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J Biol Chem, Vol. 273, Issue 45, 29929-29934, November 6, 1998

Suppression of leu-500 Mutation in topA⁺ Salmonella typhimurium Strains
THE PROMOTER RELAY AT WORK

From the Department of Pharmacology, School of Medicine, Wayne State University, Detroit, Michigan 48201

Suppression of leu-500 mutation in Salmonella typhimurium topA strains has been one of the most fascinating examples for the DNA supercoiling effect on transcription initiation control. Previous studies have indicated possible involvement of transcription-driven DNA supercoiling in the activation of the leu-500 promoter in topA strains. Our recent studies have shown that ilvIH transcription activity located 1.9 kilobase pairs upstream is the initial supercoiling signal for leu-500 activation via a promoter relay mechanism. In the present communication, we show that the ilvIH transcription activity-initiated promoter relay can result in leu-500 activation in topA⁺ strains. In addition, suppression of the chromosomal leu-500 mutation correlates with the transcription activities of ilvIH and leuO rather than the TopA level in the topA⁺ strain. It appears that the leu-500 suppression in a topA strain is due to the constant ilvIH transcription activity.

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