HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sleeman, M. W. Articles by Czech, M. P. Search for Related Content PubMed PubMed Citation Articles by Sleeman, M. W. Articles by Czech, M. P.

J Biol Chem, Vol. 273, Issue 6, 3132-3135, February 6, 1998

COMMUNICATION
Association of Acyl-CoA Synthetase-1 with GLUT4-containing Vesicles

Mark W. Sleeman, Niles P. Donegan, Robin Heller-Harrison, William S. Lane, and Michael P. Czech

From the Program in Molecular Medicine and the Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester, Massachusetts 01605 and the  Harvard Microchemistry Facility, Harvard University, Cambridge, Massachusetts 02318

GLUT4, the glucose transporter present in insulin-sensitive tissues, resides in intracellular vesicular structures and translocates to the cell surface in response to insulin. In an attempt to identify proteins present in these structures, GLUT4-enriched vesicles prepared from rat adipocytes treated with or without insulin were prepared by sucrose velocity gradient centrifugation and immunoadsorbed with anti-GLUT4 antibody. We report here the sequence identification by high performance liquid chromatography-ion trap mass spectrometry of a p75 protein band, long chain acyl-CoA synthetase-1, specifically present in immunoadsorbed GLUT4-containing vesicles but not in vesicles adsorbed by nonimmune serum. Acyl-CoA synthetase activity detected in GLUT4-enriched vesicles prepared by gradient centrifugation from insulin-treated adipocytes was decreased to about the same extent as GLUT4 protein. Additionally, immunoadsorbed GLUT4 vesicles were found to catalyze palmitoylation of proteins when incubated with labeled palmitate, a pathway that requires palmitate esterification with CoA. These data indicate that the insulin-sensitive membrane compartment that sequesters GLUT4 in fat cells contains long chain acyl-CoA synthetase-1 and its product fatty acyl-CoA, shown previously to be required for budding and fusion in membrane trafficking processes.

---

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.

This article has been cited by other articles:

				E. Soupene and F. A. Kuypers Mammalian Long-Chain Acyl-CoA Synthetases Experimental Biology and Medicine, May 1, 2008; 233(5): 507 - 521. [Abstract] [Full Text] [PDF]

				H. A. Parkes, E. Preston, D. Wilks, M. Ballesteros, L. Carpenter, L. Wood, E. W. Kraegen, S. M. Furler, and G. J. Cooney Overexpression of acyl-CoA synthetase-1 increases lipid deposition in hepatic (HepG2) cells and rodent liver in vivo Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E737 - E744. [Abstract] [Full Text] [PDF]

				B. Kiens Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance Physiol Rev, January 1, 2006; 86(1): 205 - 243. [Abstract] [Full Text] [PDF]

				J. D. Weimar, C. C. DiRusso, R. Delio, and P. N. Black Functional Role of Fatty Acyl-Coenzyme A Synthetase in the Transmembrane Movement and Activation of Exogenous Long-chain Fatty Acids. AMINO ACID RESIDUES WITHIN THE ATP/AMP SIGNATURE MOTIF OF ESCHERICHIA COLI FadD ARE REQUIRED FOR ENZYME ACTIVITY AND FATTY ACID TRANSPORT J. Biol. Chem., August 9, 2002; 277(33): 29369 - 29376. [Abstract] [Full Text] [PDF]

				V. A. ZAMMIT Insulin Stimulation of Hepatic Triacylglycerol Secretion in the Insulin-Replete State: Implications for the Etiology of Peripheral Insulin Resistance Ann. N.Y. Acad. Sci., June 1, 2002; 967(1): 52 - 65. [Abstract] [Full Text] [PDF]

				G. J. COONEY, A. L. THOMPSON, S. M. FURLER, J. YE, and E. W. KRAEGEN Muscle Long-Chain Acyl CoA Esters and Insulin Resistance Ann. N.Y. Acad. Sci., June 1, 2002; 967(1): 196 - 207. [Abstract] [Full Text] [PDF]

