HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shi, D. Articles by Kellems, R. E. Search for Related Content PubMed PubMed Citation Articles by Shi, D. Articles by Kellems, R. E. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 42, 27331-27338, October 16, 1998

Transcription Factor AP-2 Regulates Murine Adenosine Deaminase Gene Expression during Placental Development

Daqing Shi and Rodney E. Kellems^¶

From the  Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030 and ^¶ Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, Texas 77030

Trophoblast cells are specialized extra-embryonic cells present only in eutherian mammals. They play a major role in the implantation and placentation processes. To understand better the molecular mechanisms that control the development and function of trophoblast cells, we sought to identify the transcription factors that regulate murine adenosine deaminase (ADA) gene expression in the placenta. Here we report a detailed characterization of a placenta-specific footprinting region (FP1) in the Ada placental regulatory element. The sequence of FP1 was mapped by DNase I footprinting and was found to match a consensus AP-2 transcription factor-binding site. Electrophoretic mobility shift assays demonstrated that FP1 interacted with AP-2-like proteins. Further analysis using AP-2 antibody confirmed that AP-2 protein was indeed present in the placenta and bound to FP1. Mutation at the AP-2 site in FP1 abolished the ability of the Ada placental regulatory element to bind AP-2 proteins and failed to target chloramphenicol acetyltransferase reporter gene expression to placentas in transgenic mice, indicating that AP-2 is required for Ada expression in the placenta. In addition, RNase protection assays demonstrated that AP-2 was the predominant AP-2 family member expressed in the placenta. In situ hybridization analysis revealed that AP-2 expression was enriched in the trophoblast lineage throughout development, suggesting that AP-2 may be critical for trophoblast development and differentiation.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				N. Sher, N. Yivgi-Ohana, and J. Orly Transcriptional Regulation of the Cholesterol Side Chain Cleavage Cytochrome P450 Gene (CYP11A1) Revisited: Binding of GATA, Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein and Activating Protein (AP)-1 Proteins to a Distal Novel Cluster of cis-Regulatory Elements Potentiates AP-2 and Steroidogenic Factor-1-Dependent Gene Expression in the Rodent Placenta and Ovary Mol. Endocrinol., April 1, 2007; 21(4): 948 - 962. [Abstract] [Full Text] [PDF]

				A. Ozturk, L. J. Donald, L. Li, H. W. Duckworth, and M. L. Duckworth Proteomic Identification of AP2{gamma} as a Rat Placental Lactogen II Trophoblast Cell-Specific Enhancer Binding Protein Endocrinology, September 1, 2006; 147(9): 4319 - 4329. [Abstract] [Full Text] [PDF]

				A H K El-Hashash and S J Kimber Trophoblast differentiation in vitro: establishment and characterisation of a serum-free culture model for murine secondary trophoblast giant cells Reproduction, July 1, 2004; 128(1): 53 - 71. [Abstract] [Full Text] [PDF]

				Y.-H. Cheng, B. J. Aronow, S. Hossain, B. Trapnell, S. Kong, and S. Handwerger Critical role for transcription factor AP-2{alpha} in human trophoblast differentiation Physiol Genomics, June 17, 2004; 18(1): 99 - 107. [Abstract] [Full Text] [PDF]

				S. W. Limesand, K. M. Jeckel, and R. V. Anthony Pur{alpha}, a Single-Stranded Deoxyribonucleic Acid Binding Protein, Augments Placental Lactogen Gene Transcription Mol. Endocrinol., February 1, 2004; 18(2): 447 - 457. [Abstract] [Full Text] [PDF]

				T. A. Rodriguez, D. B. Sparrow, A. N. Scott, S. L. Withington, J. I. Preis, J. Michalicek, M. Clements, T. E. Tsang, T. Shioda, R. S. P. Beddington, et al. Cited1 Is Required in Trophoblasts for Placental Development and for Embryo Growth and Survival Mol. Cell. Biol., January 1, 2004; 24(1): 228 - 244. [Abstract] [Full Text] [PDF]

				M. Li and R. E. Kellems Sp1 and Sp3 Are Important Regulators of AP-2{gamma} Gene Transcription Biol Reprod, October 1, 2003; 69(4): 1220 - 1230. [Abstract] [Full Text] [PDF]

				Y.-H. Cheng and S. Handwerger Identification of an Enhancer of the Human Activating Protein-2{alpha} Gene That Contains a Critical Ets1 Binding Site J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3305 - 3311. [Abstract] [Full Text] [PDF]

