| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 44, 28721-28732, October 30, 1998
3
2 but Not
34 Acetylcholine Receptors Stably Transfected in Human Embryonic
Kidney Cells
,,,,,
From the Human nicotinic acetylcholine receptor (AChR)
subtypes Department of Neuroscience, University of
Pennsylvania Medical School, Philadelphia, Pennsylvania 19104-6074 and ¶ Department of Physiology and Optics, University of Alabama,
Birmingham, Alabama 35294-4390
3
2, 325, 34, and 345 were stably
expressed in cells derived from the human embryonic kidney cell line
293. 34 AChRs were found in prominent 2-µm patches on the cell
surface, whereas most 32 AChRs were more diffusely distributed.
The functional properties of the 3 AChRs in tsA201 cells were
characterized by whole cell patch clamp using both acetylcholine and
nicotine as agonists. Nicotine was a partial agonist on 34 AChRs
and nearly a full agonist on 325 AChRs. Chronic exposure of
cells expressing 32 AChRs or 325 AChRs to nicotine or
carbamylcholine increased their amount up to 24-fold but had no effect
on the amount of 34 or 345 AChRs, i.e. the
up-regulation of 3 AChRs depended on the presence of 2 but not
4 subunits in the AChRs. This was also found to be true of 3
AChRs in the human neuroblastoma SH-SY5Y. In the absence of nicotine,
32 AChRs were expressed at much lower levels than 34 AChRs,
but in the presence of nicotine, the amount of 32 AChRs exceeded
that of 34 AChRs. Up-regulation was seen for both total AChRs and
surface AChRs. Up-regulated 32 AChRs were functional. The
nicotinic antagonists curare and dihydro--erythroidine also
up-regulated 32 AChRs, but only by 3-5-fold. The channel blocker
mecamylamine did not cause up-regulation of 32 AChRs and
inhibited up-regulation by nicotine. Our data suggest that
up-regulation of 32 AChRs in these lines by nicotine results from
both increased subunit assembly and decreased AChR turnover.
This article has been cited by other articles:
|
|
 |
|
  J. Arredondo, A. I. Chernyavsky, D. L. Jolkovsky, K. E. Pinkerton, and S. A. Grando Receptor-mediated tobacco toxicity: acceleration of sequential expression of {alpha}5 and {alpha}7 nicotinic receptor subunits in oral keratinocytes exposed to cigarette smoke FASEB J, May 1, 2008; 22(5): 1356 - 1368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Walsh, A. P. Govind, R. Mastro, J. C. Hoda, D. Bertrand, Y. Vallejo, and W. N. Green Up-regulation of Nicotinic Receptors by Nicotine Varies with Receptor Subtype J. Biol. Chem., March 7, 2008; 283(10): 6022 - 6032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Rezvani, Y. Teng, D. Shim, and M. De Biasi Nicotine Regulates Multiple Synaptic Proteins by Inhibiting Proteasomal Activity J. Neurosci., September 26, 2007; 27(39): 10508 - 10519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Wada, M. Naito, H. Kenmochi, H. Tsuneki, and T. Sasaoka Chronic Nicotine Exposure Enhances Insulin-Induced Mitogenic Signaling via Up-Regulation of {alpha}7 Nicotinic Receptors in Isolated Rat Aortic Smooth Muscle Cells Endocrinology, February 1, 2007; 148(2): 790 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Tumkosit, A. Kuryatov, J. Luo, and J. Lindstrom beta3 Subunits Promote Expression and Nicotine-Induced Up-Regulation of Human Nicotinic {alpha}6* Nicotinic Acetylcholine Receptors Expressed in Transfected Cell Lines Mol. Pharmacol., October 1, 2006; 70(4): 1358 - 1368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Moroni, R. Zwart, E. Sher, B. K. Cassels, and I. Bermudez {alpha}4beta2 Nicotinic Receptors with High and Low Acetylcholine Sensitivity: Pharmacology, Stoichiometry, and Sensitivity to Long-Term Exposure to Nicotine Mol. Pharmacol., August 1, 2006; 70(2): 755 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ohtani, T. Oka, M. Badyuk, Y. Xiao, K. J. Kellar, and