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J Biol Chem, Vol. 273, Issue 45, 29341-29351, November 6, 1998

Cysteine Scanning Mutagenesis of the Segment between Putative Transmembrane Helices IV and V of the High Affinity Na⁺/Glucose Cotransporter SGLT1
EVIDENCE THAT THIS REGION PARTICIPATES IN THE Na⁺ AND VOLTAGE DEPENDENCE OF THE TRANSPORTER

From the Department of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada

Site-directed mutagenesis and chemical modification of specific cysteine amino acid side chains by methanethiosulfonate (MTS) derivatives were combined to elucidate structure/function relationships of the cloned rabbit Na⁺/glucose cotransporter, SGLT1. Each amino acid in the region (residues 162-173) between putative transmembrane helices IV and V of SGLT1 was replaced individually with Cys. Mutant proteins were expressed in Xenopus laevis oocytes and studied using the two-electrode voltage clamp method. At certain key positions, Cys substitution resulted in 1) a change in the apparent affinity for sugar, 2) an alteration in the voltage dependence of the transient currents, and 3) a sensitivity to inhibition by either the ethylamine (MTSEA) or the ethylsulfonate MTS derivatives. For the three Cys mutants inhibited by MTSEA (F163C, A166C, and L173C), inhibition of steady state transport is related to changes in membrane potential-dependent transitions within the Na⁺/glucose transport cycle. MTSEA shifted the transient currents of these Cys mutants toward more negative membrane potentials (V_0.5 = 18 mV for F163C and A166C, 12 mV for L173C). When the mutations were combined to produce double and triple Cys mutants, the degree to which the transient currents were shifted along the membrane potential axis by MTSEA correlated with the number of cysteines. In this way it was possible to manipulate the voltage dependence of the transient currents over a range spanning 91 mV. Examination of the Na⁺ dependence of the transient currents indicates that a 91-mV shift is equivalent to that caused by a 10-fold reduction in the external Na⁺ concentration. We conclude that this region has a role in determining the Na⁺ binding- and voltage-sensing properties of SGLT1 and that it forms an -helix with one surface possibly lining a Na⁺ pore within SGLT1.

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