![]()
|
|
||||||||
J Biol Chem, Vol. 273, Issue 5, 3082-3089, January 30, 1998
From the Biology Department, University of Massachusetts,
Amherst, Massachusetts 01003
To study the mechanism of heterochromatin
formation in vertebrate cells, we isolated nuclei from chicken
polymorphonuclear granulocytes and examined the chromatin organization.
We found granulocyte chromatin to remain insoluble after nuclease
digestion and to be resistant to swelling in low salt/high pH media.
Both insolubility and resistance to swelling were lost after washing with 0.3 M NaCl, a procedure that released two
abundant tissue-specific proteins from granulocyte nuclei. One of them
(42 kDa) is identified as MENT, a protein previously shown to be
associated with repressed chromatin from mature chicken erythrocytes.
We show that MENT is immunolocalized in granulocyte heterochromatin,
where it is one of the most abundant chromatin proteins (~2
molecules/200 base pairs of DNA). MENT is the first nuclear protein
structurally related to the serine protease inhibitor family. The other
abundant protein is similar to or identical with mim-1, a
myeloid-specific protein that is known to be stored in cell granules
and to associate with isolated nuclei. MENT (but not mim-1) binds
chromatin and free DNA, and, at its physiological protein/DNA ratio,
enhances compaction and the reversible
Mg2+-dependent self-association of nucleosome
arrays. MENT appears to promote the formation of heterochromatin by
acting as a "glue" within and between chains of nucleosomes.
Chromatin Structure in Granulocytes
A LINK BETWEEN TIGHT COMPACTION AND ACCUMULATION OF A
HETEROCHROMATIN-ASSOCIATED PROTEIN (MENT)
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
![]() |
P. C. Ong, S. McGowan, M. C. Pearce, J. A. Irving, W.-T. Kan, S. A. Grigoryev, B. Turk, G. A. Silverman, K. Brix, S. P. Bottomley, et al. DNA Accelerates the Inhibition of Human Cathepsin V by Serpins J. Biol. Chem., December 21, 2007; 282(51): 36980 - 36986. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. P. Kransdorf, S. Z. Wang, S. Z. Zhu, T. B. Langston, J. W. Rupon, and G. D. Ginder MBD2 is a critical component of a methyl cytosine-binding protein complex isolated from primary erythroid cells Blood, October 15, 2006; 108(8): 2836 - 2845. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. A. Bulynko, L. C. Hsing, R. W. Mason, D. J. Tremethick, and S. A. Grigoryev Cathepsin L stabilizes the histone modification landscape on the y chromosome and pericentromeric heterochromatin. Mol. Cell. Biol., June 1, 2006; 26(11): 4172 - 4184. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. Lukasova, Z. Koristek, M. Falk, S. Kozubek, S. Grigoryev, M. Kozubek, V. Ondrej, and I. Kroupova Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities J. Leukoc. Biol., January 1, 2005; 77(1): 100 - 111. [Abstract] [Full Text] [PDF] |
||||
![]() |
V. L. Nelson-DeGrave, J. K. Wickenheisser, J. E. Cockrell, J. R. Wood, R. S. Legro, J. F. Strauss III, and J. M. McAllister Valproate Potentiates Androgen Biosynthesis in Human Ovarian Theca Cells Endocrinology, February 1, 2004; 145(2): 799 - 808. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. M. Springhetti, N. E. Istomina, J. C. Whisstock, T. Nikitina, C. L. Woodcock, and S. A. Grigoryev Role of the M-loop and Reactive Center Loop Domains in the Folding and Bridging of Nucleosome Arrays by MENT J. Biol. Chem., October 31, 2003; 278(44): 43384 - 43393. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. E. Istomina, S. S. Shushanov, E. M. Springhetti, V. L. Karpov, I. A. Krasheninnikov, K. Stevens, K. S. Zaret, P. B. Singh, and S. A. Grigoryev Insulation of the Chicken {beta}-Globin Chromosomal Domain from a Chromatin-Condensing Protein, MENT Mol. Cell. Biol., September 15, 2003; 23(18): 6455 - 6468. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. H. Fischer, P. Taysavang, and S. M. Jhiang Nuclear Envelope Irregularity Is Induced by RET/PTC During Interphase Am. J. Pathol., September 1, 2003; 163(3): 1091 - 1100. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Pivot-Pajot, C. Caron, J. Govin, A. Vion, S. Rousseaux, and S. Khochbin Acetylation-Dependent Chromatin Reorganization by BRDT, a Testis-Specific Bromodomain-Containing Protein Mol. Cell. Biol., August 1, 2003; 23(15): 5354 - 5365. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Nakashima, T. Hagiwara, and M. Yamada Nuclear Localization of Peptidylarginine Deiminase V and Histone Deimination in Granulocytes J. Biol. Chem., December 13, 2002; 277(51): 49562 - 49568. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. A. Irving, S. S. Shushanov, R. N. Pike, E. Y. Popova, D. Bromme, T. H. T. Coetzer, S. P. Bottomley, I. A. Boulynko, S. A. Grigoryev, and J. C. Whisstock Inhibitory Activity of a Heterochromatin-associated Serpin (MENT) against Papain-like Cysteine Proteinases Affects Chromatin Structure and Blocks Cell Proliferation J. Biol. Chem., April 5, 2002; 277(15): 13192 - 13201. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. T. Georgel, M. A. Palacios DeBeer, G. Pietz, C. A. Fox, and J. C. Hansen Sir3-dependent assembly of supramolecular chromatin structures in vitro PNAS, July 5, 2001; (2001) 151258798. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Misteli Protein Dynamics: Implications for Nuclear Architecture and Gene Expression Science, February 2, 2001; 291(5505): 843 - 847. [Abstract] [Full Text] [PDF] |
||||
![]() |
Z. H. Lu, H. Xu, and G. H. Leno DNA Replication in Quiescent Cell Nuclei: Regulation by the Nuclear Envelope and Chromatin Structure Mol. Biol. Cell, December 1, 1999; 10(12): 4091 - 4106. [Abstract] [Full Text] |
||||
![]() |
S. A. Grigoryev, J. Bednar, and C. L. Woodcock MENT, a Heterochromatin Protein That Mediates Higher Order Chromatin Folding, Is a New Serpin Family Member J. Biol. Chem., February 26, 1999; 274(9): 5626 - 5636. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. T. Georgel, M. A. Palacios DeBeer, G. Pietz, C. A. Fox, and J. C. Hansen Sir3-dependent assembly of supramolecular chromatin structures in vitro PNAS, July 17, 2001; 98(15): 8584 - 8589. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. A. Irving, R. N. Pike, A. M. Lesk, and J. C. Whisstock Phylogeny of the Serpin Superfamily: Implications of Patterns of Amino Acid Conservation for Structure and Function Genome Res., December 1, 2000; 10(12): 1845 - 1864. [Abstract] [Full Text] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |