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J Biol Chem, Vol. 273, Issue 42, 27236-27244, October 16, 1998

GroEL and GroES Control of Substrate Flux in the in Vivo Folding Pathway of Phage P22 Coat Protein

From the Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269-3125

Our present understanding of the action of the chaperonins GroEL/S on protein folding is based primarily on in vitro studies, whereas the folding of proteins in the cellular milieu has not been as thoroughly investigated. We have developed a means of examining in vivo protein folding and assembly that utilizes the coat protein of bacteriophage P22, a naturally occurring substrate of GroEL/S. Here we show that amino acid substitutions in coat protein that cause a temperature-sensitive-folding (tsf) phenotype slowed assembly rates upon increasing the temperature of cell growth. Raising cellular concentrations of GroEL/S increased the rate of assembly of the tsf mutant coat proteins to nearly that of wild-type (WT) coat protein by protecting a thermolabile folding intermediate from aggregation, thereby increasing the concentration of assembly-competent coat protein. The rate of release of the tsf coat proteins from the GroEL/S-coat protein ternary complex was approximately 2-fold slower at non-permissive temperatures when compared with the release of WT coat protein. However, the rate of release of WT or tsf coat proteins at each temperature remained constant regardless of GroEL/S levels. Thus, raising the cellular concentration of GroEL/S increased the amount of assembly-competent tsf coat proteins not by altering the rates of folding but by increasing the probability of GroEL/S-coat protein complex formation.

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