| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 46, 30069-30072, November 13, 1998
,,
**, and**
From the Poly(ADP-ribose) polymerase (PARP) (EC 2.4.2.30),
the only enzyme known to synthesize ADP-ribose polymers from
NAD+, is activated in response to DNA strand breaks
and functions in the maintenance of genomic integrity. Mice homozygous
for a disrupted gene encoding PARP are viable but have severe
sensitivity to Department of Clinical Sciences,
Lucille P. Markey Cancer Center, § College of
Pharmacy, and ** Advanced Science and Technology Commercialization
Center, University of Kentucky, Lexington, Kentucky 40506-0286 and
¶ International Agency for Research on Cancer (IARC), Unit of
Gene Environment Interaction, IARC, 150, cours Albert-Thomas,
F-69372 Cedex 08, Lyon, France
-radiation and alkylating agents. We demonstrate here
that both 3T3 and primary embryo cells derived from
PARP
/ mice synthesized ADP-ribose polymers following
treatment with the DNA-damaging agent,
N-methyl-N'-nitro-N-nitrosoguanidine, despite the fact that no PARP protein was detected in these cells. ADP-ribose polymers isolated from PARP/ cells were
indistinguishable from that of PARP+/+ cells by several
criteria. First, they bound to a boronate resin selective for
ADP-ribose polymers. Second, treatment of polymers with snake venom
phosphodiesterase and alkaline phosphatase yielded ribosyladenosine, a
nucleoside diagnostic for the unique ribosyl-ribosyl linkages of
ADP-ribose polymers. Third, they were digested by treatment with
recombinant poly(ADP-ribose) glycohydrolase, an enzyme highly specific
for ADP-ribose polymers. Collectively, these data demonstrate that
ADP-ribose polymers are formed in PARP/ cells in a DNA
damage-dependent manner. Because the PARP gene has been
disrupted, these results suggest the presence of a previously unreported activity capable of synthesizing ADP-ribose polymers in
PARP/ cells.
This article has been cited by other articles:
|
|
 |
|
  O. A. Olabisi, N. Soto-Nieves, E. Nieves, T. T. C. Yang, X. Yang, R. Y. L. Yu, H. Y. Suk, F. Macian, and C.-W. Chow Regulation of Transcription Factor NFAT by ADP-Ribosylation Mol. Cell. Biol., May 1, 2008; 28(9): 2860 - 2871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Beneke and A. Burkle Poly(ADP-ribosyl)ation in mammalian ageing Nucleic Acids Res., December 3, 2007; 35(22): 7456 - 7465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. O. Fisher, H. Hochegger, S. Takeda, and K. W. Caldecott Poly(ADP-Ribose) Polymerase 1 Accelerates Single-Strand Break Repair in Concert with Poly(ADP-Ribose) Glycohydrolase Mol. Cell. Biol., August 1, 2007; 27(15): 5597 - 5605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Berger, C. Lau, and M. Ziegler Regulation of poly(ADP-ribose) polymerase 1 activity by the phosphorylation state of the nuclear NAD biosynthetic enzyme NMN adenylyl transferase 1 PNAS, March 6, 2007; 104(10): 3765 - 3770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. O. Hassa, S. S. Haenni, M. Elser, and M. O. Hottiger Nuclear ADP-Ribosylation Reactions in Mammalian Cells: Where Are We Today and Where Are We Going? Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 789 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Cipriani, E. Rapizzi, A. Vannacci, R. Rizzuto, F. Moroni, and A. Chiarugi Nuclear Poly(ADP-ribose) Polymerase-1 Rapidly Triggers Mitochondrial Dysfunction J. Biol. Chem., April 29, 2005; 280(17): 17227 - 17234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Beneke and A. Burkle Poly(ADP-Ribosyl)ation, PARP, and Aging Sci. Aging Knowl. Environ., December 8, 2004; 2004(49): re9 - re9. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, S.