HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kone, B. C. Articles by Higham, S. C. Search for Related Content PubMed PubMed Citation Articles by Kone, B. C. Articles by Higham, S. C. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 5, 2543-2552, January 30, 1998

A Novel N-terminal Splice Variant of the Rat H⁺-K⁺-ATPase 2 Subunit
CLONING, FUNCTIONAL EXPRESSION, AND RENAL ADAPTIVE RESPONSE TO CHRONIC HYPOKALEMIA

From the Departments of Internal Medicine and of Integrative Biology, Pharmacology and Physiology, The University of Texas Medical School, Houston, Texas 77030

The H⁺-K⁺-ATPase of renal collecting duct mediates K⁺ conservation during chronic hypokalemia. K⁺ deprivation promotes H⁺-K⁺-ATPase 2 (HK2) gene expression in the medullary collecting duct, the principal site of active K⁺ reabsorption, suggesting that this isozyme contributes to renal K⁺ reclamation. We report here that alternative transcriptional initiation and mRNA splicing give rise to distinct N-terminal variants of the HK2 subunit. Sequence analysis and in vitro translation revealed that HK2a corresponds to the known HK2 cDNA (Crowson, M. S., and Shull, G. E. (1992) J. Biol. Chem. 267, 13740-13748), whereas HK2b represents a novel variant truncated by 108 amino acids at its N terminus. HK2b mRNA contains a complex 5-untranslated region with eight upstream open reading frames, features implicated in translational regulation of other genes. Heterologous expression of HK2b with and without the gastric H⁺-K⁺-ATPase  subunit in HEK 293 cells indicated that this variant encodes a K⁺ uptake mechanism that is relatively Sch 28080-resistant, partially sensitive to ouabain, and appears to require coexpression with the gastric H⁺-K⁺-ATPase  subunit for optimal functional activity. Northern analysis demonstrated that both subtypes (HK2b > HK2a) are expressed abundantly in distal colon and modestly in proximal colon and kidney. Moreover, the abundance of the two mRNAs increases coordinately among the renal zones, but not in colon, with chronic K⁺ deprivation. These results demonstrate the potential for complex control of HK2 gene expression by transcriptional and posttranscriptional mechanisms not recognized in other members of the Na⁺-K⁺-ATPase/H⁺-K⁺-ATPase family.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				H. G. P. Swarts, J. B. Koenderink, P. H. G. M. Willems, and J. J. H. H. M. De Pont The Non-gastric H,K-ATPase Is Oligomycin-sensitive and Can Function as an H+,NH4+-ATPase J. Biol. Chem., September 30, 2005; 280(39): 33115 - 33122. [Abstract] [Full Text] [PDF]

				N. B. Pestov, T. V. Korneenko, R. Radkov, H. Zhao, M. I. Shakhparonov, and N. N. Modyanov Identification of the {beta}-subunit for nongastric H-K-ATPase in rat anterior prostate Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1229 - C1237. [Abstract] [Full Text] [PDF]

				W. Zhang, X. Xia, L. Zou, X. Xu, G. D. LeSage, and B. C. Kone In vivo expression profile of a H+-K+-ATPase {alpha}2-subunit promoter-reporter transgene Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1171 - F1177. [Abstract] [Full Text] [PDF]

				S. Zhang, Y. Yang, B. C. Kone, J. C. Allen, and A. M. Kahn Insulin-Stimulated Cyclic Guanosine Monophosphate Inhibits Vascular Smooth Muscle Cell Migration by Inhibiting Ca/Calmodulin-Dependent Protein Kinase II Circulation, March 25, 2003; 107(11): 1539 - 1544. [Abstract] [Full Text] [PDF]

				J. Reinhardt, M. Kosch, M. Lerner, H. Bertram, D. Lemke, and H. Oberleithner Stimulation of protein kinase C pathway mediates endocytosis of human nongastric H+-K+-ATPase, ATP1AL1 Am J Physiol Renal Physiol, August 1, 2002; 283(2): F335 - F343. [Abstract] [Full Text] [PDF]

				D. Li, H. Zhu, K. Liu, X. Liu, G. Leggewie, M. Udvardi, and D. Wang Purple Acid Phosphatases of Arabidopsis thaliana. COMPARATIVE ANALYSIS AND DIFFERENTIAL REGULATION BY PHOSPHATE DEPRIVATION J. Biol. Chem., July 26, 2002; 277(31): 27772 - 27781. [Abstract] [Full Text] [PDF]

				G. Crambert, J.-D. Horisberger, N. N. Modyanov, and K. Geering Human nongastric H+-K+-ATPase: transport properties of ATP1al1 assembled with different beta -subunits Am J Physiol Cell Physiol, July 1, 2002; 283(1): C305 - C314. [Abstract] [Full Text] [PDF]

				W. Zhang, T. Kuncewicz, S. C. Higham, and B. C. Kone Structure, Promoter Analysis, and Chromosomal Localization of the Murine H+/K+-ATPase {alpha}2 Subunit Gene J. Am. Soc. Nephrol., December 1, 2001; 12(12): 2554 - 2564. [Abstract] [Full Text] [PDF]

