| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 52, 34933-34940, December 25, 1998
§,,,
,,,,, and
From the Nerve growth factor (NGF) is a neurotrophin that
induces neuritogenic and trophic signals by binding to TrkA and/or p75
receptors. We report a comparative study of the binding,
internalization, and biological activity of NGF versus that
of NGF in association with an anti-NGF monoclonal antibody (mAb NGF30),
directed against the C termini of NGF. NGF·mAb complexes do not bind
p75 effectively but bind TrkA with high affinity. After binding,
NGF·mAb complexes stimulate internalization faster and to a larger
degree than NGF. NGF·mAb-induced activation of TrkA, Shc, and MAPK is
transient compared with NGF-induced activation; yet NGF and NGF·mAb
afford identical trophic responses. In contrast, NGF induces
Suc-1-associated neurotrophic activating protein phosphorylation and
neuritogenic differentiation, but NGF·mAb does not. Thus, an absolute
separation of trophic and neuritogenic function is seen for NGF·mAb,
suggesting that biological response modifiers of neurotrophins can
afford ligands with selected activities.
Department of Pharmacology and Therapeutics,
§ Oncology/Cancer Center, and Department of Cell
Biology, Department of Biological Chemistry,
Finch University of Health Sciences/The Chicago Medical School,
North Chicago, Illinois 60064
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
This article has been cited by other articles:
|
|
 |
|
  L. Ivanisevic, W. Zheng, S. B. Woo, K. E. Neet, and H. U. Saragovi TrkA Receptor "Hot Spots" for Binding of NT-3 as a Heterologous Ligand J. Biol. Chem., June 8, 2007; 282(23): 16754 - 16763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Valdez, W. Akmentin, P. Philippidou, R. Kuruvilla, D. D. Ginty, and S. Halegoua Pincher-Mediated Macroendocytosis Underlies Retrograde Signaling by Neurotrophin Receptors J. Neurosci., May 25, 2005; 25(21): 5236 - 5247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Bruno, P. B. S. Clarke, A. Seltzer, R. Quirion, K. Burgess, A. C. Cuello, and H. U. Saragovi Long-Lasting Rescue of Age-Associated Deficits in Cognition and the CNS Cholinergic Phenotype by a Partial Agonist Peptidomimetic Ligand of TrkA J. Neurosci., September 15, 2004; 24(37): 8009 - 8018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Lad, D. A. Peterson, R. A. Bradshaw, and K. E. Neet Individual and Combined Effects of TrkA and p75NTR Nerve Growth Factor Receptors: A ROLE FOR THE HIGH AFFINITY RECEPTOR SITE J. Biol. Chem., June 27, 2003; 278(27): 24808 - 24817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jullien, V. Guili, L. F. Reichardt, and B. B. Rudkin Molecular Kinetics of Nerve Growth Factor Receptor Trafficking and Activation J. Biol. Chem., October 4, 2002; 277(41): 38700 - 38708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yan, Y. Liang, K. D. Nylander, and N. F. Schor TrkA as a Life and Death Receptor: Receptor Dose as a Mediator of Function Cancer Res., September 1, 2002; 62(17): 4867 - 4875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shao, W. Akmentin, J. J. Toledo-Aral, J. Rosenbaum, G. Valdez, J. B. Cabot, B. S. Hilbush, and S. Halegoua Pincher, a pinocytic chaperone for nerve growth factor/TrkA signaling endosomes J. Cell Biol., May 13, 2002; 157(4): 679 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yan, Y. Liang, K. D. Nylander, J. Wong, R. M. Rudavsky, H. U. Saragovi, and N. F. Schor p75-Nerve Growth Factor as an Antiapoptotic Complex: Independence versus Cooperativity in Protection from Enediyne Chemotherapeutic Agents Mol. Pharmacol., April 1, 2002; 61(4): 710 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Butowt and C. S. von Bartheld Sorting of Internalized Neurotrophins into an Endocytic Transcytosis Pathway via the Golgi System: Ultrastructural Analysis in Retinal Ganglion Cells J. Neurosci., November 15, 2001; 21(22): 8915 - 8930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. York, D. C. Molliver, S. S. Grewal, P. E. Stenberg, E. W. McCleskey, and P. J. S. Stork Role of Phosphoinositide 3-Kinase and Endocytosis in Nerve Growth Factor-Induced Extracellular Signal-Regulated Kinase Activation via Ras and Rap1 Mol. Cell. Biol., November 1, 2000; 20(21): 8069 - 8083. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-z. Zhang, D. B. Moheban, B. R. Conway, A. Bhattacharyya, and R. A. Segal Cell Surface Trk Receptors Mediate NGF-Induced Survival While Internalized Receptors Regulate NGF-Induced Differentiation J. Neurosci., August 1, 2000; 20(15): 5671 - 5678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Maliartchouk, T. Debeir, N. Beglova, A. C. Cuello, K. Gehring, and H. U. Saragovi Genuine Monovalent Ligands of TrkA Nerve Growth Factor Receptors Reveal a Novel Pharmacological Mechanism of Action J. Biol. Chem., March 31, 2000; 275(14): 9946 - 9956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L. Watson, H. M. Heerssen, D. B. Moheban, M. Z. Lin, C. M. Sauvageot, A. Bhattacharyya, S. L. Pomeroy, and R. A. Segal Rapid Nuclear Responses to Target-Derived Neurotrophins Require Retrograde Transport of Ligand-Receptor Complex J. Neurosci., September 15, 1999; 19(18): 7889 - 7900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. F. Csar, N. J. Wilson, K.-A. McMahon, D. C. Marks, T. L. Beecroft, A. C. Ward, G. A. Whitty, V. Kanangasundarum, and J. A. Hamilton Proteomic Analysis of Macrophage Differentiation. p46/52Shc TYROSINE PHOSPHORYLATION IS REQUIRED FOR CSF-1-MEDIATED MACROPHAGE DIFFERENTIATION J. Biol. Chem., July 6, 2001; 276(28): 26211 - 26217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Zaccaro, L. Ivanisevic, P. Perez, S. O. Meakin, and H. U. Saragovi p75 Co-receptors Regulate Ligand-dependent and Ligand-independent Trk Receptor Activation, in Part by Altering Trk Docking Subdomains J. Biol. Chem., August 10, 2001; 276(33): 31023 - 31029. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
