| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 45, 29497-29505, November 6, 1998
-GLUCOSYLTRANSFERASE PROVIDES A NOVEL
ATTACHMENT SITE FOR O-POLYSACCHARIDES
From the Department of Microbiology, University of Guelph,
Guelph, Ontario N1G 2W1, Canada
The major core oligosaccharide biosynthesis
operons from prototype Escherichia coli strains displaying
R1 and R4 lipopolysaccharide core types were polymerase chain
reaction-amplified and analyzed. Comparison of deduced products of the
open reading frames between the two regions indicate that all but two
share total similarities of 94% or greater. Core oligosaccharide
structures resulting from nonpolar insertion mutations in each gene of
the core OS biosynthesis operon in the R1 strain allowed assignment of
all of the glycosyltransferase enzymes required for outer core
assembly. The difference between the R1 and R4 core oligosaccharides
results from the specificity of the WaaV protein (a
1,3-glucosyltransferase) in R1 and WaaX (a
1,4-galactosyltransferase) in R4. Complementation of the
waaV mutant of the R1 prototype strain with the
waaX gene of the R4 strain converted the core
oligosaccharide from an R1- to an R4-type lipopolysaccharide core
molecule. Aside from generating core oligosaccharide specificity, the
unique -linked glucopyranosyl residue of the R1 core plays a crucial
role in organization of the lipopolysaccharide. This residue provides a
novel attachment site for lipid A-core-linked polysaccharides and
distinguishes the R1-type LPS from existing models for enterobacterial lipopolysaccharides.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. D. Leipold, E. Vinogradov, and C. Whitfield Glycosyltransferases Involved in Biosynthesis of the Outer Core Region of Escherichia coli Lipopolysaccharides Exhibit Broader Substrate Specificities Than Is Predicted from Lipopolysaccharide Structures J. Biol. Chem., September 14, 2007; 282(37): 26786 - 26792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Spitzer, L. M. Mitchell, J. P. Atkinson, and D. E. Hourcade Properdin Can Initiate Complement Activation by Binding Specific Target Surfaces and Providing a Platform for De Novo Convertase Assembly J. Immunol., August 15, 2007; 179(4): 2600 - 2608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Zhu, R. A. Boykins, and C.-M. Tsai Genetic and functional analyses of the lgtH gene, a member of the {beta}-1,4-galactosyltransferase gene family in the genus Neisseria Microbiology, January 1, 2006; 152(1): 123 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Agladze, X. Wang, and T. Romeo Spatial Periodicity of Escherichia coli K-12 Biofilm Microstructure Initiates during a Reversible, Polar Attachment Phase of Development and Requires the Polysaccharide Adhesin PGA J. Bacteriol., December 15, 2005; 187(24): 8237 - 8246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Abeyrathne, C. Daniels, K. K. H. Poon, M. J. Matewish, and J. S. Lam Functional Characterization of WaaL, a Ligase Associated with Linking O-Antigen Polysaccharide to the Core of Pseudomonas aeruginosa Lipopolysaccharide J. Bacteriol., May 1, 2005; 187(9): 3002 - 3012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Kaniuk, E. Vinogradov, and C. Whitfield Investigation of the Structural Requirements in the Lipopolysaccharide Core Acceptor for Ligation of O Antigens in the Genus Salmonella: WAAL "LIGASE" IS NOT THE SOLE DETERMINANT OF ACCEPTOR SPECIFICITY J. Biol. Chem., August 27, 2004; 279(35): 36470 - 36480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Kaniuk, E. Vinogradov, J. Li, M. A. Monteiro, and C. Whitfield Chromosomal and Plasmid-encoded Enzymes Are Required for Assembly of the R3-type Core Oligosaccharide in the Lipopolysaccharide of Escherichia coli O157:H7 J. Biol. Chem., July 23, 2004; 279(30): 31237 - 31250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Frirdich, E. Vinogradov, and C. Whitfield Biosynthesis of a Novel 3-Deoxy-D-manno-oct-2-ulosonic Acid-containing Outer Core Oligosaccharide in the Lipopolysaccharide of Klebsiella pneumoniae J. Biol. Chem., July 2, 2004; 279(27): 27928 - 27940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Whitfield, N. Kaniuk, and E. Frirdich Molecular insights into the assembly and diversity of the outer core oligosaccharide in lipopolysaccharides from Escherichia coli and Salmonella Innate Immunity, August 1, 2003; 9(4): 244 - 249. [Abstract] [PDF]