J. W. Daly Mouse beta-TC6 Insulinoma Cells: High Expression of Functional {alpha}3beta4 Nicotinic Receptors Mediating Membrane Potential, Intracellular Calcium, and Insulin Release Mol. Pharmacol., March 1, 2006; 69(3): 899 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kuryatov, J. Luo, J. Cooper, and J. Lindstrom Nicotine Acts as a Pharmacological Chaperone to Up-Regulate Human {alpha}4{beta}2 Acetylcholine Receptors Mol. Pharmacol., December 1, 2005; 68(6): 1839 - 1851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Jo, D. Wiedl, and L. W. Role Cholinergic Modulation of Appetite-Related Synapses in Mouse Lateral Hypothalamic Slice J. Neurosci., November 30, 2005; 25(48): 11133 - 11144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Ficklin, S. Zhao, and G. Feng Ubiquilin-1 Regulates Nicotine-induced Up-regulation of Neuronal Nicotinic Acetylcholine Receptors J. Biol. Chem., October 7, 2005; 280(40): 34088 - 34095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Obaid, M. E. Nelson, J. Lindstrom, and B. M. Salzberg Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig enteric nervous system J. Exp. Biol., August 1, 2005; 208(15): 2981 - 3001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Free, S. B. McKay, P. D. Gottlieb, R. T. Boyd, and D. B. McKay Expression of Native {alpha}3{beta}4* Neuronal Nicotinic Receptors: Binding and Functional Studies Investigating Turnover of Surface and Intracellular Receptor Populations Mol. Pharmacol., June 1, 2005; 67(6): 2040 - 2048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Alkondon and E. X. Albuquerque Nicotinic Receptor Subtypes in Rat Hippocampal Slices Are Differentially Sensitive to Desensitization and Early in Vivo Functional Up-Regulation by Nicotine and to Block by Bupropion J. Pharmacol. Exp. Ther., May 1, 2005; 313(2): 740 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Abdrakhmanova, L. Cleemann, J. Lindstrom, and M. Morad Differential Modulation of {beta}2 and {beta}4 Subunits of Human Neuronal Nicotinic Acetylcholine Receptors by Acidification Mol. Pharmacol., August 1, 2004; 66(2): 347 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xiao and K. J. Kellar The Comparative Pharmacology and Up-Regulation of Rat Neuronal Nicotinic Receptor Subtype Binding Sites Stably Expressed in Transfected Mammalian Cells J. Pharmacol. Exp. Ther., July 1, 2004; 310(1): 98 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sallette, S. Bohler, P. Benoit, M. Soudant, S. Pons, N. Le Novere, J.-P. Changeux, and P. J. Corringer An Extracellular Protein Microdomain Controls Up-regulation of Neuronal Nicotinic Acetylcholine Receptors by Nicotine J. Biol. Chem., April 30, 2004; 279(18): 18767 - 18775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wang, J. T. Hackett, M. E. Cox, M. van Hoek, J. M. Lindstrom, and S. J. Parsons Regulation of the Neuronal Nicotinic Acetylcholine Receptor by Src Family Tyrosine Kinases J. Biol. Chem., March 5, 2004; 279(10): 8779 - 8786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Parker, Y. Fu, K. McAllen, J. Luo, J. M. McIntosh, J. M. Lindstrom, and B. M. Sharp Up-Regulation of Brain Nicotinic Acetylcholine Receptors in the Rat during Long-Term Self-Administration of Nicotine: Disproportionate Increase of the {alpha}6 Subunit Mol. Pharmacol., March 1, 2004; 65(3): 611 - 622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Nashmi, M. E. Dickinson, S. McKinney, M. Jareb, C. Labarca, S. E. Fraser, and H. A. Lester Assembly of {alpha}4{beta}2 Nicotinic Acetylcholine Receptors Assessed with Functional Fluorescently Labeled Subunits: Effects of Localization, Trafficking, and Nicotine-Induced Upregulation in Clonal Mammalian Cells and in Cultured Midbrain Neurons J. Neurosci., December 17, 2003; 23(37): 11554 - 11567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. N. Nguyen, B. A. Rasmussen, and