HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oelkers, P. Articles by Sturley, S. L. Search for Related Content PubMed PubMed Citation Articles by Oelkers, P. Articles by Sturley, S. L. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 41, 26765-26771, October 9, 1998

Characterization of Two Human Genes Encoding Acyl Coenzyme A:Cholesterol Acyltransferase-related Enzymes

Peter Oelkers, Ajay Behari, Debra Cromley^¶, Jeffrey T. Billheimer^¶, and Stephen L. Sturley

From the  Institute of Human Nutrition,  Departments of Pediatrics and Physiology and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York 10032 and ^¶ DuPont Pharmaceutical Company, Experimental Station, Wilmington, Delaware 19880-0400

The enzyme acyl coenzyme A:cholesterol acyltransferase 1 (ACAT1) mediates sterol esterification, a crucial component of intracellular lipid homeostasis. Two enzymes catalyze this activity in Saccharomyces cerevisiae (yeast), and several lines of evidence suggest multigene families may also exist in mammals. Using the human ACAT1 sequence to screen data bases of expressed sequence tags, we identified two novel and distinct partial human cDNAs. Full-length cDNA clones for these ACAT related gene products (ARGP) 1 and 2 were isolated from a hepatocyte (HepG2) cDNA library. ARGP1 was expressed in numerous human adult tissues and tissue culture cell lines, whereas expression of ARGP2 was more restricted. In vitro microsomal assays in a yeast strain deleted for both esterification genes and completely deficient in sterol esterification indicated that ARGP2 esterified cholesterol while ARGP1 did not. In contrast to ACAT1 and similar to liver esterification, the activity of ARGP2 was relatively resistant to a histidine active site modifier. ARGP2 is therefore a tissue-specific sterol esterification enzyme which we thus designated ACAT2. We speculate that ARGP1 participates in the coenzyme A-dependent acylation of substrate(s) other than cholesterol. Consistent with this hypothesis, ARGP1, unlike any other member of this multigene family, possesses a predicted diacylglycerol binding motif suggesting that it may perform the last acylation in triglyceride biosynthesis.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				T.-Y. Chang, B.-L. Li, C. C. Y. Chang, and Y. Urano Acyl-coenzyme A:cholesterol acyltransferases Am J Physiol Endocrinol Metab, July 1, 2009; 297(1): E1 - E9. [Abstract] [Full Text] [PDF]

				A. R. Turkish and S. L. Sturley The genetics of neutral lipid biosynthesis: an evolutionary perspective Am J Physiol Endocrinol Metab, July 1, 2009; 297(1): E19 - E27. [Abstract] [Full Text] [PDF]

				Y. Shi and D. Cheng Beyond triglyceride synthesis: the dynamic functional roles of MGAT and DGAT enzymes in energy metabolism Am J Physiol Endocrinol Metab, July 1, 2009; 297(1): E10 - E18. [Abstract] [Full Text] [PDF]

				L. Lei, Y. Xiong, J. Chen, J.-B. Yang, Y. Wang, X.-Y. Yang, C. C. Y. Chang, B.-L. Song, T.-Y. Chang, and B.-L. Li TNF-alpha stimulates the ACAT1 expression in differentiating monocytes to promote the CE-laden cell formation J. Lipid Res., June 1, 2009; 50(6): 1057 - 1067. [Abstract] [Full Text] [PDF]

				J. Iqbal and M. M. Hussain Intestinal lipid absorption Am J Physiol Endocrinol Metab, June 1, 2009; 296(6): E1183 - E1194. [Abstract] [Full Text] [PDF]

				C.-L. E. Yen, S. J. Stone, S. Koliwad, C. Harris, and R. V. Farese Jr. Thematic Review Series: Glycerolipids. DGAT enzymes and triacylglycerol biosynthesis J. Lipid Res., November 1, 2008; 49(11): 2283 - 2301. [Abstract] [Full Text] [PDF]

				M. A. Gijon, W. R. Riekhof, S. Zarini, R. C. Murphy, and D. R. Voelker Lysophospholipid Acyltransferases and Arachidonate Recycling in Human Neutrophils J. Biol. Chem., October 31, 2008; 283(44): 30235 - 30245. [Abstract] [Full Text] [PDF]

