altering Cholesterol Content in Caveolae J. Biol. Chem., November 19, 2004; 279(47): 48794 - 48800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Syntichaki and N. Tavernarakis Genetic Models of Mechanotransduction: The Nematode Caenorhabditis elegans Physiol Rev, October 1, 2004; 84(4): 1097 - 1153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Czarny and J. E. Schnitzer Neutral sphingomyelinase inhibitor scyphostatin prevents and ceramide mimics mechanotransduction in vascular endothelium Am J Physiol Heart Circ Physiol, September 1, 2004; 287(3): H1344 - H1352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-P. Gratton, P. Bernatchez, and W. C. Sessa Caveolae and Caveolins in the Cardiovascular System Circ. Res., June 11, 2004; 94(11): 1408 - 1417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Ferraro, M. Daneshmand, R. Bizios, and V. Rizzo Depletion of plasma membrane cholesterol dampens hydrostatic pressure and shear stress-induced mechanotransduction pathways in osteoblast cultures Am J Physiol Cell Physiol, April 1, 2004; 286(4): C831 - C839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Carver, J. E. Schnitzer, R. G. W. Anderson, and S. Mohla Role of Caveolae and Lipid Rafts in Cancer: Workshop Summary and Future Needs Cancer Res., October 15, 2003; 63(20): 6571 - 6574. [Full Text] [PDF]
|
|
|
|
|
|
V. Rizzo, C. Morton, N. DePaola, J. E. Schnitzer, and P. F. Davies Recruitment of endothelial caveolae into mechanotransduction pathways by flow conditioning in vitro Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1720 - H1729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zeidan, J. Broman, P. Hellstrand, and K. Sward Cholesterol Dependence of Vascular ERK1/2 Activation and Growth in Response to Stretch: Role of Endothelin-1 Arterioscler. Thromb. Vasc. Biol., September 1, 2003; 23(9): 1528 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Boyd, H. Park, H. Yi, Y. C. Boo, G. P. Sorescu, M. Sykes, and H. Jo Chronic shear induces caveolae formation and alters ERK and Akt responses in endothelial cells Am J Physiol Heart Circ Physiol, August 7, 2003; 285(3): H1113 - H1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ogata Increase in epidermal growth factor receptor protein induced in osteoblastic cells after exposure to flow of culture media Am J Physiol Cell Physiol, August 1, 2003; 285(2): C425 - C432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Frank, S. E. Woodman, D. S. Park, and M. P. Lisanti Caveolin, Caveolae, and Endothelial Cell Function Arterioscler. Thromb. Vasc. Biol., July 1, 2003; 23(7): 1161 - 1168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-K. Wang, Y.-H. Wang, C.-Z. Wang, J.-M. Sung, W.-T. Chiu, S.-H. Lin, Y.-H. Chang, and M.-J. Tang Rigidity of Collagen Fibrils Controls Collagen Gel-induced Down-regulation of Focal Adhesion Complex Proteins Mediated by {alpha}2{beta}1 Integrin J. Biol. Chem., June 6, 2003; 278(24): 21886 - 21892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-P. Qiu, Q. Hu, N. Paolocci, R. C. Ziegelstein, and D. A. Kass Differential effects of pulsatile versus steady flow on coronary endothelial membrane potential Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H341 - H346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. Helmke, A. B. Rosen, and P. F. Davies Mapping Mechanical Strain of an Endogenous Cytoskeletal Network in Living Endothelial Cells Biophys. J., April 1, 2003; 84(4): 2691 - 2699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. F. Davies, J. Zilberberg, and B. P. Helmke Spatial Microstimuli in Endothelial Mechanosignaling Circ. Res., March 7, 2003; 92(4): 359 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Czarny, J. Liu, P. Oh, and J. E. Schnitzer Transient Mechanoactivation of Neutral Sphingomyelinase in Caveolae to Generate Ceramide J. Biol. Chem., February 7, 2003; 278(7): 4424 - 4430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Empig and M. A. Goldsmith Association of the Caveola Vesicular System with Cellular Entry by Filoviruses J. Virol., April 16, 2002; 76(10): 5266 - 5270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. Alenghat and D. E. Ingber Mechanotransduction: All Signals Point to Cytoskeleton, Matrix, and Integrins Sci. Signal., February 12, 2002; 2002(119): pe6 - pe6. