| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 44, 29224-29229, October 30, 1998
From the Departments of The B cell-specific, sequence-specific duplex
DNA-binding protein LR1 is a transcriptional activator and may also
function in heavy chain class switch recombination. LR1 is composed of two polypeptides, a 106-kDa subunit that is nucleolin, and a 45-kDa subunit that we now show to be a specific isoform of hnRNP D. hnRNP D
and nucleolin both contain canonical RNA binding domains (RBDs also
called RRMs) and Arg-Gly-Gly (RGG) motifs. Although these motifs are
not commonly associated with sequence-specific recognition of duplex
DNA, nonetheless LR1 binds duplex DNA with high affinity
(KD = 1.8 nM) and clear sequence
specificity. Two RBD-RGG proteins can therefore combine to produce a
sequence-specific duplex DNA-binding protein.
A Specific Isoform of hnRNP D Interacts with DNA in the LR1
Heterodimer: Canonical RNA Binding Motifs in a Sequence-specific Duplex
DNA Binding Protein
,, and
Molecular Biophysics and
Biochemistry and Genetics, Yale University,
New Haven, Connecticut 06520-8114
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Lee, A. Gyorgy, D. Maric, N. Sadri, R. J. Schneider, J. L. Barker, M. Lawson, and D. V. Agoston Members of the NuRD Chromatin Remodeling Complex Interact with AUF1 in Developing Cortical Neurons Cereb Cortex, December 1, 2008; 18(12): 2909 - 2919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Witkowski, M. Soroczynska-Cybula, E. Bryl, Z. Smolenska, and A. Jozwik Klotho--a Common Link in Physiological and Rheumatoid Arthritis-Related Aging of Human CD4+ Lymphocytes J. Immunol., January 15, 2007; 178(2): 771 - 777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Banihashemi, G. M. Wilson, N. Das, and G. Brewer Upf1/Upf2 Regulation of 3' Untranslated Region Splice Variants of AUF1 Links Nonsense-Mediated and A+U-Rich Element-Mediated mRNA Decay Mol. Cell. Biol., December 1, 2006; 26(23): 8743 - 8754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dobi, M. Szemes, C. Lee, M. Palkovits, F. Lim, A. Gyorgy, M. A. Mahan, and D. V. Agoston AUF1 Is Expressed in the Developing Brain, Binds to AT-rich Double-stranded DNA, and Regulates Enkephalin Gene Expression J. Biol. Chem., September 29, 2006; 281(39): 28889 - 28900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Huddleson, N. Ahmad, and J. B. Lingrel Up-regulation of the KLF2 Transcription Factor by Fluid Shear Stress Requires Nucleolin J. Biol. Chem., June 2, 2006; 281(22): 15121 - 15128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gao, H. Guo, Z. Mi, P. Y. Wai, and P. C. Kuo Transcriptional Regulatory Functions of Heterogeneous Nuclear Ribonucleoprotein-U and -A/B in Endotoxin-Mediated Macrophage Expression of Osteopontin J. Immunol., July 1, 2005; 175(1): 523 - 530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Huddleson, N. Ahmad, S. Srinivasan, and J. B Lingrel Induction of KLF2 by Fluid Shear Stress Requires a Novel Promoter Element Activated by a Phosphatidylinositol 3-Kinase-dependent Chromatin-remodeling Pathway J. Biol. Chem., June 17, 2005; 280(24): 23371 - 23379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Alex and K. A. W. Lee RGG-boxes of the EWS oncoprotein repress a range of transcriptional activation domains Nucleic Acids Res., March 2, 2005; 33(4): 1323 - 1331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Saez-Vasquez, D. Caparros-Ruiz, F. Barneche, and M. Echeverria A Plant snoRNP Complex Containing snoRNAs, Fibrillarin, and Nucleolin-Like Proteins Is Competent for both rRNA Gene Binding and Pre-rRNA Processing In Vitro Mol. Cell. Biol., August 15, 2004; 24(16): 7284 - 7297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Singh, J. Laughlin, P. A. Kosinski, and L. R. Covey Nucleolin Is a Second Component of the CD154 mRNA Stability Complex That Regulates mRNA Turnover in Activated T Cells J. Immunol., July 15, 2004; 173(2): 976 - 985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Cook, H. E. Mischo, and J. A. Steitz The Herpesvirus saimiri Small Nuclear RNAs Recruit AU-Rich Element-Binding Proteins but Do Not Alter Host AU-Rich Element-Containing mRNA Levels in Virally Transformed T Cells Mol. Cell. Biol., May 15, 2004; 24(10): 4522 - 4533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gao, H. Guo, J. Wei, Z. Mi, P. Wai, and P. C. Kuo S-Nitrosylation of Heterogeneous Nuclear Ribonucleoprotein A/B Regulates Osteopontin Transcription in Endotoxin-stimulated Murine Macrophages J. Biol. Chem., March 19, 2004; 279(12): 11236 - 11243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Wilson, J. Lu, K. Sutphen, Y. Sun, Y. Huynh, and G. Brewer Regulation of A + U-rich Element-directed mRNA Turnover Involving Reversible Phosphorylation of AUF1 J. Biol. Chem., August 29, 2003; 278(35): 33029 - 33038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Saitoh, S. Miyagi, H. Ariga, and K.