|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 47, 31215-31221, November 20, 1998
From the Departments of The pancreas secretes several different lipases.
The most abundant is pancreatic triglyceride lipase (PTL). The pancreas
also synthesizes two homologues of PTL, the pancreatic lipase-related proteins 1 and 2 (PLRP1 and PLRP2). Cytotoxic T-lymphocytes also express PLRP2 under certain conditions. We sought to determine the role
of PLRP2 in fat absorption and in T-cell cytotoxicity by creating a
PLRP2-deficient mouse. Adult PLRP2-deficient mice had normal fat
absorption. In contrast, suckling PLRP2-deficient mice had fat
malabsorption evidenced by increased fecal weight, increased fecal
fats, and the presence of undigested and partially digested dietary
triglycerides in the feces. As a result, the PLRP2-deficient pups had a
decreased rate of weight gain. To assess T cell cytotoxicity, we
immunized PLRP2-deficient mice with a mastocytoma cell line, P815, and
determined the ability of splenocytes from the immunized mice to kill
P815 cells in a 51Cr release assay. PLRP2-deficient
cells had deficient killing activity in this assay, and PLRP2-deficient
splenocytes released fewer fatty acid from the target cells than did
control cells. Our results provide the first evidence of a
physiological function for PLRP2. PLRP2 participates in T cell
cytotoxicity, and PLRP2 performs a crucial role in the digestion of
dietary fats in suckling animals.
Decreased Neonatal Dietary Fat Absorption and T Cell Cytotoxicity
in Pancreatic Lipase-related Protein 2-Deficient Mice
§,
,,, and§§
Pediatrics and
§ Molecular Biology and Pharmacology, Washington University
School of Medicine, St. Louis, Missouri 63110, the Department of
Immunology and Infectious Diseases, Harvard School of Public Health,
Boston, Massachusetts 02115, the §§ Department
of Medicine, Harvard Medical School, Boston, Massachusetts 02115, and
The Whitehead Institute for Biomedical
Research, Cambridge, Massachusetts 02142
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Alves, J. Leong, D. L. Tamang, V. Elliott, M. Lowe, and D. Hudig Hydrolysis of tumor cell lipids after CTL-mediated death Int. Immunol., May 1, 2009; 21(5): 543 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Pang, C. Li, Y. Zhao, S. Wang, W. Dong, P. Jiang, and J. Zhang The Environmental Light Influences the Circulatory Levels of Retinoic Acid and Associates with Hepatic Lipid Metabolism Endocrinology, December 1, 2008; 149(12): 6336 - 6342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Miller and M. E. Lowe Carboxyl Ester Lipase from Either Mother's Milk or the Pancreas Is Required for Efficient Dietary Triglyceride Digestion in Suckling Mice J. Nutr., May 1, 2008; 138(5): 927 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Gilham, E. D. Labonte, J. C. Rojas, R. J. Jandacek, P. N. Howles, and D. Y. Hui Carboxyl Ester Lipase Deficiency Exacerbates Dietary Lipid Absorption Abnormalities and Resistance to Diet-induced Obesity in Pancreatic Triglyceride Lipase Knockout Mice J. Biol. Chem., August 24, 2007; 282(34): 24642 - 24649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Eydoux, J. De Caro, F. Ferrato, P. Boullanger, D. Lafont, R. Laugier, F. Carriere, and A. De Caro Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells J. Lipid Res., July 1, 2007; 48(7): 1539 - 1549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Carrier, G. Deblois, C. Champigny, E. Levy, and V. Giguere Estrogen-related Receptor {alpha} (ERR{alpha}) Is a Transcriptional Regulator of Apolipoprotein A-IV and Controls Lipid Handling in the Intestine J. Biol. Chem., December 10, 2004; 279(50): 52052 - 52058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D'Agostino and M. E. Lowe Pancreatic Lipase-related Protein 2 Is the Major Colipase-Dependent Pancreatic Lipase in Suckling Mice J. Nutr., January 1, 2004; 134(1): 132 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Huggins, L. M. Camarota, P. N. Howles, and D. Y. Hui Pancreatic Triglyceride Lipase Deficiency Minimally Affects Dietary Fat Absorption but Dramatically Decreases Dietary Cholesterol Absorption in Mice J. Biol. Chem., October 31, 2003; 278(44): 42899 - 42905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. E. Aldwell, I. G. Tucker, G. W. de Lisle, and B. M. Buddle Oral Delivery of Mycobacterium bovis BCG in a Lipid Formulation Induces Resistance to Pulmonary Tuberculosis in Mice Infect. Immun., January 1, 2003; 71(1): 101 - 108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Lowe The triglyceride lipases of the pancreas J. Lipid Res., December 1, 2002; 43(12): 2007 - 2016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Huggins, A. C. Boileau, and D. Y. Hui Protection against diet-induced obesity and obesity- related insulin resistance in Group 1B PLA2-deficient mice Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E994 - E1001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D'Agostino, R. A. Cordle, J. Kullman, C. Erlanson-Albertsson, L. J. Muglia, and M. E. Lowe Decreased Postnatal Survival and Altered Body Weight Regulation in Procolipase-deficient Mice J. Biol. Chem., February 22, 2002; 277(9): 7170 - 7177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. V. Hooper, M. H. Wong, A. Thelin, L. Hansson, P. G. Falk, and J. I. Gordon Molecular Analysis of Commensal Host-Microbial Relationships in the Intestine Science, February 2, 2001; 291(5505): 881 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. N. Howles, G. N. Stemmerman, C. M. Fenoglio-Preiser, and D. Y. Hui Carboxyl ester lipase activity in milk prevents fat-derived intestinal injury in neonatal mice Am J Physiol Gastrointest Liver Physiol, September 1, 1999; 277(3): G653 - G661. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |