JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suzuki, F.
Right arrow Articles by Handa, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki, F.
Right arrow Articles by Handa, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 45, 29302-29308, November 6, 1998

Functional Interactions of Transcription Factor Human GA-binding Protein Subunits

Fumihiko Suzuki, Masahide Goto, Chika Sawa, Seiichiro Ito, Hajime Watanabe, Jun-ichi Sawada, and Hiroshi Handa

From the Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan

The transcription factor human GA-binding protein (hGABP) is composed of two subunits, the Ets-related hGABPalpha , which binds to a specific DNA sequence, and either one of two hGABPalpha -associated subunits, hGABPbeta or hGABPgamma . The DNA-binding protein hGABPalpha cannot affect transcription by itself, but can modify hGABP-dependent transcription in vitro and in vivo in the presence of its associated subunits. In this study, co-transfection assays showed that the ratio of hGABPbeta to hGABPgamma affected transcription from a promoter containing hGABP binding sites. Biochemical analysis showed that they bind to hGABPalpha competitively, indicating that the ratio of hGABPbeta to hGABPgamma is important for hGABP complex formation. Kinetic analysis of the protein-protein interaction using the surface plasmon resonance system showed that hGABPalpha binds to hGABPbeta or hGABPgamma with similar equilibrium constants. Kinetic analysis of the DNA-hGABP interaction showed that the binding of hGABPgamma to hGABPalpha stabilized the interaction of hGABPalpha with its DNA binding site. In addition, the kinetic analysis revealed that this was due to a slower dissociation of the protein complex from the DNA. These results suggest that hGABPalpha -associated subunits influence the DNA binding stability of hGABPalpha and regulate hGABP-mediated transcription by competing with each other.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
X. Jing, D.-M. Zhao, T. J. Waldschmidt, and H.-H. Xue
GABP{beta}2 Is Dispensible for Normal Lymphocyte Development but Moderately Affects B Cell Responses
J. Biol. Chem., September 5, 2008; 283(36): 24326 - 24333.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H.-H. Xue, X. Jing, J. Bollenbacher-Reilley, D.-M. Zhao, J. S. Haring, B. Yang, C. Liu, G. A. Bishop, J. T. Harty, and W. J. Leonard
Targeting the GA Binding Protein {beta}1L Isoform Does Not Perturb Lymphocyte Development and Function
Mol. Cell. Biol., July 1, 2008; 28(13): 4300 - 4309.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. W. H. Woo, Y. L. Siow, and K. O
Homocysteine Induces Monocyte Chemoattractant Protein-1 Expression in Hepatocytes Mediated via Activator Protein-1 Activation
J. Biol. Chem., January 18, 2008; 283(3): 1282 - 1292.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Tsuchimochi, N. Yagishita, S. Yamasaki, T. Amano, Y. Kato, K.-i. Kawahara, S. Aratani, H. Fujita, F. Ji, A. Sugiura, et al.
Identification of a Crucial Site for Synoviolin Expression
Mol. Cell. Biol., August 15, 2005; 25(16): 7344 - 7356.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
M. Kyo, T. Yamamoto, H. Motohashi, T. Kamiya, T. Kuroita, T. Tanaka, J. D. Engel, B. Kawakami, and M. Yamamoto
Evaluation of MafG interaction with Maf recognition element arrays by surface plasmon resonance imaging technique
Genes Cells, February 1, 2004; 9(2): 153 - 164.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Sunesen, M. Huchet-Dymanus, M. O. Christensen, and J.-P. Changeux
Phosphorylation-Elicited Quaternary Changes of GA Binding Protein in Transcriptional Activation
Mol. Cell. Biol., November 15, 2003; 23(22): 8008 - 8018.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Maier, R. Ostraat, S. Parenti, D. Fitzsimmons, L. J. Abraham, C. W. Garvie, and J. Hagman
Requirements for selective recruitment of Ets proteins and activation of mb-1/Ig-{alpha} gene transcription by Pax-5 (BSAP)
Nucleic Acids Res., October 1, 2003; 31(19): 5483 - 5489.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Kohl, H. K. Binz, P. Forrer, M. T. Stumpp, A. Pluckthun, and M. G. Grutter
Designed to be stable: Crystal structure of a consensus ankyrin repeat protein
PNAS, February 18, 2003; 100(4): 1700 - 1705.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. S. Bush, M. St. Coeur, K. K. Resendes, and A. G. Rosmarin
GA-binding protein (GABP) and Sp1 are required, along with retinoid receptors, to mediate retinoic acid responsiveness of CD18 (beta 2 leukocyte integrin): a novel mechanism of transcriptional regulation in myeloid cells
Blood, January 1, 2003; 101(1): 311 - 317.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Sawa, T. Yoshikawa, F. Matsuda-Suzuki, S. Delehouzee, M. Goto, H. Watanabe, J.-i. Sawada, K. Kataoka, and H. Handa
YEAF1/RYBP and YAF-2 Are Functionally Distinct Members of a Cofactor Family for the YY1 and E4TF1/hGABP Transcription Factors
J. Biol. Chem., June 14, 2002; 277(25): 22484 - 22490.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Hauck, R. G. Kaba, M. Lipp, R. Dietz, and R. von Harsdorf
Regulation of E2F1-Dependent Gene Transcription and Apoptosis by the ETS-Related Transcription Factor GABP{gamma}1
Mol. Cell. Biol., April 1, 2002; 22(7): 2147 - 2158.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Briguet and M. A. Ruegg
The Ets Transcription Factor GABP Is Required for Postsynaptic Differentiation In Vivo
J. Neurosci., August 15, 2000; 20(16): 5989 - 5996.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Chinenov, M. Henzl, and M. E. Martin
The alpha and beta Subunits of the GA-binding Protein Form a Stable Heterodimer in Solution. REVISED MODEL OF HETEROTETRAMERIC COMPLEX ASSEMBLY
J. Biol. Chem., March 10, 2000; 275(11): 7749 - 7756.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-i. Sawada, N. Simizu, F. Suzuki, C. Sawa, M. Goto, M. Hasegawa, T. Imai, H. Watanabe, and H. Handa
Synergistic Transcriptional Activation by hGABP and Select Members of the Activation Transcription Factor/cAMP Response Element-binding Protein Family
J. Biol. Chem., December 10, 1999; 274(50): 35475 - 35482.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Gong, L. J. Brown, and M. J. MacDonald
Functional Analysis of Two Promoters for the Human Mitochondrial Glycerol Phosphate Dehydrogenase Gene
J. Biol. Chem., November 22, 2000; 275(48): 38012 - 38021.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.