JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Novina, C. D.
Right arrow Articles by Roy, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Novina, C. D.
Right arrow Articles by Roy, A. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 50, 33443-33448, December 11, 1998

Regulation of TFII-I Activity by Phosphorylation

Carl D. Novina, Venugopalan Cheriyath, and Ananda L. Roy

From the Department of Pathology and the Program in Immunology, Sackler School of Graduate Studies, Tufts University School of Medicine, Boston, Massachusetts 02111

The transcription factor TFII-I binds to distinct promoter sequences including an initiator element in several eukaryotic genes. Here we demonstrate that TFII-I is phosphorylated in vivo at serine/threonine and tyrosine residues in the absence of any apparent extracellular signals. This "basal" phosphorylation of TFII-I is not required and does not affect its specific DNA binding, but is critical for its in vitro transcriptional properties via the Vbeta promoter. To better assess the functional role of phosphorylation in regulating TFII-I activity, we focused on tyrosine phosphorylation of TFII-I. Ectopically expressed recombinant TFII-I, like its native counterpart, exhibits tyrosine phosphorylation in the absence of distinct extracellular signals. More important, mutation of a potential consensus tyrosine phosphorylation site in TFII-I leads to severe reduction in its basal transcriptional activation of the Vbeta promoter in vivo. Taken together, these data suggest that tyrosine phosphorylation of TFII-I is important for its initiator-dependent transcriptional activity.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
Z. P. Desgranges, J. Ahn, M. B. Lazebnik, T. Ashworth, C. Lee, R. C. Pestell, N. Rosenberg, C. Prives, and A. L. Roy
Inhibition of TFII-I-Dependent Cell Cycle Regulation by p53
Mol. Cell. Biol., December 15, 2005; 25(24): 10940 - 10952.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Stasyk, A. Dubrovska, M. Lomnytska, I. Yakymovych, C. Wernstedt, C.-H. Heldin, U. Hellman, and S. Souchelnytskyi
Phosphoproteome Profiling of Transforming Growth Factor (TGF)-{beta} Signaling: Abrogation of TGF{beta}1-dependent Phosphorylation of Transcription Factor-II-I (TFII-I) Enhances Cooperation of TFII-I and Smad3 in Transcription
Mol. Biol. Cell, October 1, 2005; 16(10): 4765 - 4780.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
H. Youn, Y. Koo, I. Ji, and T. H. Ji
An Upstream Initiator-Like Element Suppresses Transcription of the Rat Luteinizing Hormone Receptor Gene
Mol. Endocrinol., May 1, 2005; 19(5): 1318 - 1328.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hong, M.-y. Lin, J.-m. Huang, P. Baumeister, S. Hakre, A. L. Roy, and A. S. Lee
Transcriptional Regulation of the Grp78 Promoter by Endoplasmic Reticulum Stress: ROLE OF TFII-I AND ITS TYROSINE PHOSPHORYLATION
J. Biol. Chem., April 29, 2005; 280(17): 16821 - 16828.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Rajaiya, M. Hatfield, J. C. Nixon, D. J. Rawlings, and C. F. Webb
Bruton's Tyrosine Kinase Regulates Immunoglobulin Promoter Activation in Association with the Transcription Factor Bright
Mol. Cell. Biol., March 15, 2005; 25(6): 2073 - 2084.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
E. S. Hwang, S. J. Szabo, P. L. Schwartzberg, and L. H. Glimcher
T Helper Cell Fate Specified by Kinase-Mediated Interaction of T-bet with GATA-3
Science, January 21, 2005; 307(5708): 430 - 433.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Sacristan, M. I. Tussie-Luna, S. M. Logan, and A. L. Roy
Mechanism of Bruton's Tyrosine Kinase-mediated Recruitment and Regulation of TFII-I
J. Biol. Chem., February 20, 2004; 279(8): 7147 - 7158.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. E. Casteel, S. Zhuang, T. Gudi, J. Tang, M. Vuica, S. Desiderio, and R. B. Pilz
cGMP-dependent Protein Kinase Ibeta Physically and Functionally Interacts with the Transcriptional Regulator TFII-I
J. Biol. Chem., August 23, 2002; 277(35): 32003 - 32014.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Cheriyath, Z. P. Desgranges, and A. L. Roy
c-Src-dependent Transcriptional Activation of TFII-I
J. Biol. Chem., June 14, 2002; 277(25): 22798 - 22805.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D.-W. Kim and B. H. Cochran
JAK2 Activates TFII-I and Regulates Its Interaction with Extracellular Signal-Regulated Kinase
Mol. Cell. Biol., May 15, 2001; 21(10): 3387 - 3397.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
R. Parker, T. Phan, P. Baumeister, B. Roy, V. Cheriyath, A. L. Roy, and A. S. Lee
Identification of TFII-I as the Endoplasmic Reticulum Stress Response Element Binding Factor ERSF: Its Autoregulation by Stress and Interaction with ATF6
Mol. Cell. Biol., May 1, 2001; 21(9): 3220 - 3233.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
C. F. Webb, Y. Yamashita, N. Ayers, S. Evetts, Y. Paulin, M. E. Conley, and E. A. Smith
The Transcription Factor Bright Associates with Bruton's Tyrosine Kinase, the Defective Protein in Immunodeficiency Disease
J. Immunol., December 15, 2000; 165(12): 6956 - 6965.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. M. Mobley and L. Sealy
The Rous Sarcoma Virus Long Terminal Repeat Promoter Is Regulated by TFII-I
J. Virol., July 15, 2000; 74(14): 6511 - 6519.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
C. D. Novina, S. Kumar, U. Bajpai, V. Cheriyath, K. Zhang, S. Pillai, H. H. Wortis, and A. L. Roy
Regulation of Nuclear Localization and Transcriptional Activity of TFII-I by Bruton's Tyrosine Kinase
Mol. Cell. Biol., July 1, 1999; 19(7): 5014 - 5024.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Cheriyath and A. L. Roy
Alternatively Spliced Isoforms of TFII-I. COMPLEX FORMATION, NUCLEAR TRANSLOCATION, AND DIFFERENTIAL GENE REGULATION
J. Biol. Chem., August 18, 2000; 275(34): 26300 - 26308.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Cheriyath and A. L. Roy
Structure-Function Analysis of TFII-I. ROLES OF THE N-TERMINAL END, BASIC REGION, AND I-REPEATS
J. Biol. Chem., March 9, 2001; 276(11): 8377 - 8383.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Egloff and S. Desiderio
Identification of Phosphorylation Sites for Bruton's Tyrosine Kinase within the Transcriptional Regulator BAP/TFII-I
J. Biol. Chem., July 20, 2001; 276(30): 27806 - 27815.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.