HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rhodes, K. Articles by Oshima, R. G. Search for Related Content PubMed PubMed Citation Articles by Rhodes, K. Articles by Oshima, R. G. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 41, 26534-26542, October 9, 1998

A Regulatory Element of the Human Keratin 18 Gene with AP-1-dependent Promoter Activity

From The Burnham Institute, La Jolla, California 92037

The human keratin 18 (K18) gene is expressed in a restricted but diverse subset of differentiated epithelial tissues and carcinomas. The 10-kilobase pair K18 gene contains all of the genetic information necessary for tissue-specific, copy number-dependent and integration site-independent expression in transgenic mice. We identified a 100-base pair regulatory element that activates the K18 proximal promoter in the presence of the previously identified first intron enhancer. Deletion of the element greatly diminished K18 expression. This regulatory element also has cryptic, AP-1-dependent promoter activity in the absence of the normal promoter, which results in 10-40-fold higher levels of K18 RNA expression in transgenic mice. The high activity of this cryptic promoter is dependent upon the first intron enhancer. These experiments define interactive regulatory regions of the K18 gene that modulate expression in diverse epithelial cell types and identify an unusual regulatory element with promoter activity that may be useful for high level heterologous gene expression in transgenic animals.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				T. E. Uveges, Y. Shan, B. E. Kramer, D. C. Wight, and L. M. Parysek Intron 1 Is Required for Cell Type-Specific, But Not Injury-Responsive, Peripherin Gene Expression J. Neurosci., September 15, 2002; 22(18): 7959 - 7967. [Abstract] [Full Text] [PDF]

				S. E. Rutberg, T. L. Adams, A. Glick, M. T. Bonovich, C. Vinson, and S. H. Yuspa Activator Protein 1 Transcription Factors Are Fundamental to v-rasHa-induced Changes in Gene Expression in Neoplastic Keratinocytes Cancer Res., November 1, 2000; 60(22): 6332 - 6338. [Abstract] [Full Text]

				D. A. Willoughby, A. Vilalta, and R. G. Oshima An Alu Element from the K18 Gene Confers Position-independent Expression in Transgenic Mice J. Biol. Chem., January 14, 2000; 275(2): 759 - 768. [Abstract] [Full Text] [PDF]