HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mueller, P. P. Articles by Trachsel, H. Search for Related Content PubMed PubMed Citation Articles by Mueller, P. P. Articles by Trachsel, H. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 49, 32870-32877, December 4, 1998

A Ribosomal Protein Is Required for Translational Regulation of GCN4 mRNA
EVIDENCE FOR INVOLVEMENT OF THE RIBOSOME IN eIF2 RECYCLING

Peter P. Mueller^§¶, Patrick Grueter, Alan G. Hinnebusch^§, and Hans Trachsel

From the  Institute of Biochemistry and Molecular Biology, University of Berne, CH-3012 Berne, Switzerland, the ^§ Laboratory of Eukaryotic Gene Regulation, NICHHD, National Institutes of Health, Bethesda, Maryland 20892, and ^¶ RDIF/GBF, National Research Institute for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany

In amino acid-starved yeast cells, inhibition of the guanine nucleotide exchange factor eIF2B by phosphorylated translation initiation factor 2 results in increased translation of GCN4 mRNA. We isolated a suppressor of a mutant eIF2B. The suppressor prevents efficient GCN4 mRNA translation due to inactivation of the small ribosomal subunit protein Rps31 and results in low amounts of mutant 40 S ribosomal subunits. Deletion of one of two genes encoding ribosomal protein Rps17 also reduces the amounts of 40 S subunits but does not suppress eIF2B mutations or prevent efficient GCN4 translation. Our findings show that Rps31-deficient ribosomes are altered in a way that decreases the eIF2B requirement and that the small ribosomal subunit mediates the effects of low eIF2B activity on cell viability and translational regulation in response to eIF2 phosphorylation.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				F. L. Erickson, J. Nika, S. Rippel, and E. M. Hannig Minimum Requirements for the Function of Eukaryotic Translation Initiation Factor 2 Genetics, May 1, 2001; 158(1): 123 - 132. [Abstract] [Full Text]

				M. Hemmings-Mieszczak, T. Hohn, and T. Preiss Termination and Peptide Release at the Upstream Open Reading Frame Are Required for Downstream Translation on Synthetic Shunt-Competent mRNA Leaders Mol. Cell. Biol., September 1, 2000; 20(17): 6212 - 6223. [Abstract] [Full Text]

				M. M. Pooggin, T. Hohn, and J. Futterer Role of a Short Open Reading Frame in Ribosome Shunt on the Cauliflower Mosaic Virus RNA Leader J. Biol. Chem., June 2, 2000; 275(23): 17288 - 17296. [Abstract] [Full Text] [PDF]