| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 6, 3551-3561, February 6, 1998
From the The alveolar macrophage-derived peptide tumor
necrosis factor-
A Promoter Recruitment Mechanism for Tumor Necrosis
Factor-
-induced Interleukin-8 Transcription in Type II Pulmonary
Epithelial Cells
DEPENDENCE ON NUCLEAR ABUNDANCE OF Rel A, NF-
B1, AND c-Rel
TRANSCRIPTION FACTORS
,,,, and Department of Medicine and Sealy Center for
Molecular Science and the ¶ Department of Pediatrics,
University of Texas Medical Branch, Galveston, Texas 77555-1060
(TNF) initiates pulmonary inflammation through
its ability to stimulate interleukin-8 (IL-8) synthesis in alveolar
epithelial cells through an incompletely described transcriptional
mechanism. In this study, we use the technique of ligation-mediated
polymerase chain reaction (LMPCR) to record changes in transcription
factor occupancy of the IL-8 promoter after TNF stimulation of A549 human alveolar cells. Using dimethylsulfate/LMPCR, no detectable proteins bind the TATA box in unstimulated cells. By contrast, TNF
rapidly induces protection of G residues at 
79 and 80 coincident with endogenous IL-8 gene transcription. Using DNase I/LMPCR, we
observe inducible protection of nucleotides 60 to 99 (the TNF
response element) and nucleotides 3 to 32 (containing the TATA
box). Surprisingly, extensive TATA box protection is only seen after
TNF stimulation. Using a two-step microaffinity isolation/Western immunoblot DNA binding assay, we observe that the NF-B subunits Rel
A, NF-B1, and c-Rel inducibly bind the TNF response element; these
proteins undergo rapid TNF-inducible increases in nuclear abundance as a consequence of IB proteolysis. Furthermore, the peptide aldehyde N-acetyl-Leu-Leu-norleucinal, an agent
that blocks both IB proteolysis and NF-B subunit
translocation, abrogates recombinant human TNF-inducible IL-8 gene
transcription. These studies demonstrate that IL-8 is activated by a
promoter recruitment mechanism in alveolar epithelial cells, where
NF-B subunit translocation is required for (and coincident with)
binding of the constitutively active TATA box-binding proteins.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Thapa, J. S. Lee, S.-Y. Park, Y.-H. Bae, S.-K. Bae, J. B. Kwon, K.-J. Kim, M.-K. Kwak, Y.-J. Park, H. G. Choi, et al. Clotrimazole Ameliorates Intestinal Inflammation and Abnormal Angiogenesis by Inhibiting Interleukin-8 Expression through a Nuclear Factor-{kappa}B-Dependent Manner J. Pharmacol. Exp. Ther., November 1, 2008; 327(2): 353 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Necela, W. Su, and E. A. Thompson Peroxisome Proliferator-activated Receptor {gamma} Down-regulates Follistatin in Intestinal Epithelial Cells through SP1 J. Biol. Chem., October 31, 2008; 283(44): 29784 - 29794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Bao, D. Kolli, T. Liu, Y. Shan, R. P. Garofalo, and A. Casola Human Metapneumovirus Small Hydrophobic Protein Inhibits NF-{kappa}B Transcriptional Activity J. Virol., August 15, 2008; 82(16): 8224 - 8229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kota, A. Sabbah, T. H. Chang, R. Harnack, Y. Xiang, X. Meng, and S. Bose Role of Human {beta}-Defensin-2 during Tumor Necrosis Factor-{alpha}/NF-{kappa}B-mediated Innate Antiviral Response against Human Respiratory Syncytial Virus J. Biol. Chem., August 15, 2008; 283(33): 22417 - 22429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Samten, J. C. Townsend, S. E. Weis, A. Bhoumik, P. Klucar, H. Shams, and P. F. Barnes CREB, ATF, and AP-1 Transcription Factors Regulate IFN-{gamma} Secretion by Human T Cells in Response to Mycobacterial Antigen J. Immunol., August 1, 2008; 181(3): 2056 - 