-W. Yu, D. W. Koh, J. Lew, C. Coombs, W. Bowers, H. J. Federoff, G. G. Poirier, T. M. Dawson, and V. L. Dawson Apoptosis-Inducing Factor Substitutes for Caspase Executioners in NMDA-Triggered Excitotoxic Neuronal Death J. Neurosci., December 1, 2004; 24(48): 10963 - 10973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Suzuki, E. Masini, C. Mazzocca, S. Cuzzocrea, A. Ciampa, H. Suzuki, and D. Bani Inhibition of Poly(ADP-Ribose) Polymerase Prevents Allergen-Induced Asthma-Like Reaction in Sensitized Guinea Pigs J. Pharmacol. Exp. Ther., December 1, 2004; 311(3): 1241 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nakajima, H. Nagaso, N. Kakui, M. Ishikawa, T. Hiranuma, and S. Hoshiko Critical Role of the Automodification of Poly(ADP-ribose) Polymerase-1 in Nuclear Factor-{kappa}B-dependent Gene Expression in Primary Cultured Mouse Glial Cells J. Biol. Chem., October 8, 2004; 279(41): 42774 - 42786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. C. Yung, S. Sato, and M. S. Satoh Poly(ADP-ribosyl)ation as a DNA Damage-induced Post-translational Modification Regulating Poly(ADP-ribose) Polymerase-1-Topoisomerase I Interaction J. Biol. Chem., September 17, 2004; 279(38): 39686 - 39696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. von Kobbe, J. A. Harrigan, V. Schreiber, P. Stiegler, J. Piotrowski, L. Dawut, and V. A. Bohr Poly(ADP-ribose) polymerase 1 regulates both the exonuclease and helicase activities of the Werner syndrome protein Nucleic Acids Res., August 3, 2004; 32(13): 4003 - 4014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ertsey, C. J. Chapin, J. A. Kitterman, and L. M. Scavo Ontogeny of Poly(ADP-Ribose) Polymerase-1 in Lung and Developmental Implications Am. J. Respir. Cell Mol. Biol., June 1, 2004; 30(6): 853 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Brabeck, R. Pfeiffer, A. Leake, S. Beneke, R. Meyer, and A. Burkle L-Selegiline Potentiates the Cellular Poly(ADP-Ribosyl)ation Response to Ionizing Radiation J. Pharmacol. Exp. Ther., September 1, 2003; 306(3): 973 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wieler, J.-P. Gagne, H. Vaziri, G. G. Poirier, and S. Benchimol Poly(ADP-ribose) Polymerase-1 Is a Positive Regulator of the p53-mediated G1 Arrest Response following Ionizing Radiation J. Biol. Chem., May 23, 2003; 278(21): 18914 - 18921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Siva and F. Bushman Poly(ADP-Ribose) Polymerase 1 Is Not Strictly Required for Infection of Murine Cells by Retroviruses J. Virol., October 25, 2002; 76(23): 11904 - 11910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Saxena, L. H. Wong, P. Kalitsis, E. Earle, L. G. Shaffer, and K.H. A. Choo Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc Hum. Mol. Genet., September 15, 2002; 11(19): 2319 - 2329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Virag and C. Szabo The Therapeutic Potential of Poly(ADP-Ribose) Polymerase Inhibitors Pharmacol. Rev., September 1, 2002; 54(3): 375 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Saxena, R. Saffery, L. H. Wong, P. Kalitsis, and K. H. A. Choo Centromere Proteins Cenpa, Cenpb, and Bub3 Interact with Poly(ADP-ribose) Polymerase-1 Protein and Are Poly(ADP-ribosyl)ated J. Biol. Chem., July 19, 2002; 277(30): 26921 - 26926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Schreiber, J.-C. Ame, P. Dolle, I. Schultz, B. Rinaldi, V. Fraulob, J. Menissier-de Murcia, and G. de Murcia Poly(ADP-ribose) Polymerase-2 (PARP-2) Is Required for Efficient Base Excision DNA Repair in Association with PARP-1 and XRCC1 J. Biol. Chem., June 14, 2002; 277(25): 23028 - 23036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gonzalez, J. Menissier de Murcia, P. Janiak, J.-P. Bidouard, C. Beauvais, S. Karray, H.-J. Garchon, and M. Levi-Strauss Unexpected Sensitivity of Nonobese Diabetic Mice With a Disrupted Poly(ADP-Ribose) Polymerase-1 Gene to Streptozotocin-Induced and Spontaneous Diabetes Diabetes, May 1, 2002; 51(5): 1470 - 1476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Los, M. Mozoluk, D. Ferrari, A. Stepczynska, C. Stroh, A. Renz, Z. Herceg, Z.