				J. Iqbal, L. L. Rudel, and M. M. Hussain Microsomal Triglyceride Transfer Protein Enhances Cellular Cholesteryl Esterification by Relieving Product Inhibition J. Biol. Chem., July 18, 2008; 283(29): 19967 - 19980. [Abstract] [Full Text] [PDF]

				Y. Fujiwara, N. Kiyota, M. Hori, S. Matsushita, Y. Iijima, K. Aoki, D. Shibata, M. Takeya, T. Ikeda, T. Nohara, et al. Esculeogenin A, a New Tomato Sapogenol, Ameliorates Hyperlipidemia and Atherosclerosis in ApoE-Deficient Mice by Inhibiting ACAT Arterioscler. Thromb. Vasc. Biol., November 1, 2007; 27(11): 2400 - 2406. [Abstract] [Full Text] [PDF]

				Q. Chen, L. Steinhauer, J. Hammerlindl, W. Keller, and J. Zou Biosynthesis of Phytosterol Esters: Identification of a Sterol O-Acyltransferase in Arabidopsis Plant Physiology, November 1, 2007; 145(3): 974 - 984. [Abstract] [Full Text] [PDF]

				T. A. Bell III, M. D. Wilson, K. Kelley, J. K. Sawyer, and L. L. Rudel Monounsaturated fatty acyl-coenzyme A is predictive of atherosclerosis in human apoB-100 transgenic, LDLr-/- mice J. Lipid Res., May 1, 2007; 48(5): 1122 - 1131. [Abstract] [Full Text] [PDF]

				A. Turkish and S. L. Sturley Regulation of Triglyceride Metabolism. I. Eukaryotic neutral lipid synthesis: "Many ways to skin ACAT or a DGAT" Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G953 - G957. [Abstract] [Full Text] [PDF]

				J. M. Shockey, S. K. Gidda, D. C. Chapital, J.-C. Kuan, P. K. Dhanoa, J. M. Bland, S. J. Rothstein, R. T. Mullen, and J. M. Dyer Tung Tree DGAT1 and DGAT2 Have Nonredundant Functions in Triacylglycerol Biosynthesis and Are Localized to Different Subdomains of the Endoplasmic Reticulum PLANT CELL, September 1, 2006; 18(9): 2294 - 2313. [Abstract] [Full Text] [PDF]

				R. V. Farese Jr The nine lives of ACAT inhibitors. Arterioscler. Thromb. Vasc. Biol., August 1, 2006; 26(8): 1684 - 1686. [Full Text] [PDF]

				N. Chen, L. Liu, Y. Zhang, H. N. Ginsberg, and Y.-H. Yu Whole-body Insulin Resistance in the Absence of Obesity in FVB Mice With Overexpression of Dgat1 in Adipose Tissue Diabetes, December 1, 2005; 54(12): 3379 - 3386. [Abstract] [Full Text] [PDF]

				C.-L. E. Yen, M. Monetti, B. J. Burri, and R. V. Farese Jr. The triacylglycerol synthesis enzyme DGAT1 also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters J. Lipid Res., July 1, 2005; 46(7): 1502 - 1511. [Abstract] [Full Text] [PDF]

				T. Yamazaki, E. Sasaki, C. Kakinuma, T. Yano, S. Miura, and O. Ezaki Increased Very Low Density Lipoprotein Secretion and Gonadal Fat Mass in Mice Overexpressing Liver DGAT1 J. Biol. Chem., June 3, 2005; 280(22): 21506 - 21514. [Abstract] [Full Text] [PDF]

				L. L. Rudel, R. G. Lee, and P. Parini ACAT2 Is a Target for Treatment of Coronary Heart Disease Associated With Hypercholesterolemia Arterioscler. Thromb. Vasc. Biol., June 1, 2005; 25(6): 1112 - 1118. [Abstract] [Full Text] [PDF]

				Y. R. Su, D. E. Dove, A. S. Major, A. H. Hasty, B. Boone, M. F. Linton, and S. Fazio Reduced ABCA1-Mediated Cholesterol Efflux and Accelerated Atherosclerosis in Apolipoprotein E-Deficient Mice Lacking Macrophage-Derived ACAT1 Circulation, May 10, 2005; 111(18): 2373 - 2381. [Abstract] [Full Text] [PDF]

