HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gunasinghe, S. K. Articles by Wright, C. F. Search for Related Content PubMed PubMed Citation Articles by Gunasinghe, S. K. Articles by Wright, C. F. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 42, 27524-27530, October 16, 1998

A Vaccinia Virus Late Transcription Factor with Biochemical and Molecular Identity to a Human Cellular Protein

Sajeevani K. Gunasinghe, Alan E. Hubbs^§, and Cynthia F. Wright

From the  Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina 29425 and the ^§ Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, D. C. 20306-6000

A factor designated VLTF-X is required to support vaccinia virus late transcription in vitro. It has been found that a late promoter DNA binding activity cochromatographs and cosediments with VLTF-X activity. Current experiments show that VLTF-X activity is present in a variety of uninfected mammalian cell types and is indistinguishable from that recovered from infected cells based upon several criteria. VLTF-X activity from both sources displays the same purification profile over phosphocellulose and DNA affinity resins and has the same sedimentation coefficient. In addition, the factors purified from both infected and uninfected cells form protein-DNA complexes of identical electrophoretic mobility in the presence of vaccinia virus late promoter-containing DNA. The affinity of these factors for the late promoter probes is identical and late promoter-specific based on competition experiments. Moreover, VLTF-X purified from both sources bound to late promoter-containing DNA in the presence or absence of MgCl₂ and ATP and formed complexes resistant to heat inactivation. These experiments offer proof that vaccinia virus factor VLTF-X is a host cell protein that supports transcription of the viral late genes.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. R. Mohamed and E. G. Niles UUUUUNU Stimulation of Vaccinia Virus Early Gene Transcription Termination: OLIGONUCLEOTIDE SEQUENCE AND STRUCTURAL REQUIREMENTS FOR STIMULATION OF PREMATURE TERMINATION IN VITRO J. Biol. Chem., October 10, 2003; 278(41): 39534 - 39541. [Abstract] [Full Text] [PDF]

				S. S. Broyles Vaccinia virus transcription J. Gen. Virol., September 1, 2003; 84(9): 2293 - 2303. [Abstract] [Full Text] [PDF]

				M. R. Mohamed and E. G. Niles UUUUUNU Oligonucleotide Stimulation of Vaccinia Virus Early Gene Transcription Termination, in Trans J. Biol. Chem., March 28, 2003; 278(14): 11794 - 11801. [Abstract] [Full Text] [PDF]

				C. A. Lackner and R. C. Condit Vaccinia Virus Gene A18R DNA Helicase Is a Transcript Release Factor J. Biol. Chem., January 14, 2000; 275(2): 1485 - 1494. [Abstract] [Full Text] [PDF]

				S. S. Broyles, X. Liu, M. Zhu, and M. Kremer Transcription Factor YY1 Is a Vaccinia Virus Late Promoter Activator J. Biol. Chem., December 10, 1999; 274(50): 35662 - 35667. [Abstract] [Full Text] [PDF]

				P. Sanz and B. Moss Identification of a transcription factor, encoded by two vaccinia virus early genes, that regulates the intermediate stage of viral gene expression PNAS, March 16, 1999; 96(6): 2692 - 2697. [Abstract] [Full Text] [PDF]

				M. R. Mohamed and E. G. Niles Interaction between Nucleoside Triphosphate Phosphohydrolase I and the H4L Subunit of the Viral RNA Polymerase Is Required for Vaccinia Virus Early Gene Transcript Release J. Biol. Chem., August 11, 2000; 275(33): 25798 - 25804. [Abstract] [Full Text] [PDF]

				M. R. Mohamed and E. G. Niles The Viral RNA Polymerase H4L Subunit Is Required for Vaccinia Virus Early Gene Transcription Termination J. Biol. Chem., June 1, 2001; 276(23): 20758 - 20765. [Abstract] [Full Text] [PDF]

				C. F. Wright, B. W. Oswald, and S. Dellis Vaccinia Virus Late Transcription Is Activated in Vitro by Cellular Heterogeneous Nuclear Ribonucleoproteins J. Biol. Chem., October 26, 2001; 276(44): 40680 - 40686. [Abstract] [Full Text] [PDF]