2064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Nowak, B. Tian, M. Jamaluddin, I. Boldogh, L. A. Vergara, S. Choudhary, and A. R. Brasier RelA Ser276 Phosphorylation Is Required for Activation of a Subset of NF-{kappa}B-Dependent Genes by Recruiting Cyclin-Dependent Kinase 9/Cyclin T1 Complexes Mol. Cell. Biol., June 1, 2008; 28(11): 3623 - 3638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Mullan, M. Riley, D. Clarke, A. Tatler, A. Sutcliffe, A. J. Knox, and L. Pang {beta}-Tryptase Regulates IL-8 Expression in Airway Smooth Muscle Cells by a PAR-2-Independent Mechanism Am. J. Respir. Cell Mol. Biol., May 1, 2008; 38(5): 600 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lu, J. Y. Wu, E. J. Beswick, T. Ohno, S. Odenbreit, R. Haas, V. E. Reyes, M. Kita, D. Y. Graham, and Y. Yamaoka Functional and Intracellular Signaling Differences Associated with the Helicobacter pylori AlpAB Adhesin from Western and East Asian Strains J. Biol. Chem., March 2, 2007; 282(9): 6242 - 6254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. O'Hara, A. Bhattacharyya, R. C. Mifflin, M. F. Smith, K. A. Ryan, K. G.-E. Scott, M. Naganuma, A. Casola, T. Izumi, S. Mitra, et al. Interleukin-8 Induction by Helicobacter pylori in Gastric Epithelial Cells is Dependent on Apurinic/Apyrimidinic Endonuclease-1/Redox Factor-1 J. Immunol., December 1, 2006; 177(11): 7990 - 7999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Espinoza, F. Tenzin, A. O. Cecchi, Z. Chen, M. L. Witten, and M. E. Smulson Expression of JP-8-Induced Inflammatory Genes in AEII Cells Is Mediated by NF-{kappa}B and PARP-1 Am. J. Respir. Cell Mol. Biol., October 1, 2006; 35(4): 479 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Boodoo, E. W. Spannhake, J. D. Powell, and M. R. Horton Differential regulation of hyaluronan-induced IL-8 and IP-10 in airway epithelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L479 - L486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fragaki, C. Kileztky, C. Trentesaux, J.-M. Zahm, O. Bajolet, M. Johnson, and E. Puchelle Downregulation by a long-acting beta2-adrenergic receptor agonist and corticosteroid of Staphylococcus aureus-induced airway epithelial inflammatory mediator production Am J Physiol Lung Cell Mol Physiol, July 1, 2006; 291(1): L11 - L18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Wu, H. Lu, Y. Sun, D. Y. Graham, H. S. Cheung, and Y. Yamaoka Balance between Polyoma Enhancing Activator 3 and Activator Protein 1 Regulates Helicobacter pylori-Stimulated Matrix Metalloproteinase 1 Expression. Cancer Res., May 15, 2006; 66(10): 5111 - 5120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hai, M.-L. Yeung, T. G. Wood, Y. Wei, S. Yamaoka, Z. Gatalica, K.-T. Jeang, and A. R. Brasier An Alternative Splice Product of I{kappa}B Kinase (IKK{gamma}), IKK{gamma}-{Delta}, Differentially Mediates Cytokine and Human T-Cell Leukemia Virus Type 1 Tax-Induced NF-{kappa}B Activation J. Virol., May 1, 2006; 80(9): 4227 - 4241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Adam, K. K. Hansen, O. F. Astudillo, L. Coulon, F. Bex, X. Duhant, E. Jaumotte, M. D. Hollenberg, and A. Jacquet The House Dust Mite Allergen Der p 1, Unlike Der p 3, Stimulates the Expression of Interleukin-8 in Human Airway Epithelial Cells via a Proteinase-activated Receptor-2-independent Mechanism J. Biol. Chem., March 17, 2006; 281(11): 6910 - 6923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jamaluddin, S. Choudhary, S. Wang, A. Casola, R. Huda, R. P. Garofalo, S. Ray, and A. R. Brasier Respiratory Syncytial Virus-Inducible BCL-3 Expression Antagonizes the STAT/IRF and NF-{kappa}B Signaling Pathways by Inducing Histone Deacetylase 1 Recruitment to the Interleukin-8 Promoter J. Virol., December 15, 