				A. R. Turkish, A. L. Henneberry, D. Cromley, M. Padamsee, P. Oelkers, H. Bazzi, A. M. Christiano, J. T. Billheimer, and S. L. Sturley Identification of Two Novel Human Acyl-CoA Wax Alcohol Acyltransferases: MEMBERS OF THE DIACYLGLYCEROL ACYLTRANSFERASE 2 (DGAT2) GENE SUPERFAMILY J. Biol. Chem., April 15, 2005; 280(15): 14755 - 14764. [Abstract] [Full Text] [PDF]

				H. C. Chen and R. V. Farese Jr Inhibition of Triglyceride Synthesis as a Treatment Strategy for Obesity: Lessons From DGAT1-Deficient Mice Arterioscler. Thromb. Vasc. Biol., March 1, 2005; 25(3): 482 - 486. [Abstract] [Full Text] [PDF]

				K. Liang, C. H. Kim, and N. D. Vaziri HMG-CoA reductase inhibition reverses LCAT and LDL receptor deficiencies and improves HDL in rats with chronic renal failure Am J Physiol Renal Physiol, March 1, 2005; 288(3): F539 - F544. [Abstract] [Full Text] [PDF]

				J. X. Rong, J. Kusunoki, P. Oelkers, S. L. Sturley, and E. A. Fisher Acyl-CoenzymeA (CoA):Cholesterol Acyltransferase Inhibition in Rat and Human Aortic Smooth Muscle Cells Is Nontoxic and Retards Foam Cell Formation Arterioscler. Thromb. Vasc. Biol., January 1, 2005; 25(1): 122 - 127. [Abstract] [Full Text] [PDF]

				J.-Y. Lee and T. P. Carr Dietary Fatty Acids Regulate Acyl-CoA:Cholesterol Acyltransferase and Cytosolic Cholesteryl Ester Hydrolase in Hamsters J. Nutr., December 1, 2004; 134(12): 3239 - 3244. [Abstract] [Full Text] [PDF]

				R. G. Lee, K. L. Kelley, J. K. Sawyer, R. V. Farese Jr, J. S. Parks, and L. L. Rudel Plasma Cholesteryl Esters Provided by Lecithin:Cholesterol Acyltransferase and Acyl-Coenzyme A:Cholesterol Acyltransferase 2 Have Opposite Atherosclerotic Potential Circ. Res., November 12, 2004; 95(10): 998 - 1004. [Abstract] [Full Text] [PDF]

				N. D. Vaziri and K. Liang ACAT inhibition reverses LCAT deficiency and improves plasma HDL in chronic renal failure Am J Physiol Renal Physiol, November 1, 2004; 287(5): F1038 - F1043. [Abstract] [Full Text] [PDF]

				L. Yang, O. Lee, J. Chen, J. Chen, C. C. Y. Chang, P. Zhou, Z.-Z. Wang, H.-H. Ma, H.-F. Sha, J.-X. Feng, et al. Human Acyl-Coenzyme A:Cholesterol Acyltransferase 1 (acat1) Sequences Located in Two Different Chromosomes (7 and 1) Are Required to Produce a Novel ACAT1 Isoenzyme with Additional Sequence at the N Terminus J. Biol. Chem., October 29, 2004; 279(44): 46253 - 46262. [Abstract] [Full Text] [PDF]

				J. J. Liang, P. Oelkers, C. Guo, P.-C. Chu, J. L. Dixon, H. N. Ginsberg, and S. L. Sturley Overexpression of Human Diacylglycerol Acyltransferase 1, Acyl-CoA:Cholesterol Acyltransferase 1, or Acyl-CoA:Cholesterol Acyltransferase 2 Stimulates Secretion of Apolipoprotein B-containing Lipoproteins in McA-RH7777 Cells J. Biol. Chem., October 22, 2004; 279(43): 44938 - 44944. [Abstract] [Full Text] [PDF]

				P. Parini, M. Davis, A. T. Lada, S. K. Erickson, T. L. Wright, U. Gustafsson, S. Sahlin, C. Einarsson, M. Eriksson, B. Angelin, et al. ACAT2 Is Localized to Hepatocytes and Is the Major Cholesterol-Esterifying Enzyme in Human Liver Circulation, October 5, 2004; 110(14): 2017 - 2023. [Abstract] [Full Text] [PDF]