D. C. Perry Subtype-Selective Up-Regulation by Chronic Nicotine of High-Affinity Nicotinic Receptors in Rat Brain Demonstrated by Receptor Autoradiography J. Pharmacol. Exp. Ther., December 1, 2003; 307(3): 1090 - 1097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Iho, Y. Tanaka, R. Takauji, C. Kobayashi, I. Muramatsu, H. Iwasaki, K. Nakamura, Y. Sasaki, K. Nakao, and T. Takahashi Nicotine induces human neutrophils to produce IL-8 through the generation of peroxynitrite and subsequent activation of NF-{kappa}B J. Leukoc. Biol., November 1, 2003; 74(5): 942 - 951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Avila, M. I. Davila-Garcia, V. S. Ascarrunz, Y. Xiao, and K. J. Kellar Differential Regulation of Nicotinic Acetylcholine Receptors in PC12 Cells by Nicotine and Nerve Growth Factor Mol. Pharmacol., October 1, 2003; 64(4): 974 - 986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. LINDSTROM Nicotinic Acetylcholine Receptors of Muscles and Nerves: Comparison of Their Structures, Functional Roles, and Vulnerability to Pathology Ann. N.Y. Acad. Sci., September 1, 2003; 998(1): 41 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cohen, O. E. Bergis, F. Galli, A. W. Lochead, S. Jegham, B. Biton, J. Leonardon, P. Avenet, F. Sgard, F. Besnard, et al. SSR591813, a Novel Selective and Partial {alpha}4{beta}2 Nicotinic Receptor Agonist with Potential as an Aid to Smoking Cessation J. Pharmacol. Exp. Ther., July 1, 2003; 306(1): 407 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Nelson, A. Kuryatov, C. H. Choi, Y. Zhou, and J. Lindstrom Alternate Stoichiometries of alpha 4beta 2 Nicotinic Acetylcholine Receptors Mol. Pharmacol., February 1, 2003; 63(2): 332 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Harkness and N. S. Millar Changes in Conformation and Subcellular Distribution of alpha 4beta 2 Nicotinic Acetylcholine Receptors Revealed by Chronic Nicotine Treatment and Expression of Subunit Chimeras J. Neurosci., December 1, 2002; 22(23): 10172 - 10181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Plenge, E. T. Mellerup, and G. Wortwein Characterization of Epibatidine Binding to Medial Habenula: Potential Role in Analgesia J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 759 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Rush, A. Kuryatov, M. E. Nelson, and J. Lindstrom First and Second Transmembrane Segments of alpha 3, alpha 4, beta 2, and beta 4 Nicotinic Acetylcholine Receptor Subunits Influence the Efficacy and Potency of Nicotine Mol. Pharmacol., June 1, 2002; 61(6): 1416 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, E. F. R. Pereira, A. D. J. Maus, N. S. Ostlie, D. Navaneetham, S. Lei, E. X. Albuquerque, and B. M. Conti-Fine Human Bronchial Epithelial and Endothelial Cells Express alpha 7 Nicotinic Acetylcholine Receptors Mol. Pharmacol., December 1, 2001; 60(6): 1201 - 1209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Meyer, Y. Xiao, and K. J. Kellar Agonist Regulation of Rat alpha 3beta 4 Nicotinic Acetylcholine Receptors Stably Expressed in Human Embryonic Kidney 293 Cells Mol. Pharmacol., September 1, 2001; 60(3): 568 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Girod and L. W. Role Long-Lasting Enhancement of Glutamatergic Synaptic Transmission by Acetylcholine Contrasts with Response Adaptation after Exposure to Low-Level Nicotine J. Neurosci., July 15, 2001; 21(14): 5182 - 5190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Obaid, T. Koyano, J. Lindstrom, T. Sakai, and B. M. Salzberg Spatiotemporal Patterns of Activity in an Intact Mammalian Network with Single-Cell Resolution: Optical Studies of Nicotinic Activity in an Enteric Plexus J. Neurosci., April 15, 1999; 19(8): 3073 - 3093. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
