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J Biol Chem, Vol. 273, Issue 46, 30757-30761, November 13, 1998

Aerobic Repression of the Rhodobacter capsulatus bchC Promoter Involves Cooperative Interactions between CrtJ Bound to Neighboring Palindromes

Stephen N. Ponnampalam, Sylvie Elsen^¶, and Carl E. Bauer^¶

From the  Biochemistry Program, Department of Chemistry and the ^¶ Department of Biology, Indiana University, Bloomington, Indiana 47405

Previous studies demonstrated that bacteriochlorophyll, carotenoid, and light harvesting gene expression in Rhodobacter capsulatus is repressed under aerobic growth conditions by the repressor CrtJ. Isolated CrtJ is known to bind to the palindrome TGTN₁₂ACA, which is present in two copies in the bchC promoter, one of which spans the 35 and the other the 10 -70 recognition sequences. In this study, we demonstrate that CrtJ binds to the two palindromic sites in the bchC promoter in a cooperative manner. The level of cooperativity of CrtJ binding to the 35 palindrome was shown to be 26-fold. A distance of 8 base pairs between the two palindromic sites was shown to be critical for cooperative binding, as evidenced by the disruption of binding that resulted when +6 and +11 base pairs were inserted between the palindromes.

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