HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yang, L. Articles by Hickstein, D. D. Search for Related Content PubMed PubMed Citation Articles by Yang, L. Articles by Hickstein, D. D. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 43, 27761-27764, October 23, 1998

COMMUNICATION
Oncoprotein TLS Interacts with Serine-Arginine Proteins Involved in RNA Splicing

Liu Yang^§, Lisa J. Embree^§, Schickwann Tsai^¶, and Dennis D. Hickstein^§

From the  Medical Research Service, Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108, ^¶ Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, and ^§ Division of Oncology and  Molecular Medicine, University of Washington School of Medicine, Seattle, Washington 98195

The gene encoding the human TLS protein, also termed FUS, is located at the site of chromosomal translocations in human leukemias and sarcomas where it forms a chimeric fusion gene with one of several different genes. To identify interacting partners of TLS, we screened a yeast two-hybrid cDNA library constructed from mouse hematopoietic cells using the C-terminal region of TLS in the bait plasmid. Two cDNAs encoding members of the serine-arginine (SR) family of proteins were isolated. The first SR protein is the mouse homolog of human splicing factor SC35, and the second SR member is a novel 183-amino acid protein that we term TASR (TLS-associated serine-arginine protein). cDNA cloning of human TASR indicated that mouse and human TASR have identical amino acid sequences. The interactions between TLS and these two SR proteins were confirmed by co-transfection and immunoprecipitation studies. In vivo splicing assays indicated that SC35 and TASR influence splice site selection of adenovirus E1A pre-mRNA. TLS may recruit SR splicing factors to specific target genes through interaction with its C-terminal region, and chromosomal translocations that truncate the C-terminal region of TLS may prevent this interaction. Thus TLS translocations may alter RNA processing and play a role in malignant transformation.

---

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				H. Doi, K. Okamura, P. O. Bauer, Y. Furukawa, H. Shimizu, M. Kurosawa, Y. Machida, H. Miyazaki, K. Mitsui, Y. Kuroiwa, et al. RNA-binding Protein TLS Is a Major Nuclear Aggregate-interacting Protein in Huntingtin Exon 1 with Expanded Polyglutamine-expressing Cells J. Biol. Chem., March 7, 2008; 283(10): 6489 - 6500. [Abstract] [Full Text] [PDF]

				W. J. Law, K. L. Cann, and G. G. Hicks TLS, EWS and TAF15: a model for transcriptional integration of gene expression Brief Funct Genomic Proteomic, March 1, 2006; 5(1): 8 - 14. [Abstract] [Full Text] [PDF]

				M. Perani, P. Antonson, R. Hamoudi, C. J. E. Ingram, C. S. Cooper, M. D. Garrett, and G. H. Goodwin The Proto-oncoprotein SYT Interacts with SYT-interacting Protein/Co-activator Activator (SIP/CoAA), a Human Nuclear Receptor Co-activator with Similarity to EWS and TLS/FUS Family of Proteins J. Biol. Chem., December 30, 2005; 280(52): 42863 - 42876. [Abstract] [Full Text] [PDF]

				R. Fujii and T. Takumi TLS facilitates transport of mRNA encoding an actin-stabilizing protein to dendritic spines J. Cell Sci., December 15, 2005; 118(24): 5755 - 5765. [Abstract] [Full Text] [PDF]

				J. Zou, H. Ichikawa, M. L. Blackburn, H.-M. Hu, A. Zielinska-Kwiatkowska, Q. Mei, G. J. Roth, H. A. Chansky, and L. Yang The Oncogenic TLS-ERG Fusion Protein Exerts Different Effects in Hematopoietic Cells and Fibroblasts Mol. Cell. Biol., July 15, 2005; 25(14): 6235 - 6246. [Abstract] [Full Text] [PDF]

				K. Fushimi, N. Osumi, and T. Tsukahara NSSRs/TASRs/SRp38s function as splicing modulators via binding to pre-mRNAs Genes Cells, June 1, 2005; 10(6): 531 - 541. [Abstract] [Full Text] [PDF]

				K. J. Liu and R. M. Harland Inhibition of neurogenesis by SRp38, a neuroD-regulated RNA-binding protein Development, April 1, 2005; 132(7): 1511 - 1523. [Abstract] [Full Text] [PDF]

