HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lehmann, M. Articles by Korge, G. Search for Related Content PubMed PubMed Citation Articles by Lehmann, M. Articles by Korge, G. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 43, 28504-28509, October 23, 1998

The Pipsqueak Protein of Drosophila melanogaster Binds to GAGA Sequences through a Novel DNA-binding Domain

From the Institute of Genetics, Free University Berlin, Arnimallee 7, D-14195 Berlin, Germany

Pipsqueak (Psq) belongs to a family of proteins defined by a phylogenetically old protein-protein interaction motif. Like the GAGA factor and other members of this family, Psq is an important developmental regulator in Drosophila, having pleiotropic functions during oogenesis, embryonic pattern formation, and adult development. The GAGA factor controls the transcriptional activation of homeotic genes and other genes by binding to control elements containing the GAGAG consensus motif. Binding is associated with formation of an open chromatin structure that makes the control regions accessible to transcriptional activators. We show here that Psq contains a novel DNA-binding domain, which binds, like the GAGA factor zinc finger DNA-binding domain, to target sites containing the GAGAG consensus motif. Binding is suppressed, as in the GAGA factor and other proteins of the family, by the associated protein-protein interaction motif. The DNA-binding domain, which we call the Psq domain, is identical with a previously identified region consisting of four tandem repeats of a conserved 50-amino acid sequence, the Psq motif. The Psq domain seems to be structurally related to known DNA-binding domains, both in its repetitive character and in the putative three--helix structure of the Psq motif, but it lacks the conserved sequence signatures of the classical eukaryotic DNA-binding motifs. Psq may thus represent the prototype of a new family of DNA-binding proteins.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				D. Sambandan, A. Yamamoto, J.-J. Fanara, T. F. C. Mackay, and R. R. H. Anholt Dynamic Genetic Interactions Determine Odor-Guided Behavior in Drosophila melanogaster Genetics, November 1, 2006; 174(3): 1349 - 1363. [Abstract] [Full Text] [PDF]

				A. Blastyak, R. K. Mishra, F. Karch, and H. Gyurkovics Efficient and Specific Targeting of Polycomb Group Proteins Requires Cooperative Interaction between Grainyhead and Pleiohomeotic Mol. Cell. Biol., February 15, 2006; 26(4): 1434 - 1444. [Abstract] [Full Text] [PDF]

				J. L. Brown, D. J. Grau, S. K. DeVido, and J. A. Kassis An Sp1/KLF binding site is important for the activity of a Polycomb group response element from the Drosophila engrailed gene Nucleic Acids Res., September 9, 2005; 33(16): 5181 - 5189. [Abstract] [Full Text] [PDF]

				S. Pauli, H. M. Rothnie, G. Chen, X. He, and T. Hohn The Cauliflower Mosaic Virus 35S Promoter Extends into the Transcribed Region J. Virol., November 15, 2004; 78(22): 12120 - 12128. [Abstract] [Full Text] [PDF]

				C. Lours, O. Bardot, D. Godt, F. A. Laski, and J.-L. Couderc The Drosophila melanogaster BTB proteins bric a brac bind DNA through a composite DNA binding domain containing a pipsqueak and an AT-Hook motif Nucleic Acids Res., September 15, 2003; 31(18): 5389 - 5398. [Abstract] [Full Text] [PDF]

				J.-L. Couderc, D. Godt, S. Zollman, J. Chen, M. Li, S. Tiong, S. E. Cramton, I. Sahut-Barnola, and F. A. Laski The bric a brac locus consists of two paralogous genes encoding BTB/POZ domain proteins and acts as a homeotic and morphogenetic regulator of imaginal development in Drosophila Development, March 7, 2003; 129(10): 2419 - 2433. [Abstract] [Full Text] [PDF]

				M. Faucheux, J.-Y. Roignant, S. Netter, J. Charollais, C. Antoniewski, and L. Theodore batman Interacts with Polycomb and trithorax Group Genes and Encodes a BTB/POZ Protein That Is Included in a Complex Containing GAGA Factor Mol. Cell. Biol., February 15, 2003; 23(4): 1181 - 1195. [Abstract] [Full Text] [PDF]

				A. Schwendemann and M. Lehmann Pipsqueak and GAGA factor act in concert as partners at homeotic and many other loci PNAS, October 1, 2002; 99(20): 12883 - 12888. [Abstract] [Full Text] [PDF]

				D.-H. Huang, Y.-L. Chang, C.-C. Yang, I-C. Pan, and B. King pipsqueak Encodes a Factor Essential for Sequence-Specific Targeting of a Polycomb Group Protein Complex Mol. Cell. Biol., September 1, 2002; 22(17): 6261 - 6271. [Abstract] [Full Text] [PDF]

				J. Americo, M. Whiteley, J. L. Brown, M. Fujioka, J. B. Jaynes, and J. A. Kassis A Complex Array of DNA-Binding Proteins Required for Pairing-Sensitive Silencing by a Polycomb Group Response Element From the Drosophila engrailed Gene Genetics, April 1, 2002; 160(4): 1561 - 1571. [Abstract] [Full Text] [PDF]

				B. P. Berman, Y. Nibu, B. D. Pfeiffer, P. Tomancak, S. E. Celniker, M. Levine, G. M. Rubin, and M. B. Eisen Exploiting transcription factor binding site clustering to identify cis-regulatory modules involved in pattern formation in the Drosophila genome PNAS, January 22, 2002; 99(2): 757 - 762. [Abstract] [Full Text] [PDF]

				K. Shim, K. J. Blake, J. Jack, and M. A. Krasnow The Drosophila ribbon gene encodes a nuclear BTB domain protein that promotes epithelial migration and morphogenesis Development, December 1, 2001; 128(23): 4923 - 4933. [Abstract] [Full Text] [PDF]

				A. M. Volpe, H. Horowitz, C. M. Grafer, S. M. Jackson, and C. A. Berg Drosophila rhino Encodes a Female-Specific Chromo-domain Protein That Affects Chromosome Structure and Egg Polarity Genetics, November 1, 2001; 159(3): 1117 - 1134. [Abstract] [Full Text] [PDF]

				N. Renault, K. King-Jones, and M. Lehmann Downregulation of the tissue-specific transcription factor Fork head by Broad-Complex mediates a stage-specific hormone response Development, October 1, 2001; 128(19): 3729 - 3737. [Abstract] [Full Text] [PDF]

				J. W. Hodgson, B. Argiropoulos, and H. W. Brock Site-Specific Recognition of a 70-Base-Pair Element Containing d(GA)n Repeats Mediates bithoraxoid Polycomb Group Response Element-Dependent Silencing Mol. Cell. Biol., July 15, 2001; 21(14): 4528 - 4543. [Abstract] [Full Text] [PDF]

				M. L. Espinas, E. Jimenez-Garcia, A. Vaquero, S. Canudas, J. Bernues, and F. Azorin The N-terminal POZ Domain of GAGA Mediates the Formation of Oligomers That Bind DNA with High Affinity and Specificity J. Biol. Chem., June 4, 1999; 274(23): 16461 - 16469. [Abstract] [Full Text] [PDF]

				A. Vaquero, M. L. Espinas, F. Azorin, and J. Bernues Functional Mapping of the GAGA Factor Assigns Its Transcriptional Activity to the C-terminal Glutamine-rich Domain J. Biol. Chem., June 23, 2000; 275(26): 19461 - 19468. [Abstract] [Full Text] [PDF]