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Groschner Two ways to feel the pressure: an endothelial Ca2+ entry channel with dual mechanosensitivity Cardiovasc Res, January 1, 2002; 53(1): 9 - 11. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wei, A. B. Al-Mehdi, and A. B. Fisher Signaling pathway for nitric oxide generation with simulated ischemia in flow-adapted endothelial cells Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H2226 - H2232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Nilius and G. Droogmans Ion Channels and Their Functional Role in Vascular Endothelium Physiol Rev, October 1, 2001; 81(4): 1415 - 1459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Oh and J. E. Schnitzer Segregation of Heterotrimeric G Proteins in Cell Surface Microdomains. Gq Binds Caveolin to Concentrate in Caveolae, whereas Gi and Gs Target Lipid Rafts by Default Mol. Biol. Cell, March 1, 2001; 12(3): 685 - 698. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Govers and T. J. Rabelink Cellular regulation of endothelial nitric oxide synthase Am J Physiol Renal Physiol, February 1, 2001; 280(2): F193 - F206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. B. Babiychuk and A. Draeger Annexins in Cell Membrane Dynamics: Ca2+-regulated Association of Lipid Microdomains J. Cell Biol., September 5, 2000; 150(5): 1113 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Park, Y.-M. Go, R. Darji, J.-W. Choi, M. P. Lisanti, M. C. Maland, and H. Jo Caveolin-1 regulates shear stress-dependent activation of extracellular signal-regulated kinase Am J Physiol Heart Circ Physiol, April 1, 2000; 278(4): H1285 - H1293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J D Pearson Normal endothelial cell function Lupus, March 1, 2000; 9(3): 183 - 188. [Abstract] [PDF]
|
|
|
|
|
|
H. Rosenfeldt and F. Grinnell Fibroblast Quiescence and the Disruption of ERK Signaling in Mechanically Unloaded Collagen Matrices J. Biol. Chem., February 4, 2000; 275(5): 3088 - 3092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Go, R. P. Patel, M. C. Maland, H. Park, J. S. Beckman, V. M. Darley-Usmar, and H. Jo Evidence for peroxynitrite as a signaling molecule in flow-dependent activation of c-Jun NH2-terminal kinase Am J Physiol Heart Circ Physiol, October 1, 1999; 277(4): H1647 - H1653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Oh and J. E. Schnitzer Immunoisolation of Caveolae with High Affinity Antibody Binding to the Oligomeric Caveolin Cage. TOWARD UNDERSTANDING THE BASIS OF PURIFICATION J. Biol. Chem., August 13, 1999; 274(33): 23144 - 23154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Rizzo, D. P. McIntosh, P. Oh, and J. E. Schnitzer In Situ Flow Activates Endothelial Nitric Oxide Synthase in Luminal Caveolae of Endothelium with Rapid Caveolin Dissociation and Calmodulin Association J. Biol. Chem., December 25, 1998; 273(52): 34724 - 34729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Al-Mehdi, C. Song, K. Tozawa, and A. B. Fisher Ca2+- and Phosphatidylinositol 3-Kinase-dependent Nitric Oxide Generation in Lung Endothelial Cells in Situ with Ischemia J. Biol. Chem., December 15, 2000; 275(51): 39807 - 39810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fringer and F. Grinnell Fibroblast Quiescence in Floating or Released Collagen Matrices. CONTRIBUTION OF THE ERK SIGNALING PATHWAY AND ACTIN CYTOSKELETAL ORGANIZATION J. Biol. Chem., August 10, 2001; 276(33): 31047 - 31052. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
