|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 43, 28444-28453, October 23, 1998
From the The neural chondroitin sulfate (CS) proteoglycan
(PG) DSD-1-PG was originally identified with the monoclonal antibody
(mAb) 473HD. It promotes neurite outgrowth of hippocampal neurons when coated as a substrate in the presence of polycations. This effect is
inhibited by mAb 473HD that specifically recognizes the DSD-1 epitope.
The DSD-1 epitope is also detectable in CS-C and CS-D preparations from
shark cartilage but not in other chondroitin sulfates that are
structurally related and differ in their sulfation patterns.
Non-sulfated DSD-1-PG and chemically desulfated CS-D were not
recognized by mAb 473HD, suggesting that the DSD-1 epitope depends on
sulfation. It was possible to enrich DSD-1 epitope-bearing carbohydrates and D disaccharide units from CS-C and CS-D preparations on a mAb 473HD affinity matrix. This indicates that the DSD-1 epitope
represents a distinct glycosaminoglycan structure containing D units.
The analysis of glycosaminoglycan digestion products by high pressure
liquid chromatography revealed that DSD-1-PG preparations contain a
unique D disaccharide unit as well as an A, a C, and a non-sulfated
disaccharide unit. In neurite outgrowth assays with hippocampal
neurons, substrate-bound CS-D promoted neurite outgrowth, whereas CS-A,
CS-B, or CS-C did not. This effect of CS-D was inhibited by mAb 473HD.
DSD-1 epitope-enriched fractions obtained from CS-D and CS-C promoted
neurite outgrowth, whereas CS-C had no such effect prior to enrichment
on the mAb 473HD matrix. Based on these findings we conclude that the
DSD-1 epitope by itself is sufficient to promote neurite outgrowth and
that this activity is possibly associated with D motifs.
The DSD-1 Carbohydrate Epitope Depends on Sulfation, Correlates
with Chondroitin Sulfate D Motifs, and Is Sufficient to Promote
Neurite Outgrowth
,,¶
Department of Neurobiology, University of
Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany, the
§ Department of Biochemistry, Kobe Pharmaceutical
University, Higashinada-ku 20, Kobe 658-8558, Japan, and
¶ Laboratoire de Neurobiologie du Développment et
de la Régéneration, UPR 1352, Centre de Neurochimie du CNRS
et Université Louis Pasteur, F-67084 Strasbourg, France
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Akita, A. von Holst, Y. Furukawa, T. Mikami, K. Sugahara, and A. Faissner Expression of Multiple Chondroitin/Dermatan Sulfotransferases in the Neurogenic Regions of the Embryonic and Adult Central Nervous System Implies That Complex Chondroitin Sulfates Have a Role in Neural Stem Cell Maintenance Stem Cells, March 1, 2008; 26(3): 798 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Fongmoon, A. K. Shetty, Basappa, S. Yamada, M. Sugiura, P. Kongtawelert, and K. Sugahara Chondroitinase-mediated Degradation of Rare 3-O-Sulfated Glucuronic Acid in Functional Oversulfated Chondroitin Sulfate K and E J. Biol. Chem., December 21, 2007; 282(51): 36895 - 36904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamaguchi, S. Ohtake, K. Kimata, and O. Habuchi Molecular Cloning of Squid N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase and Synthesis of a Unique Chondroitin Sulfate Containing E D Hybrid Tetrasaccharide Structure by the Recombinant Enzyme Glycobiology, December 1, 2007; 17(12): 1365 - 1376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pothacharoen, K. Kalayanamitra, S. S. Deepa, S. Fukui, T. Hattori, N. Fukushima, T. Hardingham, P. Kongtawelert, and K. Sugahara Two Related but Distinct Chondroitin Sulfate Mimetope Octasaccharide Sequences Recognized by Monoclonal Antibody WF6 J. Biol. Chem., November 30, 2007; 282(48): 35232 - 35246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamada, H. Morimoto, T. Fujisawa, and K. Sugahara Glycosaminoglycans in Hydra magnipapillata (Hydrozoa, Cnidaria): demonstration of chondroitin in the developing nematocyst, the sting organelle, and structural characterization of glycosaminoglycans Glycobiology, August 1, 2007; 17(8): 886 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Purushothaman, J. Fukuda, S. Mizumoto, G. B. ten Dam, T. H. van Kuppevelt, H. Kitagawa, T. Mikami, and K. Sugahara Functions of Chondroitin Sulfate/Dermatan Sulfate Chains in Brain Development: CRITICAL ROLES OF E AND iE DISACCHARIDE UNITS RECOGNIZED BY A SINGLE CHAIN ANTIBODY GD3G7 J. Biol. Chem., July 6, 2007; 282(27): 19442 - 19452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S