				S. H. Ganji, S. Tavintharan, D. Zhu, Y. Xing, V. S. Kamanna, and M. L. Kashyap Niacin noncompetitively inhibits DGAT2 but not DGAT1 activity in HepG2 cells J. Lipid Res., October 1, 2004; 45(10): 1835 - 1845. [Abstract] [Full Text] [PDF]

				M. Hori, M. Satoh, K. Furukawa, Y.-i. Sakamoto, H. Hakamata, Y. Komohara, M. Takeya, Y. Sasaki, A. Miyazaki, and S. Horiuchi Acyl-Coenzyme A:Cholesterol Acyltransferase-2 (ACAT-2) Is Responsible for Elevated Intestinal ACAT Activity in Diabetic Rats Arterioscler. Thromb. Vasc. Biol., September 1, 2004; 24(9): 1689 - 1695. [Abstract] [Full Text] [PDF]

				N.D. Vaziri and K.H. Liang Acyl-Coenzyme A:Cholesterol Acyltransferase Inhibition Ameliorates Proteinuria, Hyperlipidemia, Lecithin-Cholesterol Acyltransferase, SRB-1, and Low-Denisty Lipoprotein Receptor Deficiencies in Nephrotic Syndrome Circulation, July 27, 2004; 110(4): 419 - 425. [Abstract] [Full Text] [PDF]

				J. L. Smith, K. Rangaraj, R. Simpson, D. J. Maclean, L. K. Nathanson, K. A. Stuart, S. P. Scott, G. A. Ramm, and J. de Jersey Quantitative analysis of the expression of ACAT genes in human tissues by real-time PCR2 J. Lipid Res., April 1, 2004; 45(4): 686 - 696. [Abstract] [Full Text] [PDF]

				N. M. Q. Palacpac, Y. Hiramine, F. Mi-ichi, M. Torii, K. Kita, R. Hiramatsu, T. Horii, and T. Mitamura Developmental-stage-specific triacylglycerol biosynthesis, degradation and trafficking as lipid bodies in Plasmodium falciparum-infected erythrocytes J. Cell Sci., March 15, 2004; 117(8): 1469 - 1480. [Abstract] [Full Text] [PDF]

				A. T. Lada, M. Davis, C. Kent, J. Chapman, H. Tomoda, S. Omura, and L. L. Rudel Identification of ACAT1- and ACAT2-specific inhibitors using a novel, cell-based fluorescence assay: individual ACAT uniqueness J. Lipid Res., February 1, 2004; 45(2): 378 - 386. [Abstract] [Full Text] [PDF]

				I. Namatame, H. Tomoda, S. Ishibashi, and S. Omura Antiatherogenic activity of fungal beauveriolides, inhibitors of lipid droplet accumulation in macrophages PNAS, January 20, 2004; 101(3): 737 - 742. [Abstract] [Full Text] [PDF]

				Q. Zhang, H. K. Chieu, C. P. Low, S. Zhang, C. K. Heng, and H. Yang Schizosaccharomyces pombe Cells Deficient in Triacylglycerols Synthesis Undergo Apoptosis upon Entry into the Stationary Phase J. Biol. Chem., November 21, 2003; 278(47): 47145 - 47155. [Abstract] [Full Text] [PDF]

				B. Schmitt, M. Fluck, J. Decombaz, R. Kreis, C. Boesch, M. Wittwer, F. Graber, M. Vogt, H. Howald, and H. Hoppeler Transcriptional adaptations of lipid metabolism in tibialis anterior muscle of endurance-trained athletes Physiol Genomics, October 17, 2003; 15(2): 148 - 157. [Abstract] [Full Text] [PDF]

				P. Nawabi, A. Lykidis, D. Ji, and K. Haldar Neutral-Lipid Analysis Reveals Elevation of Acylglycerols and Lack of Cholesterol Esters in Plasmodium falciparum-Infected Erythrocytes Eukaryot. Cell, October 1, 2003; 2(5): 1128 - 1131. [Abstract] [Full Text] [PDF]

				S. Lin, X. Lu, C. C.Y. Chang, and T.-Y. Chang Human Acyl-Coenzyme A:Cholesterol Acyltransferase Expressed in Chinese Hamster Ovary Cells: Membrane Topology and Active Site Location Mol. Biol. Cell, June 1, 2003; 14(6): 2447 - 2460. [Abstract] [Full Text] [PDF]

