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J Biol Chem, Vol. 273, Issue 50, 33795-33802, December 11, 1998
From the Department of Biological Chemistry, University of
California, Irvine, California 92697-1700
Five purified protein components, RNA polymerase
I, Rrn3p, core factor, TBP (TATA-binding protein), and upstream
activation factor, are sufficient for high level transcription in
vitro from the Saccharomyces cerevisiae rDNA
promoter. Rrn3p and pol I form a complex in solution that is active in
specific initiation. Three protein components, pol I, Rrn3p, and core
factor, and promoter sequence to 
38, suffice for basal transcription.
Unlike pol II and pol III, yeast pol I basal transcription does not
require TBP. Instead, TBP, upstream activation factor, and the upstream element of the promoter together stimulate pol I basal transcription to
a fully activated level. The role of TBP in pol I transcription is
fundamentally different from its role in pol II or pol III transcription.
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