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J Biol Chem, Vol. 273, Issue 5, 3051-3059, January 30, 1998
From the Department of Microbiology, Duke University Medical
Center, Durham, North Carolina 27710
Since DNA replication and transcription often
temporally and spatially overlap each other, the impact of one process
on the other is of considerable interest. We have reported previously that transcription is impeded at the replication termini of
Escherichia coli and Bacillus subtilis in a
polar mode and that, when transcription is allowed to invade a
replication terminus from the permissive direction, arrest of
replication fork at the terminus is abrogated. In the present report,
we have addressed four significant questions pertaining to the
mechanism of transcription impedance by the replication terminator
proteins. Is transcription arrested at the replication terminus or does
RNA polymerase dissociate from the DNA causing authentic transcription
termination? How does transcription cause abrogation of replication
fork arrest at the terminus? Are the points of arrest of the
replication fork and transcription the same or are these different? Are
eukaryotic RNA polymerases also arrested at prokaryotic replication
termini? Our results show that replication terminator proteins of
E. coli and B. subtilis arrest but do not terminate
transcription. Passage of an RNA transcript through the replication
terminus causes the dissociation of the terminator protein from the
terminus DNA, thus causing abrogation of replication fork arrest. DNA
and RNA chain elongation are arrested at different locations on the
terminator sites. Finally, although bacterial replication terminator
proteins blocked yeast RNA polymerases in a polar fashion, a yeast
transcription terminator protein (Reb1p) was unable to block T7 RNA
polymerase and E. coli DnaB helicase.
Mechanistic Studies on the Impact of Transcription on
Sequence-specific Termination of DNA Replication and Vice Versa
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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