Deepa, S. Yamada, S. Fukui, and K. Sugahara Structural determination of novel sulfated octasaccharides isolated from chondroitin sulfate of shark cartilage and their application for characterizing monoclonal antibody epitopes Glycobiology, June 1, 2007; 17(6): 631 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Li, A. K. Shetty, and K. Sugahara Neuritogenic Activity of Chondroitin/Dermatan Sulfate Hybrid Chains of Embryonic Pig Brain and Their Mimicry from Shark Liver: INVOLVEMENT OF THE PLEIOTROPHIN AND HEPATOCYTE GROWTH FACTOR SIGNALING PATHWAYS J. Biol. Chem., February 2, 2007; 282(5): 2956 - 2966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mitsunaga, T. Mikami, S. Mizumoto, J. Fukuda, and K. Sugahara Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains in the Development of Cerebellum: SPATIOTEMPORAL REGULATION OF THE EXPRESSION OF CRITICAL DISULFATED DISACCHARIDES BY SPECIFIC SULFOTRANSFERASES J. Biol. Chem., July 14, 2006; 281(28): 18942 - 18952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Deepa, D. Carulli, C. Galtrey, K. Rhodes, J. Fukuda, T. Mikami, K. Sugahara, and J. W. Fawcett Composition of Perineuronal Net Extracellular Matrix in Rat Brain: A DIFFERENT DISACCHARIDE COMPOSITION FOR THE NET-ASSOCIATED PROTEOGLYCANS J. Biol. Chem., June 30, 2006; 281(26): 17789 - 17800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. C. Miller, C. E. Costello, A. Malmstrom, and J. Zaia A tandem mass spectrometric approach to determination of chondroitin/dermatan sulfate oligosaccharide glycoforms Glycobiology, June 1, 2006; 16(6): 502 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maccarana, B. Olander, J. Malmstrom, K. Tiedemann, R. Aebersold, U. Lindahl, J.-p. Li, and A. Malmstrom Biosynthesis of Dermatan Sulfate: CHONDROITIN-GLUCURONATE C5-EPIMERASE IS IDENTICAL TO SART2 J. Biol. Chem., April 28, 2006; 281(17): 11560 - 11568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. von Holst, S. Sirko, and A. Faissner The Unique 473HD-Chondroitinsulfate Epitope Is Expressed by Radial Glia and Involved in Neural Precursor Cell Proliferation J. Neurosci., April 12, 2006; 26(15): 4082 - 4094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Liu, C.-H. Chau, H. Liu, B. R. Jang, X. Li, Y.-S. Chan, and D. K. Y. Shum Upregulation of chondroitin 6-sulphotransferase-1 facilitates Schwann cell migration during axonal growth. J. Cell Sci., March 1, 2006; 119(Pt 5): 933 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ohtake, K. Kimata, and O. Habuchi Recognition of Sulfation Pattern of Chondroitin Sulfate by Uronosyl 2-O-Sulfotransferase J. Biol. Chem., November 25, 2005; 280(47): 39115 - 39123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Bao, T. Muramatsu, and K. Sugahara Demonstration of the Pleiotrophin-binding Oligosaccharide Sequences Isolated from Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains of Embryonic Pig Brains J. Biol. Chem., October 21, 2005; 280(42): 35318 - 35328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Bao, M. S. G. Pavao, J. C. dos Santos, and K. Sugahara A Functional Dermatan Sulfate Epitope Containing Iduronate(2-O-sulfate){alpha}1-3GalNAc(6-O-sulfate) Disaccharide in the Mouse Brain: DEMONSTRATION USING A NOVEL MONOCLONAL ANTIBODY RAISED AGAINST DERMATAN SULFATE OF ASCIDIAN ASCIDIA NIGRA J. Biol. Chem., June 17, 2005; 280(24): 23184 - 23193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ito, M. Hikino, Y. Yajima, T. Mikami, S. Sirko, A. von Holst, A. Faissner, S. Fukui, and K. Sugahara Structural characterization of the epitopes of the monoclonal antibodies 473HD, CS-56, and MO-225 specific for chondroitin sulfate D-type using the oligosaccharide library Glycobiology, June 1, 2005; 15(6): 593 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Bao, T. Mikami, S. Yamada, A. Faissner, T. Muramatsu, and K. Sugahara Heparin-binding Growth Factor, Pleiotrophin, Mediates Neuritogenic Activity of Embryonic Pig Brain-derived Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains J. Biol. Chem., March 11, 2005; 280(10): 9180 - 9191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Nandini, N. Itoh, and K. Sugahara Novel 70-kDa Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains with a Unique Heterogenous Sulfation Pattern from Shark Skin, Which Exhibit Neuritogenic Activity and Binding Activities for Growth Factors and Neurotrophic Factors J. Biol. Chem., February 11, 2005; 280(6): 4058 - 4069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Nandini, T. Mikami, M. Ohta, N. Itoh, F. Akiyama-Nambu, and K. Sugahara Structural and Functional Characterization of Oversulfated Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains from the Notochord of Hagfish: NEURITOGENIC AND BINDING ACTIVITIES FOR GROWTH FACTORS AND NEUROTROPHIC FACTORS J. Biol. Chem., December 3, 2004; 279(49): 50799 - 50809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Bao, S. Nishimura, T. Mikami, S. Yamada, N. Itoh, and K. Sugahara Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains from Embryonic Pig Brain, Which Contain a Higher Proportion of L-Iduronic Acid than Those from Adult Pig Brain, Exhibit Neuritogenic and Growth Factor Binding Activities J. Biol. Chem., March 12, 2004; 279(11): 9765 - 9776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hikino, T. Mikami, A. Faissner, A.-C. E. S. Vilela-Silva, M. S. G. Pavao, and K. Sugahara Oversulfated Dermatan Sulfate Exhibits Neurite Outgrowth-promoting Activity toward Embryonic Mouse Hippocampal Neurons: IMPLICATIONS OF DERMATAN SULFATE IN NEURITOGENESIS IN THE BRAIN J. Biol. Chem., October 31, 2003; 278(44): 43744 - 43754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Maeda, J. He, Y. Yajima, T. Mikami, K. Sugahara, and T. Yabe Heterogeneity of the Chondroitin Sulfate Portion of Phosphacan/6B4 Proteoglycan Regulates Its Binding Affinity for Pleiotrophin/Heparin Binding Growth-associated Molecule J. Biol. Chem., September 12, 2003; 278(37): 35805 - 35811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Garwood, N. Heck, F. Reichardt, and A. Faissner Phosphacan Short Isoform, a Novel Non-proteoglycan Variant of Phosphacan/Receptor Protein Tyrosine Phosphatase-{beta}, Interacts with Neuronal Receptors and Promotes Neurite Outgrowth J. Biol. Chem., June 20, 2003; 278(26): 24164 - 24173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Deepa, Y. Umehara, S. Higashiyama, N. Itoh, and K. Sugahara Specific Molecular Interactions of Oversulfated Chondroitin Sulfate E with Various Heparin-binding Growth Factors. IMPLICATIONS AS A PHYSIOLOGICAL BINDING PARTNER IN THE BRAIN AND OTHER TISSUES J. Biol. Chem., November 8, 2002; 277(46): 43707 - 43716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Zanetta, R. Bindeus, G. Normand, V. Durier, P. Lagant, E. Maes, and G. Vergoten Evidence for a Lectin Activity for Human Interleukin 3 and Modeling of Its Carbohydrate Recognition Domain J. Biol. Chem., October 4, 2002; 277(41): 38764 - 38771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Becker and T. Becker Repellent Guidance of Regenerating Optic Axons by Chondroitin Sulfate Glycosaminoglycans in Zebrafish J. Neurosci., February 1, 2002; 22(3): 842 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Harroch, M. Palmeri, J. Rosenbluth, A. Custer, M. Okigaki, P. Shrager, M. Blum, J. D. Buxbaum, and J. Schlessinger No Obvious Abnormality in Mice Deficient in Receptor Protein Tyrosine Phosphatase beta Mol. Cell. Biol., October 15, 2000; 20(20): 7706 - 7715. [Abstract] [Full Text]
|
|
|
|
|
|
J. Garwood, O. Schnadelbach, A. Clement, K. Schutte, A. Bach, and A. Faissner DSD-1-Proteoglycan Is the Mouse Homolog of Phosphacan and Displays Opposing Effects on Neurite Outgrowth Dependent on Neuronal Lineage J. Neurosci., May 15, 1999; 19(10): 3888 - 3899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ueoka, N. Kaneda, I. Okazaki, S. Nadanaka, T. Muramatsu, and K. Sugahara Neuronal Cell Adhesion, Mediated by the Heparin-binding Neuroregulatory Factor Midkine, Is Specifically Inhibited by Chondroitin Sulfate E. STRUCTURAL AND FUNCTIONAL IMPLICATIONS OF THE OVER-SULFATED CHONDROITIN SULFATE J. Biol. Chem., November 22, 2000; 275(48): 37407 - 37413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Uchimura, K. Kadomatsu, H. Nishimura, H. Muramatsu, E. Nakamura, N. Kurosawa, O. Habuchi, F. M. El-Fasakhany, Y. Yoshikai, and T. Muramatsu Functional Analysis of the Chondroitin 6-Sulfotransferase Gene in Relation to Lymphocyte Subpopulations, Brain Development, and Oversulfated Chondroitin Sulfates J. Biol. Chem., January 4, 2002; 277(2): 1443 - 1450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tsuchida, J. Shioi, S. Yamada, G. Boghosian, A. Wu, H. Cai, K. Sugahara, and N. K. Robakis Appican, the Proteoglycan Form of the Amyloid Precursor Protein, Contains Chondroitin Sulfate E in the Repeating Disaccharide Region and 4-O-Sulfated Galactose in the Linkage Region J. Biol. Chem., September 28, 2001; 276(40): 37155 - 37160. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |