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J Biol Chem, Vol. 273, Issue 5, 2489-2492, January 30, 1998

COMMUNICATION
p160 RhoA-binding Kinase ROK Induces Neurite Retraction

Hironori Katoh, Junko Aoki, Atsushi Ichikawa, and Manabu Negishi

From the Department of Physiological Chemistry and the  Department of Molecular Neurobiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606, Japan

We previously reported that the activation of prostaglandin E receptor EP3 subtype caused neurite retraction via small GTPase Rho in the EP3B receptor-expressing PC12 cells (Katoh, H., Negishi, M., and Ichikawa, A. (1996) J. Biol. Chem. 271, 29780-29784). However, a potential downstream effector of Rho that induces neurite retraction was not identified. Here we examined the morphological effect of p160 RhoA-binding kinase ROK, a target for RhoA recently identified, on the nerve growth factor-differentiated PC12 cells. Microinjection of the catalytic domain of ROK rapidly induced neurite retraction similar to that induced by microinjection of a constitutively active Rho, Rho^V14, whereas microinjection of the kinase-deficient catalytic domain of ROK did not induce neurite retraction. This morphological change was observed even though C3 exoenzyme, which was known to inactivate Rho, had been preinjected. On the other hand, microinjection of the Rho-binding domain or the pleckstrin homology domain of ROK inhibited the EP3 receptor-induced neurite retraction. These results demonstrate that ROK induces neurite retraction acting downstream of Rho in neuronal cells.

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Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.

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