				M. G. Carter, T. Hamatani, A. A. Sharov, C. E. Carmack, Y. Qian, K. Aiba, N. T. Ko, D. B. Dudekula, P. M. Brzoska, S. S. Hwang, et al. In Situ-Synthesized Novel Microarray Optimized for Mouse Stem Cell and Early Developmental Expression Profiling Genome Res., May 1, 2003; 13(5): 1011 - 1021. [Abstract] [Full Text] [PDF]

				M. Ben-Zimra, M. Koler, and J. Orly Transcription of Cholesterol Side-Chain Cleavage Cytochrome P450 in the Placenta: Activating Protein-2 Assumes the Role of Steroidogenic Factor-1 by Binding to an Overlapping Promoter Element Mol. Endocrinol., August 1, 2002; 16(8): 1864 - 1880. [Abstract] [Full Text] [PDF]

				U. Werling and H. Schorle Transcription Factor Gene AP-2{gamma} Essential for Early Murine Development Mol. Cell. Biol., May 1, 2002; 22(9): 3149 - 3156. [Abstract] [Full Text] [PDF]

				L. Peng and A. H. Payne AP-2gamma and the Homeodomain Protein Distal-less 3 Are Required for Placental-specific Expression of the Murine 3beta -Hydroxysteroid Dehydrogenase VI Gene, Hsd3b6 J. Biol. Chem., March 1, 2002; 277(10): 7945 - 7954. [Abstract] [Full Text] [PDF]

				H. J. Auman, T. Nottoli, O. Lakiza, Q. Winger, S. Donaldson, and T. Williams Transcription factor AP-2{gamma} is essential in the extra-embryonic lineages for early postimplantation development Development, January 6, 2002; 129(11): 2733 - 2747. [Abstract] [Full Text] [PDF]

				Y. Xia, H. Wen, H. R. Prashner, R. Chen, T. Inagami, D. F. Catanzaro, and R. E. Kellems Pregnancy-Induced Changes in Renin Gene Expression in Mice Biol Reprod, January 1, 2002; 66(1): 135 - 143. [Abstract] [Full Text]

				M. R. Dusing, A. G. Brickner, S. Y. Lowe, M. B. Cohen, and D. A. Wiginton A duodenum-specific enhancer regulates expression along three axes in the small intestine Am J Physiol Gastrointest Liver Physiol, November 1, 2000; 279(5): G1080 - G1093. [Abstract] [Full Text] [PDF]

				M. Knofler, L. Saleh, S. Bauer, R. Vasicek, G. Griesinger, H. Strohmer, H. Helmer, and P. Husslein Promoter Elements and Transcription Factors Involved in Differentiation-Dependent Human Chorionic Gonadotrophin-{alpha} Messenger Ribonucleic Acid Expression of Term Villous Trophoblasts Endocrinology, October 1, 2000; 141(10): 3737 - 3748. [Abstract] [Full Text] [PDF]

				C. LiCalsi, S. Christophe, D. J. Steger, M. Buescher, W. Fischer, and P. L. Mellon AP-2 family members regulate basal and cAMP-induced expression of human chorionic gonadotropin Nucleic Acids Res., February 15, 2000; 28(4): 1036 - 1043. [Abstract] [Full Text] [PDF]

				K. Yamada, H. Ogawa, S.-i. Honda, N. Harada, and T. Okazaki A GCM Motif Protein Is Involved in Placenta-specific Expression of Human Aromatase Gene J. Biol. Chem., November 5, 1999; 274(45): 32279 - 32286. [Abstract] [Full Text] [PDF]

				W. Johnson and J. L. Jameson AP-2 (Activating Protein 2) and Sp1 (Selective Promoter Factor 1) Regulatory Elements Play Distinct Roles in the Control of Basal Activity and Cyclic Adenosine 3',5'-Monophosphate Responsiveness of the Human Chorionic Gonadotropin-{beta} Promoter Mol. Endocrinol., November 1, 1999; 13(11): 1963 - 1975. [Abstract] [Full Text]

				A. L. Lewis, Y. Xia, S. K. Datta, J. McMillin, and R. E. Kellems Combinatorial Interactions Regulate Cardiac Expression of the Murine Adenylosuccinate Synthetase 1 Gene J. Biol. Chem., May 14, 1999; 274(20): 14188 - 14197. [Abstract] [Full Text] [PDF]