|
|
|
|
|
|
M. L. Lawrence, M. M. Banes, P. Azadi, and B. Y. Reeks The Edwardsiella ictaluri O polysaccharide biosynthesis gene cluster and the role of O polysaccharide in resistance to normal catfish serum and catfish neutrophils Microbiology, June 1, 2003; 149(6): 1409 - 1421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Frirdich, B. Lindner, O. Holst, and C. Whitfield Overexpression of the waaZ Gene Leads to Modification of the Structure of the Inner Core Region of Escherichia coli Lipopolysaccharide, Truncation of the Outer Core, and Reduction of the Amount of O Polysaccharide on the Cell Surface J. Bacteriol., March 1, 2003; 185(5): 1659 - 1671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Vinogradov, E. Frirdich, L. L. MacLean, M. B. Perry, B. O. Petersen, J. O. Duus, and C. Whitfield Structures of Lipopolysaccharides from Klebsiella pneumoniae. ELUCIDATION OF THE STRUCTURE OF THE LINKAGE REGION BETWEEN CORE AND POLYSACCHARIDE O CHAIN AND IDENTIFICATION OF THE RESIDUES AT THE NON-REDUCING TERMINI OF THE O CHAINS J. Biol. Chem., July 5, 2002; 277(28): 25070 - 25081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Hinnebusch, A. E. Rudolph, P. Cherepanov, J. E. Dixon, T. G. Schwan, and A. Forsberg Role of Yersinia Murine Toxin in Survival of Yersinia pestis in the Midgut of the Flea Vector Science, April 26, 2002; 296(5568): 733 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Paton, T. J. Rogers, R. Morona, and A. W. Paton Oral Administration of Formaldehyde-Killed Recombinant Bacteria Expressing a Mimic of the Shiga Toxin Receptor Protects Mice from Fatal Challenge with Shiga-Toxigenic Escherichia coli Infect. Immun., March 1, 2001; 69(3): 1389 - 1393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Paton, R. Morona, and J. C. Paton Neutralization of Shiga Toxins Stx1, Stx2c, and Stx2e by Recombinant Bacteria Expressing Mimics of Globotriose and Globotetraose Infect. Immun., March 1, 2001; 69(3): 1967 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Yethon, E. Vinogradov, M. B. Perry, and C. Whitfield Mutation of the Lipopolysaccharide Core Glycosyltransferase Encoded by waaG Destabilizes the Outer Membrane of Escherichia coli by Interfering with Core Phosphorylation J. Bacteriol., October 1, 2000; 182(19): 5620 - 5623. [Abstract] [Full Text]
|
|
|
|
|
|
H. Yang, M. Matewish, I. Loubens, D. G. Storey, J. S. Lam, and S. Jin migA, a quorum-responsive gene of Pseudomonas aeruginosa, is highly expressed in the cystic fibrosis lung environment and modifies low-molecular-mass lipopolysaccharide Microbiology, October 1, 2000; 146(10): 2509 - 2519. [Abstract] [Full Text]
|
|
|
|
|
|
K. Amor, D. E. Heinrichs, E. Frirdich, K. Ziebell, R. P. Johnson, and C. Whitfield Distribution of Core Oligosaccharide Types in Lipopolysaccharides from Escherichia coli Infect. Immun., March 1, 2000; 68(3): 1116 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Whitfield, D. E. Heinrichs, J. A. Yethon, K. L. Amor, M. A. Monteiro, and M. B. Perry Assembly of the R1-type core oligosaccharide of Escherichia coli lipopolysaccharide Innate Immunity, June 1, 1999; 5(3): 151 - 156. [Abstract] [PDF]
|
|
|
|
|
|
P. A. Amor, J. A. Yethon, M. A. Monteiro, and C. Whitfield Assembly of the K40 Antigen in Escherichia coli: Identification of a Novel Enzyme Responsible for Addition of L-Serine Residues to the Glycan Backbone and Its Requirement for K40 Polymerization J. Bacteriol., February 1, 1999; 181(3): 772 - 780. [Abstract] [Full Text]
|
|
|
|
|
|
J. A. Yethon and C. Whitfield Purification and Characterization of WaaP from Escherichia coli, a Lipopolysaccharide Kinase Essential for Outer Membrane Stability J. Biol. Chem., February 16, 2001; 276(8): 5498 - 5504. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