2005; 79(24): 15302 - 15313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kudo, H. Lu, J. Y. Wu, D. Y. Graham, A. Casola, and Y. Yamaoka Regulation of RANTES Promoter Activation in Gastric Epithelial Cells Infected with Helicobacter pylori Infect. Immun., November 1, 2005; 73(11): 7602 - 7612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Choudhary, S. Boldogh, R. Garofalo, M. Jamaluddin, and A. R. Brasier Respiratory Syncytial Virus Influences NF-{kappa}B-Dependent Gene Expression through a Novel Pathway Involving MAP3K14/NIK Expression and Nuclear Complex Formation with NF-{kappa}B2 J. Virol., July 15, 2005; 79(14): 8948 - 8959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Tian, D. E. Nowak, M. Jamaluddin, S. Wang, and A. R. Brasier Identification of Direct Genomic Targets Downstream of the Nuclear Factor-{kappa}B Transcription Factor Mediating Tumor Necrosis Factor Signaling J. Biol. Chem., April 29, 2005; 280(17): 17435 - 17448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Page, V. S. Hughes, K. K. Odoms, K. E. Dunsmore, and M. B. Hershenson German Cockroach Proteases Regulate Interleukin-8 Expression via Nuclear Factor for Interleukin-6 in Human Bronchial Epithelial Cells Am. J. Respir. Cell Mol. Biol., March 1, 2005; 32(3): 225 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Manna and G. T. Ramesh Interleukin-8 Induces Nuclear Transcription Factor-{kappa}B through a TRAF6-dependent Pathway J. Biol. Chem., February 25, 2005; 280(8): 7010 - 7021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Brasier, H. Spratt, Z. Wu, I. Boldogh, Y. Zhang, R. P. Garofalo, A. Casola, J. Pashmi, A. Haag, B. Luxon, et al. Nuclear Heat Shock Response and Novel Nuclear Domain 10 Reorganization in Respiratory Syncytial Virus-Infected A549 Cells Identified by High-Resolution Two-Dimensional Gel Electrophoresis J. Virol., November 1, 2004; 78(21): 11461 - 11476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Kokkinakis, X. Liu, S. Chada, M. M. Ahmed, M. M. Shareef, U. K. Singha, S. Yang, and J. Luo Modulation of Gene Expression in Human Central Nervous System Tumors under Methionine Deprivation-induced Stress Cancer Res., October 15, 2004; 64(20): 7513 - 7525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Sparkman and V. Boggaram Nitric oxide increases IL-8 gene transcription and mRNA stability to enhance IL-8 gene expression in lung epithelial cells Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L764 - L773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Wickremasinghe, L. H. Thomas, C. M. O'Kane, J. Uddin, and J. S. Friedland Transcriptional Mechanisms Regulating Alveolar Epithelial Cell-specific CCL5 Secretion in Pulmonary Tuberculosis J. Biol. Chem., June 25, 2004; 279(26): 27199 - 27210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Antonicelli, D. Brown, M. Parmentier, E. M. Drost, N. Hirani, I. Rahman, K. Donaldson, and W. MacNee Regulation of LPS-mediated inflammation in vivo and in vitro by the thiol antioxidant Nacystelyn Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1319 - L1327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Balasubramanian, R. K. Ganju, and J. E. Groopman Hepatitis C Virus and HIV Envelope Proteins Collaboratively Mediate Interleukin-8 Secretion through Activation of p38 MAP Kinase and SHP2 in Hepatocytes J. Biol. Chem., September 12, 2003; 278(37): 35755 - 35766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Zhu, D. A. Bradbury, L. Pang, and A. J. Knox Transcriptional Regulation of Interleukin (IL)-8 by Bradykinin in Human Airway Smooth Muscle Cells Involves Prostanoid-dependent Activation of AP-1 and Nuclear Factor (NF)-IL-6 and Prostanoid-independent Activation of NF-{kappa}B J. Biol. Chem., August 1, 2003; 278(31): 29366 - 29375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Levine, J. A. Lucci III, B. Pazdrak, J.