-Q. Wang, and K. Schulze-Osthoff Activation and Caspase-mediated Inhibition of PARP: A Molecular Switch between Fibroblast Necrosis and Apoptosis in Death Receptor Signaling Mol. Biol. Cell, March 1, 2002; 13(3): 978 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Ha, L. D. Hester, and S. H. Snyder Poly(ADP-ribose) polymerase-1 dependence of stress-induced transcription factors and associated gene expression in glia PNAS, February 14, 2002; (2002) 52712399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bakondi, P. Bai, E. Szabo, J. Hunyadi, P. Gergely, C. Szabo, and L. Virag Detection of Poly(ADP-ribose) Polymerase Activation in Oxidatively Stressed Cells and Tissues Using Biotinylated NAD Substrate J. Histochem. Cytochem., January 1, 2002; 50(1): 91 - 98. [Abstract] [Full Text]
|
|
|
|
 |
|
 K.P. Guruprasad, V. Vasudev, M.N. Anilkumar, and S.A. Chethan Inducible protective processes in animal systems. X. Influence of nicotinamide in methyl methanesulfonate-adapted mouse bone marrow cells Mutagenesis, January 1, 2002; 17(1): 1 - 8. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Affar, R. G. Shah, A.-K. Dallaire, V. Castonguay, and G. M. Shah Role of poly(ADP-ribose) polymerase in rapid intracellular acidification induced by alkylating DNA damage PNAS, December 21, 2001; (2001) 12460399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.P. Guruprasad and V. Vasudev Inducible protective processes in animal systems: VIII. Enhancement of adaptive response by nicotinamide Mutagenesis, May 1, 2001; 16(3): 257 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Simbulan-Rosenthal, D. S. Rosenthal, R. Luo, J.-H. Li, J. Zhang, and M. E. Smulson Inhibition of poly(ADP-ribose) polymerase activity is insufficient to induce tetraploidy Nucleic Acids Res., February 1, 2001; 29(3): 841 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tsutsumi, M. Masutani, T. Nozaki, O. Kusuoka, T. Tsujiuchi, H. Nakagama, H. Suzuki, Y. Konishi, and T. Sugimura Increased susceptibility of poly(ADP-ribose) polymerase-1 knockout mice to nitrosamine carcinogenicity Carcinogenesis, January 1, 2001; 22(1): 1 - 3. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. B. Jones, J. McIntosh, H. Huang, A. Graytock, and D. G. Hoyt Regulation of Bleomycin-Induced DNA Breakage and Chromatin Structure in Lung Endothelial Cells by Integrins and Poly(ADP-Ribose) Polymerase Mol. Pharmacol., January 1, 2001; 59(1): 69 - 75. [Abstract] [Full Text]
|
|
|
|
|
|
M. D. Vodenicharov, F. R. Sallmann, M. S. Satoh, and G. G. Poirier Base excision repair is efficient in cells lacking poly(ADP-ribose) polymerase 1 Nucleic Acids Res., October 15, 2000; 28(20): 3887 - 3896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Simbulan-Rosenthal, D. H. Ly, D. S. Rosenthal, G. Konopka, R. Luo, Z.-Q. Wang, P. G. Schultz, and M. E. Smulson Misregulation of gene expression in primary fibroblasts lacking poly(ADP-ribose) polymerase PNAS, September 29, 2000; (2000) 200285797. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. R. Pettitt, P. D. Sherrington, and J. C. Cawley Role of Poly(ADP-ribosyl)ation in the Killing of Chronic Lymphocytic Leukemia Cells by Purine Analogues Cancer Res., August 1, 2000; 60(15): 4187 - 4193. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. BURKLE Poly(ADP-Ribosyl)ation, Genomic Instability, and Longevity Ann. N.Y. Acad. Sci., June 1, 2000; 908(1): 126 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Sallmann, M. D. Vodenicharov, Z.-Q. Wang, and G. G. Poirier Characterization of sPARP-1. AN ALTERNATIVE PRODUCT OF PARP-1 GENE WITH POLY(ADP-RIBOSE) POLYMERASE ACTIVITY INDEPENDENT OF DNA STRAND BREAKS J. Biol. Chem., May 12, 2000; 275(20): 15504 - 15511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Pieper, S. Blackshaw, E. E. Clements, D. J. Brat, D. K. Krug, A. J. White, P. Pinto-Garcia, A. Favit, J. R. Conover, S. H. Snyder, et al. Poly(ADP-ribosyl)ation basally activated by DNA strand breaks reflects glutamate-nitric oxide neurotransmission PNAS, February 15, 2000; 97(4): 1845 - 1850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Halappanavar, Y. L. Rhun, S. Mounir, L. M. Martins, J. Huot, W. C. Earnshaw, and G. M. Shah Survival and Proliferation of Cells Expressing Caspase-uncleavable Poly(ADP-ribose) Polymerase in Response to Death-inducing DNA Damage by an Alkylating Agent J. Biol. Chem., December 24, 1999; 274(52): 37097 - 37104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Simbulan-Rosenthal, B. R. Haddad, D. S. Rosenthal, Z. Weaver, A. Coleman, R. Luo, H. M. Young, Z.