				E. L. Willner, B. Tow, K. K. Buhman, M. Wilson, D. A. Sanan, L. L. Rudel, and R. V. Farese Jr. Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice PNAS, February 4, 2003; 100(3): 1262 - 1267. [Abstract] [Full Text] [PDF]

				H. Chao, M. Zhou, A. McIntosh, F. Schroeder, and A. B. Kier ACBP and cholesterol differentially alter fatty acyl CoA utilization by microsomal ACAT J. Lipid Res., January 1, 2003; 44(1): 72 - 83. [Abstract] [Full Text] [PDF]

				Y.-H. Yu, Y. Zhang, P. Oelkers, S. L. Sturley, D. J. Rader, and H. N. Ginsberg Posttranscriptional Control of the Expression and Function of Diacylglycerol Acyltransferase-1 in Mouse Adipocytes J. Biol. Chem., December 20, 2002; 277(52): 50876 - 50884. [Abstract] [Full Text] [PDF]

				K. Liang and N. D. Vaziri Upregulation of acyl-CoA:cholesterol acyltransferase in chronic renal failure Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E676 - E681. [Abstract] [Full Text] [PDF]

				C. Xie, L. A. Woollett, S. D. Turley, and J. M. Dietschy Fatty acids differentially regulate hepatic cholesteryl ester formation and incorporation into lipoproteins in the liver of the mouse J. Lipid Res., September 1, 2002; 43(9): 1508 - 1519. [Abstract] [Full Text] [PDF]

				N. M. Borradaile, L. E. de Dreu, P. H. R. Barrett, and M. W. Huff Inhibition of hepatocyte apoB secretion by naringenin: enhanced rapid intracellular degradation independent of reduced microsomal cholesteryl esters J. Lipid Res., September 1, 2002; 43(9): 1544 - 1554. [Abstract] [Full Text] [PDF]

				M. Buszczak, X. Lu, W. A. Segraves, T. Y. Chang, and L. Cooley Mutations in the midway Gene Disrupt a Drosophila Acyl Coenzyme A: Diacylglycerol Acyltransferase Genetics, April 1, 2002; 160(4): 1511 - 1518. [Abstract] [Full Text] [PDF]

				P. Oelkers, D. Cromley, M. Padamsee, J. T. Billheimer, and S. L. Sturley The DGA1 Gene Determines a Second Triglyceride Synthetic Pathway in Yeast J. Biol. Chem., March 8, 2002; 277(11): 8877 - 8881. [Abstract] [Full Text] [PDF]

				T. Y. Chang, C. C. Y. Chang, X. Lu, and S. Lin Catalysis of ACAT may be completed within the plane of the membrane: a working hypothesis J. Lipid Res., December 1, 2001; 42(12): 1933 - 1938. [Abstract] [Full Text] [PDF]

				S. Cases, S. J. Stone, P. Zhou, E. Yen, B. Tow, K. D. Lardizabal, T. Voelker, and R. V. Farese Jr. Cloning of DGAT2, a Second Mammalian Diacylglycerol Acyltransferase, and Related Family Members J. Biol. Chem., October 12, 2001; 276(42): 38870 - 38876. [Abstract] [Full Text] [PDF]

				K. Jensen-Pergakes, Z. Guo, M. Giattina, S. L. Sturley, and M. Bard Transcriptional Regulation of the Two Sterol Esterification Genes in the Yeast Saccharomyces cerevisiae J. Bacteriol., September 1, 2001; 183(17): 4950 - 4957. [Abstract] [Full Text] [PDF]

				Z. Guo, D. Cromley, J. T. Billheimer, and S. L. Sturley Identification of potential substrate-binding sites in yeast and human acyl-CoA sterol acyltransferases by mutagenesis of conserved sequences J. Lipid Res., August 1, 2001; 42(8): 1282 - 1291. [Abstract] [Full Text] [PDF]

				C. Jako, A. Kumar, Y. Wei, J. Zou, D. L. Barton, E. M. Giblin, P. S. Covello, and D. C. Taylor Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight Plant Physiology, June 1, 2001; 126(2): 861 - 874. [Abstract] [Full Text] [PDF]

