HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, Z.-S. Articles by Wu, H.-N. Search for Related Content PubMed PubMed Citation Articles by Huang, Z.-S. Articles by Wu, H.-N. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 41, 26455-26461, October 9, 1998

Identification and Characterization of the RNA Chaperone Activity of Hepatitis Delta Antigen Peptides

From the Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan, Republic of China

In this study, we identified an activity of the hepatitis delta antigen that both modulates the cis-cleaving activities of hepatitis delta virus (HDV) genomic RNA fragments and facilitates the trans-cleavage reactions between hammerhead ribozymes and the cognate substrates of various lengths in vitro. Hepatitis delta antigen peptides exert their effect by accelerating the unfolding and refolding of RNA molecules and by promoting strand annealing and strand dissociation. In addition, the stimulatory effect of hepatitis delta antigen peptide on hammerhead catalysis is observed whether the peptide is removed or not by phenol/chloroform extraction prior to the initiation of trans-cleavage reaction. Therefore, hepatitis delta antigen peptide acts as an RNA chaperone. The RNA chaperone domain of hepatitis delta antigen overlaps with the coiled-coil domain that is rich in lysine residues. The RNA binding domains of hepatitis delta antigen previously identified are not required for the RNA chaperone activity identified herein. The RNA chaperone activity of hepatitis delta antigen may be important for the regulation of HDV replication in vivo.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				C.-H. Tseng, K.-S. Jeng, and M. M. C. Lai Transcription of Subgenomic mRNA of Hepatitis Delta Virus Requires a Modified Hepatitis Delta Antigen That Is Distinct from Antigenomic RNA Synthesis J. Virol., October 1, 2008; 82(19): 9409 - 9416. [Abstract] [Full Text] [PDF]

				W.-H. Huang, R.-T. Mai, and Y.-H. Wu Lee Transcription Factor YY1 and Its Associated Acetyltransferases CBP and p300 Interact with Hepatitis Delta Antigens and Modulate Hepatitis Delta Virus RNA Replication J. Virol., August 1, 2008; 82(15): 7313 - 7324. [Abstract] [Full Text] [PDF]

				D. M. Chadalavada, A. L. Cerrone-Szakal, and P. C. Bevilacqua Wild-type is the optimal sequence of the HDV ribozyme under cotranscriptional conditions RNA, December 1, 2007; 13(12): 2189 - 2201. [Abstract] [Full Text] [PDF]

				M. M. C. Lai RNA Replication without RNA-Dependent RNA Polymerase: Surprises from Hepatitis Delta Virus J. Virol., July 1, 2005; 79(13): 7951 - 7958. [Full Text] [PDF]

				M. P. ROBERTSON, S. M. KNUDSEN, and A. D. ELLINGTON In vitro selection of ribozymes dependent on peptides for activity RNA, January 1, 2004; 10(1): 114 - 127. [Abstract] [Full Text] [PDF]

				C. Zhang, H. Yan, and Z. F. Burton Combinatorial Control of Human RNA Polymerase II (RNAP II) Pausing and Transcript Cleavage by Transcription Factor IIF, Hepatitis {delta} Antigen, and Stimulatory Factor II J. Biol. Chem., December 12, 2003; 278(50): 50101 - 50111. [Abstract] [Full Text] [PDF]

				C.-C. Wang, T.-C. Chang, C.-W. Lin, H.-L. Tsui, P. B. C. Chu, B.-S. Chen, Z.-S. Huang, and H.-N. Wu Nucleic acid binding properties of the nucleic acid chaperone domain of hepatitis delta antigen Nucleic Acids Res., November 15, 2003; 31(22): 6481 - 6492. [Abstract] [Full Text] [PDF]

				Z.-S. Huang, W.-H. Su, J.-L. Wang, and H.-N. Wu Selective Strand Annealing and Selective Strand Exchange Promoted by the N-terminal Domain of Hepatitis Delta Antigen J. Biol. Chem., February 14, 2003; 278(8): 5685 - 5693. [Abstract] [Full Text] [PDF]

				M. Kameoka, M. Morgan, M. Binette, R. S. Russell, L. Rong, X. Guo, A. Mouland, L. Kleiman, C. Liang, and M. A. Wainberg The Tat Protein of Human Immunodeficiency Virus Type 1 (HIV-1) Can Promote Placement of tRNA Primer onto Viral RNA and Suppress Later DNA Polymerization in HIV-1 Reverse Transcription J. Virol., March 19, 2002; 76(8): 3637 - 3645. [Abstract] [Full Text] [PDF]

				T. B. Macnaughton, S. T. Shi, L. E. Modahl, and M. M. C. Lai Rolling Circle Replication of Hepatitis Delta Virus RNA Is Carried Out by Two Different Cellular RNA Polymerases J. Virol., March 19, 2002; 76(8): 3920 - 3927. [Abstract] [Full Text] [PDF]

				L. E. Modahl, T. B. Macnaughton, N. Zhu, D. L. Johnson, and M. M. C. Lai RNA-Dependent Replication and Transcription of Hepatitis Delta Virus RNA Involve Distinct Cellular RNA Polymerases Mol. Cell. Biol., August 15, 2000; 20(16): 6030 - 6039. [Abstract] [Full Text]

				W.-H. Huang, B. Y. M. Yung, W.-J. Syu, and Y.-H. W. Lee The Nucleolar Phosphoprotein B23 Interacts with Hepatitis Delta Antigens and Modulates the Hepatitis Delta Virus RNA Replication J. Biol. Chem., June 29, 2001; 276(27): 25166 - 25175. [Abstract] [Full Text] [PDF]