HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zeilinger, S. Articles by Kubicek, C. P. Search for Related Content PubMed PubMed Citation Articles by Zeilinger, S. Articles by Kubicek, C. P. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 51, 34463-34471, December 18, 1998

Two Adjacent Protein Binding Motifs in the cbh2 (Cellobiohydrolase II-encoding) Promoter of the Fungus Hypocrea jecorina (Trichoderma reesei) Cooperate in the Induction by Cellulose

From the Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, Technische Universität Wien, Getreidemarkt 9/1725, A-1060 Wien, Austria

The cellulase system of the filamentous fungus Hypocrea jecorina (Trichoderma reesei) consists of several cellobiohydrolases, endoglucanases, and -glucosidases, encoded by separate genes, which are coordinately expressed in the presence of cellulose or the disaccharide sophorose. Using cell-free extracts from sophorose-induced and noninduced mycelia and various fragments of the cbh2 promoter of H. jecorina in electrophoretic mobility shift assay (EMSA) analysis and performing in vitro and in vivo footprinting analysis, we detected the nucleotide sequence 5'-ATTGGGTAATA-3' (consequently named cbh2-activating element (CAE)) to bind a protein complex with different migration in EMSA of induced and noninduced cell-free extracts. EMSA analysis, employing oligonucleotide fragments containing specifically mutated versions of CAE, revealed that protein binding requires the presence of an intact copy of either one of two adjacent motifs: a CCAAT (=ATTGG) box on the template strand and a GTAATA box on the coding strand, whereas a simultaneous mutation in both completely abolished binding. H. jecorina transformants, containing correspondingly mutated versions of the cbh2 promoter fused to the Escherichia coli hph gene as a reporter, expressed hph in a manner paralleling the efficacy of CAE-protein complex formation in EMSA, suggesting that the presence of either of both motifs is required for induction of cbh2 gene transcription. Antibody supershift experiments with anti-HapC antiserum as well as EMSA competition experiments with CCAAT binding promoter fragments of the Aspergillus nidulans amdS promoter suggest that the H. jecorina CCAAT box binding complex contains a homologue of HapC. The nature of the adjacent, GTAATA-binding protein(s) and its cooperation with the HapC homologue in cbh2 gene induction is discussed.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. Paloheimo, A. Mantyla, J. Kallio, T. Puranen, and P. Suominen Increased Production of Xylanase by Expression of a Truncated Version of the xyn11A Gene from Nonomuraea flexuosa in Trichoderma reesei Appl. Envir. Microbiol., May 15, 2007; 73(10): 3215 - 3224. [Abstract] [Full Text] [PDF]

				R. Rauscher, E. Wurleitner, C. Wacenovsky, N. Aro, A. R. Stricker, S. Zeilinger, C. P. Kubicek, M. Penttila, and R. L. Mach Transcriptional Regulation of xyn1, Encoding Xylanase I, in Hypocrea jecorina Eukaryot. Cell, March 1, 2006; 5(3): 447 - 456. [Abstract] [Full Text] [PDF]

				M. Paloheimo, A. Mantyla, J. Kallio, and P. Suominen High-Yield Production of a Bacterial Xylanase in the Filamentous Fungus Trichoderma reesei Requires a Carrier Polypeptide with an Intact Domain Structure Appl. Envir. Microbiol., December 1, 2003; 69(12): 7073 - 7082. [Abstract] [Full Text] [PDF]

				E. Wurleitner, L. Pera, C. Wacenovsky, A. Cziferszky, S. Zeilinger, C. P. Kubicek, and R. L. Mach Transcriptional Regulation of xyn2 in Hypocrea jecorina Eukaryot. Cell, February 1, 2003; 2(1): 150 - 158. [Abstract] [Full Text] [PDF]

				N. Aro, M. Ilmen, A. Saloheimo, and M. Penttila ACEI of Trichoderma reesei Is a Repressor of Cellulase and Xylanase Expression Appl. Envir. Microbiol., January 1, 2003; 69(1): 56 - 65. [Abstract] [Full Text] [PDF]

				R. P. de Vries and J. Visser Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides Microbiol. Mol. Biol. Rev., December 1, 2001; 65(4): 497 - 522. [Abstract] [Full Text] [PDF]

				A. Saloheimo, N. Aro, M. Ilmen, and M. Penttila Isolation of the ace1 Gene Encoding a Cys2-His2 Transcription Factor Involved in Regulation of Activity of the Cellulase Promoter cbh1 of Trichoderma reesei J. Biol. Chem., February 25, 2000; 275(8): 5817 - 5825. [Abstract] [Full Text] [PDF]

				M. M. C. Gielkens, E. Dekkers, J. Visser, and L. H. de Graaff Two Cellobiohydrolase-Encoding Genes from Aspergillus niger Require D-Xylose and the Xylanolytic Transcriptional Activator XlnR for Their Expression Appl. Envir. Microbiol., October 1, 1999; 65(10): 4340 - 4345. [Abstract] [Full Text]