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J Biol Chem, Vol. 273, Issue 6, 3253-3256, February 6, 1998
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From the Departments of A yeast open reading frame sharing homology with
the two known yeast Rab GTPase-activating proteins (GAPs), Gyp6p and
Gyp7p, was found in a data base search. We have named the gene
containing this open reading frame GYP1. Recombinant Gyp1p
showed GAP activity on Sec4p, increasing both its steady-state rate and
single turnover GTPase activity. Gyp1p also stimulated the GTPase
activity of several other yeast Rab proteins including Ypt1p, Ypt7p,
and Ypt51p but showed no GAP activity on Ypt6p and Ypt32p. Deletion of
the GYP1 gene or overexpression of Gyp1p did not alter the
growth rate of yeast. However, overexpression of Gyp1p was inhibitory in combination with a subset of secretory mutants including
sec4-8 and several ypt1 mutants. This effect is
probably due to the increase in GAP activity, which can be observed in
a lysate from cells overexpressing Gyp1p. The finding that yeast Rab
GAPs share homology with proteins in other species, such as
Caenorhabditis elegans and human, suggests the existence of
a conserved Rab GAP family.
Cell Biology and
§ Molecular Biophysics and Biochemistry, Yale University
School of Medicine, New Haven, Connecticut 06520
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