				J. A. Kraut, K. G. Helander, H. F. Helander, N. D. Iroezi, E. A. Marcus, and G. Sachs Detection and localization of H+-K+-ATPase isoforms in human kidney Am J Physiol Renal Physiol, October 1, 2001; 281(4): F763 - F768. [Abstract] [Full Text] [PDF]

				G. Fejes-Toth and A. Naray-Fejes-Toth Immunohistochemical localization of colonic H-K-ATPase to the apical membrane of connecting tubule cells Am J Physiol Renal Physiol, August 1, 2001; 281(2): F318 - F325. [Abstract] [Full Text] [PDF]

				T. Kuncewicz, P. Balakrishnan, M. B. Snuggs, and B. C. Kone Specific association of nitric oxide synthase-2 with Rac isoforms in activated murine macrophages Am J Physiol Renal Physiol, August 1, 2001; 281(2): F326 - F336. [Abstract] [Full Text] [PDF]

				J. W. Verlander, R. M. Moudy, W. G. Campbell, B. D. Cain, and C. S. Wingo Immunohistochemical localization of H-K-ATPase alpha 2c-subunit in rabbit kidney Am J Physiol Renal Physiol, August 1, 2001; 281(2): F357 - F365. [Abstract] [Full Text] [PDF]

				N. Laroche-Joubert, S. Marsy, and A. Doucet Cellular origin and hormonal regulation of K+-ATPase activities sensitive to Sch-28080 in rat collecting duct Am J Physiol Renal Physiol, December 1, 2000; 279(6): F1053 - F1059. [Abstract] [Full Text] [PDF]

				J. Codina, J. Cardwell, J. J. Gitomer, Y. Cui, B. C. Kone, and T. D. Dubose Jr. Sch-28080 depletes intracellular ATP selectively in mIMCD-3 cells Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1319 - C1326. [Abstract] [Full Text] [PDF]

				A. E. Frank, C. S. Wingo, and I. D. Weiner Effects of ammonia on bicarbonate transport in the cortical collecting duct Am J Physiol Renal Physiol, February 1, 2000; 278(2): F219 - F226. [Abstract] [Full Text] [PDF]

				J. Codina, T. A. Pressley, and T. D. DuBose Jr. The Colonic H+,K+-ATPase Functions as a Na+-dependent K+(NH4+)-ATPase in Apical Membranes from Rat Distal Colon J. Biol. Chem., July 9, 1999; 274(28): 19693 - 19698. [Abstract] [Full Text] [PDF]

				R. B. Silver and M. Soleimani H+-K+-ATPases: regulation and role in pathophysiological states Am J Physiol Renal Physiol, June 1, 1999; 276(6): F799 - F811. [Abstract] [Full Text] [PDF]

				F. Jaisser and A. T. Beggah The nongastric H+-K+-ATPases: molecular and functional properties Am J Physiol Renal Physiol, June 1, 1999; 276(6): F812 - F824. [Abstract] [Full Text] [PDF]

				S. J. Rulli, M. N. Horiba, E. Skripnikova, and E. C. Rabon Glu-857 Moderates K+-dependent Stimulation and SCH  28080-dependent Inhibition of the Gastric H,K-ATPase J. Biol. Chem., May 21, 1999; 274(21): 15245 - 15250. [Abstract] [Full Text] [PDF]

				I. D. Weiner, A. E. Frank, and C. S. Wingo Apical proton secretion by the inner stripe of the outer medullary collecting duct Am J Physiol Renal Physiol, April 1, 1999; 276(4): F606 - F613. [Abstract] [Full Text] [PDF]

				B. C. Kone and S. Higham Nitric oxide inhibits transcription of the Na+-K+-ATPase alpha 1-subunit gene in an MTAL cell line Am J Physiol Renal Physiol, April 1, 1999; 276(4): F614 - F621. [Abstract] [Full Text] [PDF]

				W. G. Campbell, I. D. Weiner, C. S. Wingo, and B. D. Cain H-K-ATPase in the RCCT-28A rabbit cortical collecting duct cell line Am J Physiol Renal Physiol, February 1, 1999; 276(2): F237 - F245. [Abstract] [Full Text] [PDF]

				A. V. Grishin and M. J. Caplan ATP1AL1, a Member of the Non-gastric H,K-ATPase Family, Functions as a Sodium Pump J. Biol. Chem., October 23, 1998; 273(43): 27772 - 27778. [Abstract] [Full Text] [PDF]

				S. M. Wall, P. Mehta, and T. D. DuBose Jr. Dietary K+ restriction upregulates total and Sch-28080-sensitive bicarbonate absorption in rat tIMCD Am J Physiol Renal Physiol, October 1, 1998; 275(4): F543 - F549. [Abstract] [Full Text] [PDF]

				J. Codina, J. T. Delmas-Mata, and T. D. DuBose Jr. Expression of HKalpha 2 protein is increased selectively in renal medulla by chronic hypokalemia Am J Physiol Renal Physiol, September 1, 1998; 275(3): F433 - F440. [Abstract] [Full Text] [PDF]

				N. B. Pestov, T. V. Korneenko, G. Adams, M. Tillekeratne, M. I. Shakhparonov, and N. N. Modyanov Nongastric H-K-ATPase in rodent prostate: lobe-specific expression and apical localization Am J Physiol Cell Physiol, April 1, 2002; 282(4): C907 - C916. [Abstract] [Full Text] [PDF]