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J Biol Chem, Vol. 273, Issue 44, 28553-28556, October 30, 1998

COMMUNICATION
Cloning of cDNA Encoding a Novel Mouse DNA Topoisomerase III (Topo III) Possessing Negatively Supercoiled DNA Relaxing Activity, Whose Message Is Highly Expressed in the Testis

Takahiko Seki^§, Masayuki Seki, Ryoko Onodera, Toshiaki Katada^§, and Takemi Enomoto

From the  Department of Molecular Cell Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578 and the ^§ Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-8654, Japan

We cloned cDNA encoding a novel mouse homologue of DNA topoisomerase III (mTOP3). The nucleotide sequence contains an open reading frame of 863 amino acids, and the deduced molecular mass of the coded protein is 96.9 kDa. The overall sequence of mTOP3 has a 48 and 36% identity with mouse TOP3 at the nucleotide and amino acid level, respectively. DNA topoisomerase III was expressed using a baculovirus expression system and purified. The purified DNA topoisomerase III had activity to relax negatively supercoiled DNA. Relaxation of supercoiled DNA was partial at 37 °C and complete relaxation was observed at higher temperatures. mTOP3 mRNA was strongly expressed in the testis and relatively strongly in the brain. The levels of TOP3 mRNA in the testis increased slightly 14 days and considerably 17 days after birth, when the cells in the pachytene phase begin to appear and increase.

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Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.

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