| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 46, 30379-30390, November 13, 1998
From the We describe novel biochemical and electron
microscopy assays to investigate in vitro fusion of latex
bead phagosomes with three different endocytic organelle fractions from
J774 macrophages. After formation, early phagosomes fuse avidly with
early and late endosomes and for a longer period of time with
lysosomes, but they subsequently become fusion-incompetent. The fusion
of early, but not late, phagosomes with all three endocytic fractions
could be significantly stimulated by Rab5. In contrast to other cell types investigated, this Rab is uniquely enriched on both early and
late endosomes in J774 macrophages. Moreover, exogenous Rab5 stimulates
homotypic fusion between both sets of organelles. This was shown by a
quantitative electron microscopy fusion assay that can directly assay
fusion between any combination of morphologically defined organelles.
By the same approach, we discovered an unexpected Rab5-stimulatable
fusion between early and late endosomes in J774, but not in BHK cells.
Thus, in J774 cells both Rab5 and the endocytic pathway seem to have
evolved additional functions not yet seen in nonphagocytic cells.
In Vitro Fusion of Phagosomes with Different
Endocytic Organelles from J774 Macrophages
,,
,
Cell Biology Programme, European Molecular
Biology Laboratory, Postfach 10.2209, D-69012 Heidelberg, Germany, the
¶ University of Oslo, MCB, P.O. Box 1050, Blindern, 0136 Oslo,
Norway, the Department of Biochemistry, Virginia Tech,
Blacksburg, Virginia 24061-0308, and ** Institut für Biochemie,
Universität Mainz, Becherweg 30, D-55128 Mainz, Germany
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. D. Rogers and L. J. Foster The dynamic phagosomal proteome and the contribution of the endoplasmic reticulum PNAS, November 20, 2007; 104(47): 18520 - 18525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Trivedi, S. C. Zhang, W. Stockinger, and A. Nohturfft A Cell-Free Scintillation Proximity Assay for Studies on Lysosome-to-Phagosome Targeting Sci. Signal., June 26, 2007; 2007(392): pl3 - pl3. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-P. Erwig, K. A. McPhilips, M. W. Wynes, A. Ivetic, A. J. Ridley, and P. M. Henson Differential regulation of phagosome maturation in macrophages and dendritic cells mediated by Rho GTPases and ezrin-radixin-moesin (ERM) proteins PNAS, August 22, 2006; 103(34): 12825 - 12830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Saito-Nakano, T. Yasuda, K. Nakada-Tsukui, M. Leippe, and T. Nozaki Rab5-associated Vacuoles Play a Unique Role in Phagocytosis of the Enteric Protozoan Parasite Entamoeba histolytica J. Biol. Chem., November 19, 2004; 279(47): 49497 - 49507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chua and V. Deretic Mycobacterium tuberculosis Reprograms Waves of Phosphatidylinositol 3-Phosphate on Phagosomal Organelles J. Biol. Chem., August 27, 2004; 279(35): 36982 - 36992. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Lucocq, A. Habermann, S. Watt, J. M. Backer, T. M. Mayhew, and G. Griffiths A Rapid Method for Assessing the Distribution of Gold Labeling on Thin Sections J. Histochem. Cytochem., August 1, 2004; 52(8): 991 - 1000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kjeken, M. Egeberg, A. Habermann, M. Kuehnel, P. Peyron, M. Floetenmeyer, P. Walther, A. Jahraus, H. Defacque, S. A. Kuznetsov, et al. Fusion between Phagosomes, Early and Late Endosomes: A Role for Actin in Fusion between Late, but Not Early Endocytic Organelles Mol. Biol. Cell, January 1, 2004; 15(1): 345 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Wang, D. Lan, M. Gerbod-Giannone, P. Linsel-Nitschke, A. W. Jehle, W. Chen, L. O. Martinez, and A. R. Tall ATP-binding Cassette Transporter A7 (ABCA7) Binds Apolipoprotein A-I and Mediates Cellular Phospholipid but Not Cholesterol Efflux J. Biol. Chem., October 31, 2003; 278(44): 42906 - 42912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. V. Vieira, C. Bucci, R. E. Harrison, W. S. Trimble, L. Lanzetti, J. Gruenberg, A. D. Schreiber, P. D. Stahl, and S. Grinstein Modulation of Rab5 and Rab7 Recruitment to Phagosomes by Phosphatidylinositol 3-Kinase Mol. Cell. Biol., April 1, 2003; 23(7): 2501 - 2514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Duclos, R. Corsini, and M. Desjardins Remodeling of endosomes during lysosome biogenesis involves `kiss and run' fusion events regulated by rab5 J. Cell Sci., March 1, 2003; 116(5): 907 - 918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Collins, A. D. Schreiber, S. Grinstein, and W. S. Trimble Syntaxins 13 and 7 Function at Distinct Steps During Phagocytosis J. Immunol., September 15, 2002; 169(6): 3250 - 3256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fratti, J. Chua, and V. Deretic Cellubrevin Alterations and Mycobacterium tuberculosis Phagosome Maturation Arrest J. Biol. Chem., May 3, 2002; 277(19): 17320 - 17326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Webster Early Intracellular Events During Internalization of Listeria monocytogenes by J774 Cells J. Histochem. Cytochem., April 1, 2002; 50(4): 503 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Al-Haddad, M. A. Shonn, B. Redlich, A. Blocker, J. K. Burkhardt, H. Yu, J. A. Hammer III, D. G. Weiss, W. Steffen, G. Griffiths, et al. Myosin Va Bound to Phagosomes Binds to F-Actin and Delays Microtubule-dependent Motility Mol. Biol. Cell, September 1, 2001; 12(9): 2742 - 2755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Fratti, J. M. Backer, J. Gruenberg, S. Corvera, and V. Deretic Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest J. Cell Biol., August 6, 2001; 154(3): 631 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Garin, R. Diez, S. Kieffer, J.-F. Dermine, S. Duclos, E. Gagnon, R. Sadoul, C. Rondeau, and M. Desjardins The Phagosome Proteome: Insight into Phagosome Functions J. Cell Biol., January 8, 2001; 152(1): 165 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rupper, B. Grove, and J. Cardelli Rab7 regulates phagosome maturation in Dictyostelium J. Cell Sci., January 7, 2001; 114(13): 2449 - 2460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Naroeni, N. Jouy, S. Ouahrani-Bettache, J.-P. Liautard, and F. Porte Brucella suis-Impaired Specific Recognition of Phagosomes by Lysosomes due to Phagosomal Membrane Modifications Infect. Immun., January 1, 2001; 69(1): 486 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jahraus, M. Egeberg, B. Hinner, A. Habermann, E. Sackman, A. Pralle, H. Faulstich, V. Rybin, H. Defacque, and G. Griffiths ATP-dependent Membrane Assembly of F-Actin Facilitates Membrane Fusion Mol. Biol. Cell, January 1, 2001; 12(1): 155 - 170. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Punturieri, S. Filippov, E. Allen, I. Caras, R. Murray, V. Reddy, and S. J. Weiss Regulation of Elastinolytic Cysteine Proteinase Activity in Normal and Cathepsin K-deficient Human Macrophages J. Exp. Med., September 11, 2000; 192(6): 789 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hashim, K. Mukherjee, M. Raje, S. K. Basu, and A. Mukhopadhyay Live Salmonella Modulate Expression of Rab Proteins to Persist in a Specialized Compartment and Escape Transport to Lysosomes J. Biol. Chem., May 19, 2000; 275(21): 16281 - 16288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mukherjee, S. A. Siddiqi, S. Hashim, M. Raje, S. K. Basu, and A. Mukhopadhyay Live Salmonella Recruits N-Ethylmaleimide-sensitive Fusion Protein on Phagosomal Membrane and Promotes Fusion with Early Endosome J. Cell Biol., February 21, 2000; 148(4): 741 - 754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Duclos, R Diez, J Garin, B Papadopoulou, A Descoteaux, H Stenmark, and M Desjardins Rab5 regulates the kiss and run fusion between phagosomes and endosomes and the acquisition of phagosome leishmanicidal properties in RAW 264.7 macrophages J. Cell Sci., January 10, 2000; 113(19): 3531 - 3541. [Abstract] [PDF]
|
|
|
|
|
|
C. Alvarez-Dominguez and P. D. Stahl Increased Expression of Rab5a Correlates Directly with Accelerated Maturation of Listeria monocytogenes Phagosomes J. Biol. Chem., April 23, 1999; 274(17): 11459 - 11462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Arora, M. F. Manolson, G. P. Downey, J. Sodek, and C. A. G. McCulloch A Novel Model System for Characterization of Phagosomal Maturation, Acidification, and Intracellular Collagen Degradation in Fibroblasts J. Biol. Chem., November 3, 2000; 275(45): 35432 - 35441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Prada-Delgado, E. Carrasco-Marin, G. M. Bokoch, and C. Alvarez-Dominguez Interferon-gamma Listericidal Action Is Mediated by Novel Rab5a Functions at the Phagosomal Environment J. Biol. Chem., May 25, 2001; 276(22): 19059 - 19065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Peyron, I. Maridonneau-Parini, and T. Stegmann Fusion of Human Neutrophil Phagosomes with Lysosomes in Vitro. INVOLVEMENT OF TYROSINE KINASES OF THE Src FAMILY AND INHIBITION BY MYCOBACTERIA J. Biol. Chem., September 14, 2001; 276(38): 35512 - 35517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Defacque, E. Bos, B. Garvalov, C. Barret, C. Roy, P. Mangeat, H.-W. Shin, V. Rybin, and G. Griffiths Phosphoinositides Regulate Membrane-dependent Actin Assembly by Latex Bead Phagosomes Mol. Biol. Cell, April 1, 2002; 13(4): 1190 - 1202. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
