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J Biol Chem, Vol. 273, Issue 51, 33885-33888, December 18, 1998

COMMUNICATION
Cloning and Characterization of the 5'-Flanking Region of the Human Growth Hormone Secretagogue Receptor Gene

From the Third Division, Department of Medicine, Kobe University School of Medicine, Kobe 650-0017, Japan

Recently, the growth hormone secretagogue receptor (GHS-R) cDNA has been isolated from the pituitary and hypothalamus. To evaluate the regulation of human (h) GHS-R gene expression, we cloned the hGHS-R gene containing the 5'-flanking region of 0.6-2.9 kilobase pairs. Analysis of the hGHS-R transcripts with 5'-rapid amplification of cDNA ends suggested that the putative transcription initiation site was approximately 453 base pairs upstream of the translation initiation site (+1). There is no typical TATA, CAAT, or GC box but an initiator-like sequence and putative binding sites for several transcription factors around the putative transcription start site. The 5'-flanking region inserted into a luciferase reporter vector had promoter activity in GH₃ cells but had activity indistinguishable from background in HeLa or EP1 cells. The hGHS-R promoter activity in GH₃ cells increased by deletion of nucleotides from 1224 to 734, whereas it was decreased by further deletion from 734 to 608. Knowledge of the promoter region of the hGHS-R gene will facilitate elucidation of its transcriptional control.

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