-Q. Wang, T. Ried, and M. E. Smulson Chromosomal aberrations in PARP-/- mice: Genome stabilization in immortalized cells by reintroduction of poly(ADP-ribose) polymerase cDNA PNAS, November 9, 1999; 96(23): 13191 - 13196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nozaki, M. Masutani, M. Watanabe, T. Ochiya, F. Hasegawa, H. Nakagama, H. Suzuki, and T. Sugimura Syncytiotrophoblastic giant cells in teratocarcinoma-like tumors derived from Parp-disrupted mouse embryonic stem cells PNAS, November 9, 1999; 96(23): 13345 - 13350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Germain, E. B. Affar, D. D'Amours, V. M. Dixit, G. S. Salvesen, and G. G. Poirier Cleavage of Automodified Poly(ADP-ribose) Polymerase during Apoptosis. EVIDENCE FOR INVOLVEMENT OF CASPASE-7 J. Biol. Chem., October 1, 1999; 274(40): 28379 - 28384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A. Kickhoefer, A. C. Siva, N. L. Kedersha, E. M. Inman, C. Ruland, M. Streuli, and L. H. Rome The 193-kD Vault Protein, VPARP, Is a Novel Poly(ADP-ribose) Polymerase J. Cell Biol., September 6, 1999; 146(5): 917 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Ame, V. Rolli, V. Schreiber, C. Niedergang, F. Apiou, P. Decker, S. Muller, T. Hoger, J. M.-d. Murcia, and G. de Murcia PARP-2, A Novel Mammalian DNA Damage-dependent Poly(ADP-ribose) Polymerase J. Biol. Chem., June 18, 1999; 274(25): 17860 - 17868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Wu, T. F. Burns, Y. Zhan, E. S. Alnemri, and W. S. El-Deiry Molecular Cloning and Functional Analysis of the Mouse Homologue of the KILLER/DR5 Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL) Death Receptor Cancer Res., June 1, 1999; 59(12): 2770 - 2775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Pieper, D. J. Brat, D. K. Krug, C. C. Watkins, A. Gupta, S. Blackshaw, A. Verma, Z.-Q. Wang, and S. H. Snyder Poly(ADP-ribose) polymerase-deficient mice are protected from streptozotocin-induced diabetes PNAS, March 16, 1999; 96(6): 3059 - 3064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Affar, M. Germain, E. Winstall, M. Vodenicharov, R. G. Shah, G. S. Salvesen, and G. G. Poirier Caspase-3-mediated Processing of Poly(ADP-ribose) Glycohydrolase during Apoptosis J. Biol. Chem., January 19, 2001; 276(4): 2935 - 2942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Kaminker, S.-H. Kim, R. D. Taylor, Y. Zebarjadian, W. D. Funk, G. B. Morin, P. Yaswen, and J. Campisi TANK2, a New TRF1-associated Poly(ADP-ribose) Polymerase, Causes Rapid Induction of Cell Death upon Overexpression J. Biol. Chem., September 14, 2001; 276(38): 35891 - 35899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Affar, R. G. Shah, A.-K. Dallaire, V. Castonguay, and G. M. Shah Role of poly(ADP-ribose) polymerase in rapid intracellular acidification induced by alkylating DNA damage PNAS, January 8, 2002; 99(1): 245 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Ha, L. D. Hester, and S. H. Snyder Poly(ADP-ribose) polymerase-1 dependence of stress-induced transcription factors and associated gene expression in glia PNAS, March 5, 2002; 99(5): 3270 - 3275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Simbulan-Rosenthal, D. H. Ly, D. S. Rosenthal, G. Konopka, R. Luo, Z.-Q. Wang, P. G. Schultz, and M. E. Smulson Misregulation of gene expression in primary fibroblasts lacking poly(ADP-ribose) polymerase PNAS, October 10, 2000; 97(21): 11274 - 11279. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