-i. Tsutsumi Functional domains involved in the interaction between Orc1 and transcriptional repressor AlF-C that bind to an origin/promoter of the rat aldolase B gene Nucleic Acids Res., December 1, 2002; 30(23): 5205 - 5212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Vallejo, E. Bryl, K. Klarskov, S. Naylor, C. M. Weyand, and J. J. Goronzy Molecular Basis for the Loss of CD28 Expression in Senescent T Cells J. Biol. Chem., November 27, 2002; 277(49): 46940 - 46949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zetterberg, A. Jansson, L. Rymo, F. Chen, A. Karlsson, G. Klein, and B. Brodin The Epstein-Barr virus ZEBRA protein activates transcription from the early lytic F promoter by binding to a promoter-proximal AP-1-like site J. Gen. Virol., August 1, 2002; 83(8): 2007 - 2014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Carty and A. L. Greenleaf Hyperphosphorylated C-terminal Repeat Domain-associating Proteins in the Nuclear Proteome Link Transcription to DNA/Chromatin Modification and RNA Processing Mol. Cell. Proteomics, August 1, 2002; 1(8): 598 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Laroia and R. J. Schneider Alternate exon insertion controls selective ubiquitination and degradation of different AUF1 protein isoforms Nucleic Acids Res., July 15, 2002; 30(14): 3052 - 3058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. T. Kuninger, T. Izumi, J. Papaconstantinou, and S. Mitra Human AP-endonuclease 1 and hnRNP-L interact with a nCaRE-like repressor element in the AP-endonuclease 1 promoter Nucleic Acids Res., February 1, 2002; 30(3): 823 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sun, A. Yabuki, and N. Maizels A human nuclease specific for G4 DNA PNAS, October 23, 2001; 98(22): 12444 - 12449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Monneaux, H. Dumortier, G. Steiner, J.-P. Briand, and S. Muller Murine models of systemic lupus erythematosus: B and T cell responses to spliceosomal ribonucleoproteins in MRL/Faslpr and (NZB NZW)F1 lupus mice Int. Immunol., September 1, 2001; 13(9): 1155 - 1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. K. Voeltz, J. Ongkasuwan, N. Standart, and J. A. Steitz A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts Genes & Dev., March 15, 2001; 15(6): 774 - 788. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. M. Fuentes-Pananá, R. Peng, G. Brewer, J. Tan, and P. D. Ling Regulation of the Epstein-Barr Virus C Promoter by AUF1 and the Cyclic AMP/Protein Kinase A Signaling Pathway J. Virol., September 1, 2000; 74(17): 8166 - 8175. [Abstract] [Full Text]
|
|
|
|
|
|
L. A. Hanakahi and N. Maizels Transcriptional activation by LR1 at the E{micro} enhancer and switch region sites Nucleic Acids Res., July 15, 2000; 28(14): 2651 - 2657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I.-E. Gallouzi, C. M. Brennan, M. G. Stenberg, M. S. Swanson, A. Eversole, N. Maizels, and J. A. Steitz HuR binding to cytoplasmic mRNA is perturbed by heat shock PNAS, March 28, 2000; 97(7): 3073 - 3078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Blaxall, A. C. Pellett, S. C. Wu, A. Pende, and J. D. Port Purification and Characterization of beta -Adrenergic Receptor mRNA-binding Proteins J. Biol. Chem., February 11, 2000; 275(6): 4290 - 4297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Satoh, V. M. Shaheen, P. N. Kao, T. Okano, M. Shaw, H. Yoshida, H. B. Richards, and W. H. Reeves Autoantibodies Define a Family of Proteins with Conserved Double-stranded RNA-binding Domains as Well as DNA Binding Activity J. Biol. Chem., December 3, 1999; 274(49): 34598 - 34604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Hanakahi, H. Sun, and N. Maizels High Affinity Interactions of Nucleolin with G-G-paired rDNA J. Biol. Chem., May 28, 1999; 274(22): 15908 - 15912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Dempsey, H. Sun, L. A. Hanakahi, and N. Maizels G4 DNA Binding by LR1 and Its Subunits, Nucleolin and hnRNP D, A Role for G-G pairing in Immunoglobulin Switch Recombination J. Biol. Chem., January 8, 1999; 274(2): 1066 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H Ginisty, H Sicard, B Roger, and P Bouvet Structure and functions of nucleolin J. Cell Sci., January 3, 1999; 112(6): 761 - 772. [Abstract] [PDF]
|
|
|
|
|
|
K. K. C. Li and K. A. W. Lee Transcriptional Activation by the Ewing's Sarcoma (EWS) Oncogene Can Be Cis-repressed by the EWS RNA-binding Domain J. Biol. Chem., July 21, 2000; 275(30): 23053 - 23058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-J. Jung, S.-Y. Na, D. S. Na, and J. W. Lee Molecular Cloning and Characterization of CAPER, a Novel Coactivator of Activating Protein-1 and Estrogen Receptors J. Biol. Chem., January 4, 2002; 277(2): 1229 - 1234. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