-Z. Cheng, Y.-S. Guo, C. M. Townsend Jr., and M. R. Hellmich Bombesin Stimulates Nuclear Factor {kappa}B Activation and Expression of Proangiogenic Factors in Prostate Cancer Cells Cancer Res., July 1, 2003; 63(13): 3495 - 3502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Young and G. D. Parks Simian Virus 5 Is a Poor Inducer of Chemokine Secretion from Human Lung Epithelial Cells: Identification of Viral Mutants That Activate Interleukin-8 Secretion by Distinct Mechanisms J. Virol., June 15, 2003; 77(12): 7124 - 7130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhou, A. Tan, S. Iasvovskaia, J. Li, A. Lin, and M. B. Hershenson Ras and Mitogen-Activated Protein Kinase Kinase Kinase-1 Coregulate Activator Protein-1- and Nuclear Factor-{kappa}B-Mediated Gene Expression in Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., June 1, 2003; 28(6): 762 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Page, J. Li, L. Zhou, S. Iasvoyskaia, K. C. Corbit, J.-W. Soh, I. B. Weinstein, A. R. Brasier, A. Lin, and M. B. Hershenson Regulation of Airway Epithelial Cell NF-{kappa}B-Dependent Gene Expression by Protein Kinase C{delta} J. Immunol., June 1, 2003; 170(11): 5681 - 5689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, M. Jamaluddin, S. Wang, B. Tian, R. P. Garofalo, A. Casola, and A. R. Brasier Ribavirin Treatment Up-Regulates Antiviral Gene Expression via the Interferon-Stimulated Response Element in Respiratory Syncytial Virus-Infected Epithelial Cells J. Virol., May 15, 2003; 77(10): 5933 - 5947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Tian and A. R. Brasier Identification of a Nuclear Factor Kappa B-dependent Gene Network Recent Prog. Horm. Res., January 1, 2003; 58(1): 95 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Casola, A. Henderson, T. Liu, R. P. Garofalo, and A. R. Brasier Regulation of RANTES promoter activation in alveolar epithelial cells after cytokine stimulation Am J Physiol Lung Cell Mol Physiol, December 1, 2002; 283(6): L1280 - L1290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hoffmann, O. Dittrich-Breiholz, H. Holtmann, and M. Kracht Multiple control of interleukin-8 gene expression J. Leukoc. Biol., November 1, 2002; 72(5): 847 - 855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, S. Kartha, S. Iasvovskaia, A. Tan, R. K. Bhat, J. M. Manaligod, K. Page, A. R. Brasier, and M. B. Hershenson Regulation of human airway epithelial cell IL-8 expression by MAP kinases Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L690 - L699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Cummings, N. L. Parinandi, A. Zaiman, L. Wang, P. V. Usatyuk, J. G. N. Garcia, and V. Natarajan Phospholipase D Activation by Sphingosine 1-Phosphate Regulates Interleukin-8 Secretion in Human Bronchial Epithelial Cells J. Biol. Chem., August 9, 2002; 277(33): 30227 - 30235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Pazdrak, B. Olszewska-Pazdrak, T. Liu, R. Takizawa, A. R. Brasier, R. P. Garofalo, and A. Casola MAPK activation is involved in posttranscriptional regulation of RSV-induced RANTES gene expression Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L364 - L372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Tian, Y. Zhang, B. A. Luxon, R. P. Garofalo, A. Casola, M. Sinha, and A. R. Brasier Identification of NF-{kappa}B-Dependent Gene Networks in Respiratory Syncytial Virus-Infected Cells J. Virol., June 5, 2002; 76(13): 6800 - 6814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Graness, C. E. Chwieralski, D. Reinhold, L. Thim, and W. Hoffmann Protein Kinase C and