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J Biol Chem, Vol. 273, Issue 43, 28314-28321, October 23, 1998

Modification of the E-cadherin-Catenin Complex in Mitotic Madin-Darby Canine Kidney Epithelial Cells

From the Department of Molecular Embryology, Max-Planck Institute of Immunobiology, Stübeweg 51, D-79108 Freiburg, Germany

One of the hallmarks of polarized epithelial cells undergoing mitosis is their rounded morphology. This phenotype correlates with a reduced cell-substratum adhesion, apparently caused by a modulation of integrin function. However, it is still unclear whether the cadherin-mediated cell-cell adhesion is affected as well. To address this question, the cadherin complex was analyzed in different cell cycle stages of Madin-Darby canine kidney cells. By immunofluorescence, mitotic Madin-Darby canine kidney cells showed an increased staining of E-cadherin and the catenins (-catenin, -catenin, plakoglobin, p120^ctn) in the cytosol, suggesting a reorganization of the cadherin-catenin complex during mitosis. Biochemical analysis revealed that the overall amount of these components, as well as the proportion of the complex associated with the actin cytoskeleton, remained unchanged in mitotic cells. However, we found evidence for an internalization of E-cadherin during mitosis. In addition, the cadherin-catenin complex was analyzed for mitosis-specific changes in phosphorylation. We report a decrease in the tyrosine phosphorylation of -catenin, plakoglobin, and p120^ctn during mitosis. Moreover, we observed a mitosis-specific Ser/Thr-phosphorylation of p120^ctn, as detected by the MPM-2 antibody. Hence, the cadherin/catenin complex is a target for different posttranslational modifications during mitosis, which may also have a profound impact on cadherin-mediated cell-cell adhesion.

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