				D. Alex and K. A. W. Lee RGG-boxes of the EWS oncoprotein repress a range of transcriptional activation domains Nucleic Acids Res., March 2, 2005; 33(4): 1323 - 1331. [Abstract] [Full Text] [PDF]

				Y. Iko, T. S. Kodama, N. Kasai, T. Oyama, E. H. Morita, T. Muto, M. Okumura, R. Fujii, T. Takumi, S.-i. Tate, et al. Domain Architectures and Characterization of an RNA-binding Protein, TLS J. Biol. Chem., October 22, 2004; 279(43): 44834 - 44840. [Abstract] [Full Text] [PDF]

				B. Deneen, S. M. Welford, T. Ho, F. Hernandez, I. Kurland, and C. T. Denny PIM3 Proto-Oncogene Kinase Is a Common Transcriptional Target of Divergent EWS/ETS Oncoproteins Mol. Cell. Biol., June 1, 2003; 23(11): 3897 - 3908. [Abstract] [Full Text] [PDF]

				S. Guil, R. Gattoni, M. Carrascal, J. Abian, J. Stevenin, and M. Bach-Elias Roles of hnRNP A1, SR Proteins, and p68 Helicase in c-H-ras Alternative Splicing Regulation Mol. Cell. Biol., April 15, 2003; 23(8): 2927 - 2941. [Abstract] [Full Text] [PDF]

				K. Hartmuth, H. Urlaub, H.-P. Vornlocher, C. L. Will, M. Gentzel, M. Wilm, and R. Luhrmann Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method PNAS, December 24, 2002; 99(26): 16719 - 16724. [Abstract] [Full Text] [PDF]

				Md. T. Nasim, H. M. Chowdhury, and I. C. Eperon A double reporter assay for detecting changes in the ratio of spliced and unspliced mRNA in mammalian cells Nucleic Acids Res., October 15, 2002; 30(20): e109 - e109. [Abstract] [Full Text] [PDF]

				S. Lopato, C. Forstner, M. Kalyna, J. Hilscher, U. Langhammer, K. Indrapichate, Z. J. Lorkovic', and A. Barta Network of Interactions of a Novel Plant-specific Arg/Ser-rich Protein, atRSZ33, with atSC35-like Splicing Factors J. Biol. Chem., October 11, 2002; 277(42): 39989 - 39998. [Abstract] [Full Text] [PDF]

				A. Martini, R. La Starza, H. Janssen, C. Bilhou-Nabera, A. Corveleyn, R. Somers, A. Aventin, R. Foa, A. Hagemeijer, C. Mecucci, et al. Recurrent Rearrangement of the Ewing's Sarcoma Gene, EWSR1, or Its Homologue, TAF15, with the Transcription Factor CIZ/NMP4 in Acute Leukemia Cancer Res., October 1, 2002; 62(19): 5408 - 5412. [Abstract] [Full Text] [PDF]

				Y. Yang, C. McKerlie, S. H. Borenstein, Z. Lu, M. Schito, J. W. Chamberlain, and M. Buchwald Transgenic Expression in Mouse Lung Reveals Distinct Biological Roles for the Adenovirus Type 5 E1A 243- and 289-Amino-Acid Proteins J. Virol., July 29, 2002; 76(17): 8910 - 8919. [Abstract] [Full Text] [PDF]

				G. Dellaire, E. M. Makarov, JeffJ.M. Cowger, D. Longman, H. G. E. Sutherland, R. Luhrmann, J. Torchia, and W. A. Bickmore Mammalian PRP4 Kinase Copurifies and Interacts with Components of Both the U5 snRNP and the N-CoR Deacetylase Complexes Mol. Cell. Biol., July 15, 2002; 22(14): 5141 - 5156. [Abstract] [Full Text] [PDF]

				H. J. Baek, S. Malik, J. Qin, and R. G. Roeder Requirement of TRAP/Mediator for Both Activator-Independent and Activator-Dependent Transcription in Conjunction with TFIID-Associated TAFIIs Mol. Cell. Biol., April 15, 2002; 22(8): 2842 - 2852. [Abstract] [Full Text] [PDF]

				S. M. Welford, S. P. Hebert, B. Deneen, A. Arvand, and C. T. Denny DNA Binding Domain-independent Pathways Are Involved in EWS/FLI1-mediated Oncogenesis J. Biol. Chem., November 2, 2001; 276(45): 41977 - 41984. [Abstract] [Full Text] [PDF]