				H. Katagiri, T. Asano, T. Yamada, T. Aoyama, Y. Fukushima, M. Kikuchi, T. Kodama, and Y. Oka Acyl-Coenzyme A Dehydrogenases Are Localized on GLUT4-Containing Vesicles via Association with Insulin-Regulated Aminopeptidase in a Manner Dependent on Its Dileucine Motif Mol. Endocrinol., May 1, 2002; 16(5): 1049 - 1059. [Abstract] [Full Text] [PDF]

				G. F. Lewis, A. Carpentier, K. Adeli, and A. Giacca Disordered Fat Storage and Mobilization in the Pathogenesis of Insulin Resistance and Type 2 Diabetes Endocr. Rev., April 1, 2002; 23(2): 201 - 229. [Abstract] [Full Text] [PDF]

				S. Kristiansen, J. N. Nielsen, S. Bourgoin, A. Klip, M. Franco, and E. A. Richter GLUT-4 translocation in skeletal muscle studied with a cell-free assay: involvement of phospholipase D Am J Physiol Endocrinol Metab, September 1, 2001; 281(3): E608 - E618. [Abstract] [Full Text] [PDF]

				A. L. Thompson, M. Y.-C. Lim-Fraser, E. W. Kraegen, and G. J. Cooney Effects of individual fatty acids on glucose uptake and glycogen synthesis in soleus muscle in vitro Am J Physiol Endocrinol Metab, September 1, 2000; 279(3): E577 - E584. [Abstract] [Full Text] [PDF]

				W. Lee, J. Ryu, J. Hah, T. Tsujita, and C. Y. Jung Association of Carboxyl Esterase with Facilitative Glucose Transporter Isoform 4 (GLUT4) Intracellular Compartments in Rat Adipocytes and Its Possible Role in Insulin-induced GLUT4 Recruitment J. Biol. Chem., March 31, 2000; 275(14): 10041 - 10046. [Abstract] [Full Text] [PDF]

				M. Hashiramoto and D. E. James Characterization of Insulin-Responsive GLUT4 Storage Vesicles Isolated from 3T3-L1 Adipocytes Mol. Cell. Biol., January 1, 2000; 20(1): 416 - 427. [Abstract] [Full Text]

				W. Lee, J. Ryu, R. P. Souto, P. F. Pilch, and C. Y. Jung Separation and Partial Characterization of Three Distinct Intracellular GLUT4 Compartments in Rat Adipocytes. SUBCELLULAR FRACTIONATION WITHOUT HOMOGENIZATION J. Biol. Chem., December 31, 1999; 274(53): 37755 - 37762. [Abstract] [Full Text] [PDF]

				S. Basu-Modak, O. Braissant, P. Escher, B. Desvergne, P. Honegger, and W. Wahli Peroxisome Proliferator-activated Receptor beta Regulates Acyl-CoA Synthetase 2 in Reaggregated Rat Brain Cell Cultures J. Biol. Chem., December 10, 1999; 274(50): 35881 - 35888. [Abstract] [Full Text] [PDF]

				C. E. Gargiulo, S. M. Stuhlsatz-Krouper, and J. E. Schaffer Localization of adipocyte long-chain fatty acyl-CoA synthetase at the plasma membrane J. Lipid Res., May 1, 1999; 40(5): 881 - 892. [Abstract] [Full Text]

				A. Guilherme, M. Emoto, J. M. Buxton, S. Bose, R. Sabini, W. E. Theurkauf, J. Leszyk, and M. P. Czech Perinuclear Localization and Insulin Responsiveness of GLUT4 Requires Cytoskeletal Integrity in 3T3-L1 Adipocytes J. Biol. Chem., December 1, 2000; 275(49): 38151 - 38159. [Abstract] [Full Text] [PDF]

				T. M. Lewin, J.-H. Kim, D. A. Granger, J. E. Vance, and R. A. Coleman Acyl-CoA Synthetase Isoforms 1, 4, and 5 Are Present in Different Subcellular Membranes in Rat Liver and Can Be Inhibited Independently J. Biol. Chem., June 29, 2001; 276(27): 24674 - 24679. [Abstract] [Full Text] [PDF]