ERK Activation Are Required for TFF- peptide-stimulated Bronchial Epithelial Cell Migration and Tumor Necrosis Factor-alpha -induced Interleukin-6 (IL-6) and IL-8 Secretion J. Biol. Chem., May 17, 2002; 277(21): 18440 - 18446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fujiie, K. Hieshima, D. Izawa, T. Nakayama, R. Fujisawa, H. Ohyanagi, and O. Yoshie Proinflammatory cytokines induce liver and activation-regulated chemokine/macrophage inflammatory protein-3{alpha}/CCL20 in mucosal epithelial cells through NF-{kappa}B Int. Immunol., October 1, 2001; 13(10): 1255 - 1263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yeh, N. Leitinger, R. de Martin, N. Onai, K. Matsushima, D. K. Vora, J. A. Berliner, and S. T. Reddy Increased Transcription of IL-8 in Endothelial Cells Is Differentially Regulated by TNF-{alpha} and Oxidized Phospholipids Arterioscler. Thromb. Vasc. Biol., October 1, 2001; 21(10): 1585 - 1591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Casola, R. P. Garofalo, H. Haeberle, T. F. Elliott, R. Lin, M. Jamaluddin, and A. R. Brasier Multiple cis Regulatory Elements Control RANTES Promoter Activity in Alveolar Epithelial Cells Infected with Respiratory Syncytial Virus J. Virol., July 15, 2001; 75(14): 6428 - 6439. [Abstract] [Full Text]
|
|
|
|
|
|
C. Zouki, L. József, S. Ouellet, Y. Paquette, and J. G. Filep Peroxynitrite mediates cytokine-induced IL-8 gene expression and production by human leukocytes J. Leukoc. Biol., May 1, 2001; 69(5): 815 - 824. [Abstract] [Full Text]
|
|
|
|
|
|
N.-H. Cho, S.-Y. Seong, M.-S. Choi, and I.-S. Kim Expression of Chemokine Genes in Human Dermal Microvascular Endothelial Cell Lines Infected with Orientia tsutsugamushi Infect. Immun., March 1, 2001; 69(3): 1265 - 1272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jamaluddin, S. Wang, R. P. Garofalo, T. Elliott, A. Casola, S. Baron, and A. R. Brasier IFN-{beta} mediates coordinate expression of antigen-processing genes in RSV-infected pulmonary epithelial cells Am J Physiol Lung Cell Mol Physiol, February 1, 2001; 280(2): L248 - L257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Thomas, M. I. Y. Wickremasinghe, M. Sharland, and J. S. Friedland Synergistic Upregulation of Interleukin-8 Secretion from Pulmonary Epithelial Cells by Direct and Monocyte-Dependent Effects of Respiratory Syncytial Virus Infection J. Virol., September 15, 2000; 74(18): 8425 - 8433. [Abstract] [Full Text]
|
|
|
|
|
|
D. J. Philpott, S. Yamaoka, A. Israel, and P. J. Sansonetti Invasive Shigella flexneri Activates NF-{kappa}B Through a Lipopolysaccharide-Dependent Innate Intracellular Response and Leads to IL-8 Expression in Epithelial Cells J. Immunol., July 15, 2000; 165(2): 903 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pang and A. J. Knox Synergistic Inhibition by beta 2-Agonists and Corticosteroids on Tumor Necrosis Factor-alpha -Induced Interleukin-8 Release from Cultured Human Airway Smooth-Muscle Cells Am. J. Respir. Cell Mol. Biol., July 1, 2000; 23(1): 79 - 85. [Abstract] [Full Text]
|
|
|
|
|
|
A. Casola, R. P. Garofalo, M. Jamaluddin, S. Vlahopoulos, and A. R. Brasier Requirement of a Novel Upstream Response Element in Respiratory Syncytial Virus-Induced IL-8 Gene Expression J. Immunol., June 1, 2000; 164(11): 5944 - 5951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jamaluddin, T. Meng, J. Sun, I. Boldogh, Y. Han, and A. R. Brasier Angiotensin II Induces Nuclear Factor (NF)-{kappa}B1 Isoforms to Bind the Angiotensinogen Gene Acute-Phase Response Element: A Stimulus-Specific Pathway for NF-{kappa}B Activation Mol. Endocrinol., January 1, 2000; 14(1): 99 - 113. [Abstract] [Full Text]
|
|
|
|
|
|
H. Holtmann, R. Winzen, P. Holland, S. Eickemeier, E. |
