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J Biol Chem, Vol. 273, Issue 5, 2939-2946, January 30, 1998
From the Biochemie Zentrum Heidelberg, Biosynthesis of plasma membrane sphingolipids
involves the coordinate action of enzymes localized to individual
compartments of the biosynthetic secretory pathway of proteins. These
stations include the endoplasmic reticulum and the Golgi apparatus.
Although a precise localization of all the enzymes that synthesize
glycosphingolipids has not been achieved to date, it is assumed that
the sequence of events in glycosphingolipid biosynthesis resembles that
in glycoprotein biosynthesis, i.e. that early reactions
occur in early stations (endoplasmic reticulum and cis/medial Golgi) of the pathway, and late reactions occur in late stations (trans Golgi/trans Golgi network).
Using truncated analogues of ceramide and glucosylceramide that allow
measurement of enzyme activities in intact membrane fractions, we have
reinvestigated the localization of individual enzymes involved in
glycosphingolipid biosynthesis and for the first time studied the
localization of lactosylceramide synthase after partial separation of
Golgi membranes as previously described (Trinchera, M., and Ghidoni, R. (1989) J. Biol. Chem. 264, 15766-15769). Here, we
show that the reactions involved in higher glycosphingolipid biosynthesis, including lactosylceramide synthesis, all reside in the
lumen of the late Golgi compartments from rat liver.
Functional Organization of the Golgi Apparatus in
Glycosphingolipid Biosynthesis
LACTOSYLCERAMIDE AND SUBSEQUENT GLYCOSPHINGOLIPIDS ARE FORMED IN
THE LUMEN OF THE LATE GOLGI
, Institut für Anatomie und Zellbiologie,
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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