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J Biol Chem, Vol. 273, Issue 51, 33885-33888, December 18, 1998
From the Third Division, Department of Medicine, Kobe University
School of Medicine, Kobe 650-0017, Japan
Recently, the growth hormone secretagogue
receptor (GHS-R) cDNA has been isolated from the pituitary and
hypothalamus. To evaluate the regulation of human (h) GHS-R gene
expression, we cloned the hGHS-R gene containing the 5'-flanking region
of 0.6-2.9 kilobase pairs. Analysis of the hGHS-R transcripts with
5'-rapid amplification of cDNA ends suggested that the putative
transcription initiation site was approximately 
453 base pairs
upstream of the translation initiation site (+1). There is no typical
TATA, CAAT, or GC box but an initiator-like sequence and putative
binding sites for several transcription factors around the putative
transcription start site. The 5'-flanking region inserted into a
luciferase reporter vector had promoter activity in
GH3 cells but had activity indistinguishable from
background in HeLa or EP1 cells. The hGHS-R promoter activity in
GH3 cells increased by deletion of nucleotides from 1224
to 734, whereas it was decreased by further deletion from 734 to
608. Knowledge of the promoter region of the hGHS-R gene will
facilitate elucidation of its transcriptional control.
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