HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bertrand, F. Articles by Cherqui, G. Search for Related Content PubMed PubMed Citation Articles by Bertrand, F. Articles by Cherqui, G. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 5, 2931-2938, January 30, 1998

A Role for Nuclear Factor B in the Antiapoptotic Function of Insulin

France Bertrand, Azeddine Atfi, Axelle Cadoret, Gilles L'Allemain^¶, Hélène Robin, Olivier Lascols, Jacqueline Capeau, and Gisèle Cherqui

From INSERM U.402, Institut Fédératif de Recherche 65, Laboratoire de Biologie Cellulaire, Faculté de Médecine Saint-Antoine, 27 rue Chaligny, 75571 Paris, Cedex 12, France, the  INSERM U.482, Institut Fédératif de Recherche 65, Hôpital Saint-Antoine, 184 rue du Faubourg Saint-Antoine, 75571 Paris, Cedex 12 France, and the ^¶ Centre de Biochimie, CNRS, Unité Mixte de Recherche 134, Faculté des Sciences, Parc Valrose, 06108 Nice, Cedex 02, France

We previously reported that insulin activates nuclear factor B (NF-B) in Chinese hamster ovary (CHO)-R cells overexpressing wild-type insulin receptors (IRs) through a pathway requiring IR tyrosine kinase and Raf-1 kinase activities. We now investigated whether the activation of NF-B by insulin could serve an antiapoptotic function. Insulin (10⁹-10⁷ M) inhibited apoptosis induced by serum withdrawal in CHO-R cells in a concentration-dependent manner. Insulin antiapoptotic signaling: (i) was dependent on IR number and IR tyrosine kinase activity since it was reduced in parental CHO cells and was abolished in CHO-Y2 cells overexpressing IRs mutated at Tyr^1162/1163; (ii) was, like insulin activation of NF-B, dependent on Raf-1 but not on mitogen-activated protein kinase activity since both processes were decreased by the dominant-negative Raf-1 mutant Raf-C4 whereas they persisted in mitogen-activated protein kinase-depleted cells; and (iii) required NF-B activation since it was decreased by proteasome inhibitors and the dominant-negative IB- (A32/36) mutant and was mimicked by overexpression of the NF-B c-Rel subunit. We also show that insulin antiapoptotic signaling but not insulin activation of NF-B involved phosphatidylinositol 3-kinase (PI 3-kinase), as supported by the inhibition of the former but not of the latter process by the PI 3-kinase inhibitor LY294002. Inhibition of both NF-B and PI 3-kinase totally abolished insulin antiapoptotic signaling. Thus insulin exerts a specific antiapoptotic function which is dependent on IR tyrosine kinase activity and is mediated by both a Raf-1-dependent pathway that leads to NF-B activation and a PI 3-kinase-dependent pathway.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. Ren, Q. Guan, X. Zhong, B. Gong, Y. Sun, W. Xin, J. Guo, H. Wang, L. Gao, and J. Zhao Phosphatidylinositol 3-kinase/nuclear factor-{kappa}B signaling pathway is involved in the regulation of IGF-I on Fas-associated death domain-like interleukin-1-converting enzyme-inhibitory protein expression in cultured FRTL thyroid cells J. Mol. Endocrinol., June 1, 2007; 38(6): 619 - 625. [Abstract] [Full Text] [PDF]

				N. M. Al-Rasheed, G. B. Willars, and N. J. Brunskill C-Peptide Signals via G{alpha}i to Protect against TNF-{alpha}-Mediated Apoptosis of Opossum Kidney Proximal Tubular Cells J. Am. Soc. Nephrol., April 1, 2006; 17(4): 986 - 995. [Abstract] [Full Text] [PDF]

				T. Nakazawa, T. Chiba, E. Kaneko, K. Yui, M. Yoshida, and K. Shimokado Insulin Signaling in Arteries Prevents Smooth Muscle Apoptosis Arterioscler. Thromb. Vasc. Biol., April 1, 2005; 25(4): 760 - 765. [Abstract] [Full Text] [PDF]

				V. Navarro-Tableros, M. C. Sanchez-Soto, S. Garcia, and M. Hiriart Autocrine Regulation of Single Pancreatic {beta}-Cell Survival Diabetes, August 1, 2004; 53(8): 2018 - 2023. [Abstract] [Full Text] [PDF]

				J. F. Sanchez, L. F. Sniderhan, A. L. Williamson, S. Fan, S. Chakraborty-Sett, and S. B. Maggirwar Glycogen Synthase Kinase 3{beta}-Mediated Apoptosis of Primary Cortical Astrocytes Involves Inhibition of Nuclear Factor {kappa}B Signaling Mol. Cell. Biol., July 1, 2003; 23(13): 4649 - 4662. [Abstract] [Full Text] [PDF]

				T. J. Page, S. O'Brien, K. Holston, P. S. MacWilliams, C. R. Jefcoate, and C. J. Czuprynski 7,12-Dimethylbenz[a]anthracene-Induced Bone Marrow Toxicity Is p53-Dependent Toxicol. Sci., July 1, 2003; 74(1): 85 - 92. [Abstract] [Full Text] [PDF]

				J. S. Russell, U. Raju, G. J. Gumin, F. F. Lang, D. R. Wilson, T. Huet, and P. J. Tofilon Inhibition of Radiation-induced Nuclear Factor-{kappa}B Activation by an Anti-Ras Single-Chain Antibody Fragment: Lack of Involvement in Radiosensitization Cancer Res., April 1, 2002; 62(8): 2318 - 2326. [Abstract] [Full Text] [PDF]

				M. Muller, A. Morotti, and C. Ponzetto Activation of NF-{kappa}B Is Essential for Hepatocyte Growth Factor-Mediated Proliferation and Tubulogenesis Mol. Cell. Biol., February 15, 2002; 22(4): 1060 - 1072. [Abstract] [Full Text] [PDF]

				S. K. Pandey, H.-J. He, A. Chesley, M. Juhaszova, M. T. Crow, and M. Bernier Wortmannin-Sensitive Pathway Is Required for Insulin-Stimulated Phosphorylation of Inhibitor {kappa}B{alpha} Endocrinology, February 1, 2002; 143(2): 375 - 385. [Abstract] [Full Text] [PDF]

				S. Rousse, F. Lallemand, D. Montarras, C. Pinset, A. Mazars, C. Prunier, A. Atfi, and C. Dubois Transforming Growth Factor-beta Inhibition of Insulin-like Growth Factor-binding Protein-5 Synthesis in Skeletal Muscle Cells Involves a c-Jun N-terminal Kinase-dependent Pathway J. Biol. Chem., December 7, 2001; 276(50): 46961 - 46967. [Abstract] [Full Text] [PDF]

				J. Buteau, S. Foisy, C. J. Rhodes, L. Carpenter, T. J. Biden, and M. Prentki Protein Kinase C{zeta} Activation Mediates Glucagon-Like Peptide-1-Induced Pancreatic {beta}-Cell Proliferation Diabetes, October 1, 2001; 50(10): 2237 - 2243. [Abstract] [Full Text]

				S. Yang, H. Lin, and A. M. Diehl Fatty liver vulnerability to endotoxin-induced damage despite NF-{kappa}B induction and inhibited caspase 3 activation Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G382 - G392. [Abstract] [Full Text] [PDF]

				S. Yang, H. Z. Lin, J. Hwang, V. P. Chacko, and A. M. Diehl Hepatic Hyperplasia in Noncirrhotic Fatty Livers: Is Obesity-related Hepatic Steatosis a Premalignant Condition? Cancer Res., July 1, 2001; 61(13): 5016 - 5023. [Abstract] [Full Text] [PDF]

				C. Gründker, K. Schulz, A. R. Günthert, and G. Emons Luteinizing Hormone-Releasing Hormone Induces Nuclear Factor {kappa}B-Activation and Inhibits Apoptosis in Ovarian Cancer Cells J. Clin. Endocrinol. Metab., October 1, 2000; 85(10): 3815 - 3820. [Abstract] [Full Text]

				J. R. Wu-Wong, W. J. Chiou, and J. Wang Extracellular Signal-Regulated Kinases Are Involved in the Antiapoptotic Effect of Endothelin-1 J. Pharmacol. Exp. Ther., May 1, 2000; 293(2): 514 - 521. [Abstract] [Full Text]

				M. M. Remacle-Bonnet, F. L. Garrouste, S. Heller, F. André, J. L. Marvaldi, and G. J. Pommier Insulin-like Growth Factor-I Protects Colon Cancer Cells from Death Factor-induced Apoptosis by Potentiating Tumor Necrosis Factor {{alpha}}-induced Mitogen-activated Protein Kinase and Nuclear Factor {{kappa}}B Signaling Pathways Cancer Res., April 1, 2000; 60(7): 2007 - 2017. [Abstract] [Full Text]

				C. Desbois-Mouthon, A. Cadoret, M.-J. Blivet-Van Eggelpoel, F. Bertrand, M. Caron, A. Atfi, G. Cherqui, and J. Capeau Insulin-Mediated Cell Proliferation and Survival Involve Inhibition of c-Jun N-terminal Kinases through a Phosphatidylinositol 3-Kinase- and Mitogen-Activated Protein Kinase Phosphatase-1-Dependent Pathway Endocrinology, March 1, 2000; 141(3): 922 - 931. [Abstract] [Full Text] [PDF]

				C. Hermann, B. Assmus, C. Urbich, A. M. Zeiher, and S. Dimmeler Insulin-Mediated Stimulation of Protein Kinase Akt : A Potent Survival Signaling Cascade for Endothelial Cells Arterioscler. Thromb. Vasc. Biol., February 1, 2000; 20(2): 402 - 409. [Abstract] [Full Text] [PDF]

				F. Bertrand, C. Desbois-Mouthon, A. Cadoret, C. Prunier, H. Robin, J. Capeau, A. Atfi, and G. Cherqui Insulin Antiapoptotic Signaling Involves Insulin Activation of the Nuclear Factor kappa B-dependent Survival Genes Encoding Tumor Necrosis Factor Receptor-associated Factor 2 and Manganese-superoxide Dismutase J. Biol. Chem., October 22, 1999; 274(43): 30596 - 30602. [Abstract] [Full Text] [PDF]

				V. T. Heussler, J. Machado Jr, P. C. Fernandez, C. Botteron, C.-G. Chen, M. J. Pearse, and D. A. E. Dobbelaere The intracellular parasite Theileria parva protects infected T cells from apoptosis PNAS, June 22, 1999; 96(13): 7312 - 7317. [Abstract] [Full Text] [PDF]

				H. Marusawa, M. Hijikata, T. Chiba, and K. Shimotohno Hepatitis C Virus Core Protein Inhibits Fas- and Tumor Necrosis Factor Alpha-Mediated Apoptosis via NF-kappa B Activation J. Virol., June 1, 1999; 73(6): 4713 - 4720. [Abstract] [Full Text]

				G. A. Webster and N. D. Perkins Transcriptional Cross Talk between NF-kappa B and p53 Mol. Cell. Biol., May 1, 1999; 19(5): 3485 - 3495. [Abstract] [Full Text] [PDF]

				S. Heck, F. Lezoualc'h, S. Engert, and C. Behl Insulin-like Growth Factor-1-mediated Neuroprotection against Oxidative Stress Is Associated with Activation of Nuclear Factor kappa B J. Biol. Chem., April 2, 1999; 274(14): 9828 - 9835. [Abstract] [Full Text] [PDF]

				M. Bentires-Alj, A.-C. Hellin, M. Ameyar, S. Chouaib, M.-P. Merville, and V. Bours Stable Inhibition of Nuclear Factor {{kappa}}B in Cancer Cells Does Not Increase Sensitivity to Cytotoxic Drugs Cancer Res., February 1, 1999; 59(4): 811 - 815. [Abstract] [Full Text] [PDF]

				R. Wadgaonkar, K. M. Phelps, Z. Haque, A. J. Williams, E. S. Silverman, and T. Collins CREB-binding Protein Is a Nuclear Integrator of Nuclear Factor-kappa B and p53 Signaling J. Biol. Chem., January 22, 1999; 274(4): 1879 - 1882. [Abstract] [Full Text] [PDF]

				S. B. Maggirwar, P. D. Sarmiere, S. Dewhurst, and R. S. Freeman Nerve Growth Factor-Dependent Activation of NF-kappa B Contributes to Survival of Sympathetic Neurons J. Neurosci., December 15, 1998; 18(24): 10356 - 10365. [Abstract] [Full Text] [PDF]

				F. Besancon, A. Atfi, C. Gespach, Y. E. Cayre, and M.-F. Bourgeade Evidence for a role of NF-kappa B in the survival of hematopoietic cells mediated by interleukin 3 and the oncogenic TEL/platelet-derived growth factor receptor beta  fusion protein PNAS, July 7, 1998; 95(14): 8081 - 8086. [Abstract] [Full Text] [PDF]

				P. J. Antoine, F. Bertrand, M. Auclair, J. Magre, J. Capeau, and G. Cherqui Insulin Induction of Protein Kinase C{alpha} Expression Is Independent of Insulin Receptor Tyr1162/1163 Residues and Involves Mitogen-Activated Protein Kinase Kinase 1 and Sustained Activation of Nuclear p44MAPK Endocrinology, July 1, 1998; 139(7): 3133 - 3142. [Abstract] [Full Text] [PDF]

				D. Courilleau, E. Chastre, M. Sabbah, G. Redeuilh, A. Atfi, and J. Mester B-ind1, a Novel Mediator of Rac1 Signaling Cloned from Sodium Butyrate-treated Fibroblasts J. Biol. Chem., June 2, 2000; 275(23): 17344 - 17348. [Abstract] [Full Text] [PDF]

				M. Waltner-Law, M. C. Daniels, C. Sutherland, and D. K. Granner NF-kappa B Inhibits Glucocorticoid and cAMP-mediated Expression of the Phosphoenolpyruvate Carboxykinase Gene J. Biol. Chem., October 6, 2000; 275(41): 31847 - 31856. [Abstract] [Full Text] [PDF]

				F. Demarchi, R. Verardo, B. Varnum, C. Brancolini, and C. Schneider Gas6 Anti-apoptotic Signaling Requires NF-kappa B Activation J. Biol. Chem., August 17, 2001; 276(34): 31738 - 31744. [Abstract] [Full Text] [PDF]

				A. J. Barber, M. Nakamura, E. B. Wolpert, C. E. N. Reiter, G. M. Seigel, D. A. Antonetti, and T. W. Gardner Insulin Rescues Retinal Neurons from Apoptosis by a Phosphatidylinositol 3-Kinase/Akt-mediated Mechanism That Reduces the Activation of Caspase-3 J. Biol. Chem., August 24, 2001; 276(35): 32814 - 32821. [Abstract] [Full Text] [PDF]