				L. J. Wilcox, N. M. Borradaile, L. E. de Dreu, and M. W. Huff Secretion of hepatocyte apoB is inhibited by the flavonoids, naringenin and hesperetin, via reduced activity and expression of ACAT2 and MTP J. Lipid Res., May 1, 2001; 42(5): 725 - 734. [Abstract] [Full Text]

				K. Aragane, K. Fujinami, K. Kojima, and J. Kusunoki ACAT inhibitor F-1394 prevents intimal hyperplasia induced by balloon injury in rabbits J. Lipid Res., April 1, 2001; 42(4): 480 - 488. [Abstract] [Full Text]

				K. K. Maung, A. Miyazaki, H. Nomiyama, C. C. Y. Chang, T.-Y. Chang, and S. Horiuchi Induction of acyl-coenzyme A:cholesterol acyltransferase-1 by 1,25-dihydroxyvitamin D3 or 9-cis-retinoic acid in undifferentiated THP-1 cells J. Lipid Res., February 1, 2001; 42(2): 181 - 187. [Abstract] [Full Text]

				A. Ruiz, M. H. Kuehn, J. L. Andorf, E. Stone, G. S. Hageman, and D. Bok Genomic Organization and Mutation Analysis of the Gene Encoding Lecithin Retinol Acyltransferase in Human Retinal Pigment Epithelium Invest. Ophthalmol. Vis. Sci., January 1, 2001; 42(1): 31 - 37. [Abstract] [Full Text]

				R. G. Lee, M. C. Willingham, M. A. Davis, K. A. Skinner, and L. L. Rudel Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates J. Lipid Res., December 1, 2000; 41(12): 1991 - 2001. [Abstract] [Full Text]

				C. W. Joyce, G. S. Shelness, M. A. Davis, R. G. Lee, K. Skinner, R. A. Anderson, and L. L. Rudel ACAT1 and ACAT2 Membrane Topology Segregates a Serine Residue Essential for Activity to Opposite Sides of the Endoplasmic Reticulum Membrane Mol. Biol. Cell, November 1, 2000; 11(11): 3675 - 3687. [Abstract] [Full Text]

				N. N. Izzat, M. E. Deshazer, and D. S. Loose-Mitchell New Molecular Targets for Cholesterol-Lowering Therapy J. Pharmacol. Exp. Ther., May 1, 2000; 293(2): 315 - 320. [Abstract] [Full Text]

				J. Kusunoki, K. Aragane, T. Kitamine, H. Kozono, K. Kano, K. Fujinami, K. Kojima, T. Chiwata, and Y. Sekine Postprandial Hyperlipidemia in Streptozotocin-Induced Diabetic Rats Is Due to Abnormal Increase in Intestinal Acyl Coenzyme A:Cholesterol Acyltransferase Activity Arterioscler. Thromb. Vasc. Biol., January 1, 2000; 20(1): 171 - 178. [Abstract] [Full Text] [PDF]

				N. Sakashita, A. Miyazaki, M. Takeya, S. Horiuchi, C. C. Y. Chang, T.-Y. Chang, and K. Takahashi Localization of Human Acyl-Coenzyme A:Cholesterol Acyltransferase-1 (ACAT-1) in Macrophages and in Various Tissues Am. J. Pathol., January 1, 2000; 156(1): 227 - 236. [Abstract] [Full Text] [PDF]

				C. Yu, J. Chen, S. Lin, J. Liu, C. C. Y. Chang, and T.-Y. Chang Human Acyl-CoA:Cholesterol Acyltransferase-1 Is a Homotetrameric Enzyme in Intact Cells and in Vitro J. Biol. Chem., December 17, 1999; 274(51): 36139 - 36145. [Abstract] [Full Text] [PDF]

				K. A. H. Abo-Hashema, M. H. Cake, G. W. Power, and D. Clarke Evidence for Triacylglycerol Synthesis in the Lumen of Microsomes via a Lipolysis-Esterification Pathway Involving Carnitine Acyltransferases J. Biol. Chem., December 10, 1999; 274(50): 35577 - 35582. [Abstract] [Full Text] [PDF]

				S. Lin, D. Cheng, M.-S. Liu, J. Chen, and T.-Y. Chang Human Acyl-CoA:Cholesterol Acyltransferase-1 in the Endoplasmic Reticulum Contains Seven Transmembrane Domains J. Biol. Chem., August 13, 1999; 274(33): 23276 - 23285. [Abstract] [Full Text] [PDF]

				J. R. Burnett, L. J. Wilcox, D. E. Telford, S. J. Kleinstiver, P. H. R. Barrett, R. S. Newton, and M. W. Huff Inhibition of ACAT by avasimibe decreases both VLDL and LDL apolipoprotein B production in miniature pigs J. Lipid Res., July 1, 1999; 40(7): 1317 - 1328. [Abstract] [Full Text]

				R. L. Hamilton, A. Moorehouse, S. R. Lear, J. S. Wong, and S. K. Erickson A rapid calcium precipitation method of recovering large amounts of highly pure hepatocyte rough endoplasmic reticulum J. Lipid Res., June 1, 1999; 40(6): 1140 - 1147. [Abstract] [Full Text]

				D. Warnecke, R. Erdmann, A. Fahl, B. Hube, F. Muller, T. Zank, U. Zahringer, and E. Heinz Cloning and Functional Expression of UGT Genes Encoding Sterol Glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum J. Biol. Chem., May 7, 1999; 274(19): 13048 - 13059. [Abstract] [Full Text] [PDF]

				B.-L. Li, X.-L. Li, Z.-J. Duan, O. Lee, S. Lin, Z.-M. Ma, C. C. Y. Chang, X.-Y. Yang, J. P. Park, T. K. Mohandas, et al. Human Acyl-CoA:Cholesterol Acyltransferase-1 (ACAT-1) Gene Organization and Evidence That the 4.3-Kilobase ACAT-1 mRNA Is Produced from Two Different Chromosomes J. Biol. Chem., April 16, 1999; 274(16): 11060 - 11071. [Abstract] [Full Text] [PDF]

				S. Cases, S. Novak, Y.-W. Zheng, H. M. Myers, S. R. Lear, E. Sande, C. B. Welch, A. J. Lusis, T. A. Spencer, B. R. Krause, et al. ACAT-2, A Second Mammalian Acyl-CoA:Cholesterol Acyltransferase. ITS CLONING, EXPRESSION, AND CHARACTERIZATION J. Biol. Chem., October 9, 1998; 273(41): 26755 - 26764. [Abstract] [Full Text] [PDF]

				P. Oelkers, A. Tinkelenberg, N. Erdeniz, D. Cromley, J. T. Billheimer, and S. L. Sturley A Lecithin Cholesterol Acyltransferase-like Gene Mediates Diacylglycerol Esterification in Yeast J. Biol. Chem., May 19, 2000; 275(21): 15609 - 15612. [Abstract] [Full Text] [PDF]

				H. Yagyu, T. Kitamine, J.-i. Osuga, R.-i. Tozawa, Z. Chen, Y. Kaji, T. Oka, S. Perrey, Y. Tamura, K. Ohashi, et al. Absence of ACAT-1 Attenuates Atherosclerosis but Causes Dry Eye and Cutaneous Xanthomatosis in Mice with Congenital Hyperlipidemia J. Biol. Chem., July 7, 2000; 275(28): 21324 - 21330. [Abstract] [Full Text] [PDF]

				C. C. Y. Chang, N. Sakashita, K. Ornvold, O. Lee, E. T. Chang, R. Dong, S. Lin, C.-Y. G. Lee, S. C. Strom, R. Kashyap, et al. Immunological Quantitation and Localization of ACAT-1 and ACAT-2 in Human Liver and Small Intestine J. Biol. Chem., September 1, 2000; 275(36): 28083 - 28092. [Abstract] [Full Text] [PDF]

				J.-B. Yang, Z.-J. Duan, W. Yao, O. Lee, L. Yang, X.-Y. Yang, X. Sun, C. C. Y. Chang, T.-Y. Chang, and B.-L. Li Synergistic Transcriptional Activation of Human Acyl-coenzyme A: Cholesterol Acyltransterase-1 Gene by Interferon-gamma and All-trans-Retinoic Acid THP-1 Cells J. Biol. Chem., June 8, 2001; 276(24): 20989 - 20998. [Abstract] [Full Text] [PDF]

				K. K. Buhman, H. C. Chen, and R. V. Farese Jr. The Enzymes of Neutral Lipid Synthesis J. Biol. Chem., October 26, 2001; 276(44): 40369 - 40372. [Full Text] [PDF]