HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Naito, M. Articles by Yamada, T. Search for Related Content PubMed PubMed Citation Articles by Naito, M. Articles by Yamada, T. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 5, 2905-2909, January 30, 1998

The Novel Fibronectin-binding Motif and Key Residues of Mycobacteria

From the Department of Oral Bacteriology, Nagasaki University School of Dentistry, 1-7-1 Sakamoto, Nagasaki 852, Japan

The binding motifs of the immunodominant antigen (Ag) -Ag (Ag 85 complex B) of Mycobacterium kansasii for human fibronectin were examined using digested fragments. We defined two fibronectin-binding epitopes on 27 amino acids from 84 to 110 and on 20 amino acids from 211 to 230. The epitopes were almost conserved in the closely related Ag 85 complex of other mycobacteria species. Inhibition of fibronectin binding to intact -Ag molecules was observed with peptide-(84-110), but not with peptide-(211-230). Peptide-(84-110) could also inhibit fibronectin binding to all components of the Ag 85 complex of Bacillus Calmette-Guérin (Ag 85A, Ag 85B, and Ag 85C). Further study with synthetic peptides defined 11 residues from 98 to 108 as the minimum motif. Six residues (⁹⁸FEWYYQ¹⁰³) were critical for interacting with fibronectin. The motif revealed no homology to other known prokaryotic and eukaryotic fibronectin-binding proteins. The defined motif of -Ag is novel and unique for mycobacteria.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				T. Katsube, S. Matsumoto, M. Takatsuka, M. Okuyama, Y. Ozeki, M. Naito, Y. Nishiuchi, N. Fujiwara, M. Yoshimura, T. Tsuboi, et al. Control of Cell Wall Assembly by a Histone-Like Protein in Mycobacteria J. Bacteriol., November 15, 2007; 189(22): 8241 - 8249. [Abstract] [Full Text] [PDF]

				L. Hall-Stoodley, G. Watts, J. E. Crowther, A. Balagopal, J. B. Torrelles, J. Robison-Cox, R. F. Bargatze, A. G. Harmsen, E. C. Crouch, and L. S. Schlesinger Mycobacterium tuberculosis Binding to Human Surfactant Proteins A and D, Fibronectin, and Small Airway Epithelial Cells under Shear Conditions. Infect. Immun., June 1, 2006; 74(6): 3587 - 3596. [Abstract] [Full Text] [PDF]

				S. Takamura, K. Matsuo, Y. Takebe, and Y. Yasutomi Ag85B of Mycobacteria Elicits Effective CTL Responses through Activation of Robust Th1 Immunity as a Novel Adjuvant in DNA Vaccine J. Immunol., August 15, 2005; 175(4): 2541 - 2547. [Abstract] [Full Text] [PDF]

				Y. Kumagai, H. Yagishita, A. Yajima, T. Okamoto, and K. Konishi Molecular Mechanism for Connective Tissue Destruction by Dipeptidyl Aminopeptidase IV Produced by the Periodontal Pathogen Porphyromonas gingivalis Infect. Immun., May 1, 2005; 73(5): 2655 - 2664. [Abstract] [Full Text] [PDF]

				K. Aoki, S. Matsumoto, Y. Hirayama, T. Wada, Y. Ozeki, M. Niki, P. Domenech, K. Umemori, S. Yamamoto, A. Mineda, et al. Extracellular Mycobacterial DNA-binding Protein 1 Participates in Mycobacterium-Lung Epithelial Cell Interaction through Hyaluronic Acid J. Biol. Chem., September 17, 2004; 279(38): 39798 - 39806. [Abstract] [Full Text] [PDF]

				D. R. Ronning, V. Vissa, G. S. Besra, J. T. Belisle, and J. C. Sacchettini Mycobacterium tuberculosis Antigen 85A and 85C Structures Confirm Binding Orientation and Conserved Substrate Specificity J. Biol. Chem., August 27, 2004; 279(35): 36771 - 36777. [Abstract] [Full Text] [PDF]

				W. S. Probert, J. H. Kim, M. Hook, and B. J. B. Johnson Mapping the Ligand-Binding Region of Borrelia burgdorferi Fibronectin-Binding Protein BBK32 Infect. Immun., June 1, 2001; 69(6): 4129 - 4133. [Abstract] [Full Text] [PDF]

				T. E. Secott, T. L. Lin, and C. C. Wu Fibronectin Attachment Protein Homologue Mediates Fibronectin Binding by Mycobacterium avium subsp. paratuberculosis Infect. Immun., April 1, 2001; 69(4): 2075 - 2082. [Abstract] [Full Text] [PDF]

				P. GILOT, Y. JOSSIN, and J. CONTENT Cloning, sequencing and characterisation of a Listeria monocytogenes gene encoding a fibronectin-binding protein J. Med. Microbiol., October 1, 2000; 49(10): 887 - 896. [Abstract] [Full Text] [PDF]

				C. Espitia, J. P. Laclette, M. Mondragon- Palomino, A. Amador, J. Campuzano, A. Martens, M. Singh, R. Cicero, Y. Zhang, and C. Moreno The PE-PGRS glycine-rich proteins of Mycobacterium tuberculosis : a new family of fibronectin-binding proteins? Microbiology, December 1, 1999; 145(12): 3487 - 3495. [Abstract] [Full Text] [PDF]

				W. Zhao, J. S. Schorey, R. Groger, P. M. Allen, E. J. Brown, and T. L. Ratliff Characterization of the Fibronectin Binding Motif for a Unique Mycobacterial Fibronectin Attachment Protein, FAP J. Biol. Chem., February 19, 1999; 274(8): 4521 - 4526. [Abstract] [Full Text] [PDF]