				H. Kovar, G. Jug, C. Hattinger, L. Spahn, D. N. T. Aryee, P. F. Ambros, A. Zoubek, and H. Gadner The EWS Protein Is Dispensable for Ewing Tumor Growth Cancer Res., August 1, 2001; 61(16): 5992 - 5997. [Abstract] [Full Text] [PDF]

				H. A. Chansky, M. Hu, D. D. Hickstein, and L. Yang Oncogenic TLS/ERG and EWS/Fli-1 Fusion Proteins Inhibit RNA Splicing Mediated by YB-1 Protein Cancer Res., May 1, 2001; 61(9): 3586 - 3590. [Abstract] [Full Text]

				G.-Y. Nie, Y. Li, L. Batten, B. Griffiths, J. Wang, J. K. Findlay, and L. A. Salamonsen Uterine expression of alternatively spliced mRNAs of mouse splicing factor SC35 during early pregnancy Mol. Hum. Reprod., December 1, 2000; 6(12): 1131 - 1139. [Abstract] [Full Text] [PDF]

				T. McGarvey, E. Rosonina, S. McCracken, Q. Li, R. Arnaout, E. Mientjes, J. A. Nickerson, D. Awrey, J. Greenblatt, G. Grosveld, et al. The Acute Myeloid Leukemia-associated Protein, DEK, Forms a Splicing-dependent Interaction with Exon-product Complexes J. Cell Biol., July 24, 2000; 150(2): 309 - 320. [Abstract] [Full Text] [PDF]

				Y. Hirose and J. L. Manley RNA polymerase II and the integration of nuclear events Genes & Dev., June 15, 2000; 14(12): 1415 - 1429. [Full Text]

				L. Yang, L. J. Embree, and D. D. Hickstein TLS-ERG Leukemia Fusion Protein Inhibits RNA Splicing Mediated by Serine-Arginine Proteins Mol. Cell. Biol., May 15, 2000; 20(10): 3345 - 3354. [Abstract] [Full Text]

				L. L. Knoop and S. J. Baker The Splicing Factor U1C Represses EWS/FLI-mediated Transactivation J. Biol. Chem., August 4, 2000; 275(32): 24865 - 24871. [Abstract] [Full Text] [PDF]

				L. Yang, H. A. Chansky, and D. D. Hickstein EWS{middle dot}Fli-1 Fusion Protein Interacts with Hyperphosphorylated RNA Polymerase II and Interferes with Serine-Arginine Protein-mediated RNA Splicing J. Biol. Chem., November 22, 2000; 275(48): 37612 - 37618. [Abstract] [Full Text] [PDF]

				A. Lerga, M. Hallier, L. Delva, C. Orvain, I. Gallais, J. Marie, and F. Moreau-Gachelin Identification of an RNA Binding Specificity for the Potential Splicing Factor TLS J. Biol. Chem., February 23, 2001; 276(9): 6807 - 6816. [Abstract] [Full Text] [PDF]

				L. L. Knoop and S. J. Baker EWS/FLI Alters 5'-Splice Site Selection J. Biol. Chem., June 15, 2001; 276(25): 22317 - 22322. [Abstract] [Full Text] [PDF]

				F. Kappes, K. Burger, M. Baack, F. O. Fackelmayer, and C. Gruss Subcellular Localization of the Human Proto-oncogene Protein DEK J. Biol. Chem., July 6, 2001; 276(28): 26317 - 26323. [Abstract] [Full Text] [PDF]

				L. R. Saunders, D. J. Perkins, S. Balachandran, R. Michaels, R. Ford, A. Mayeda, and G. N. Barber Characterization of Two Evolutionarily Conserved, Alternatively Spliced Nuclear Phosphoproteins, NFAR-1 and -2, That Function in mRNA Processing and Interact with the Double-stranded RNA-dependent Protein Kinase, PKR J. Biol. Chem., August 17, 2001; 276(34): 32300 - 32312. [Abstract] [Full Text] [PDF]

				J. KIM and J. PELLETIER Molecular genetics of chromosome translocations involving EWS and related family members Physiol Genomics, November 11, 1999; 1(3): 127 - 138. [Abstract] [Full Text] [PDF]