| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 6, 3582-3587, February 6, 1998
From the Vesicles containing the glucose transporter GLUT4
from rat adipocytes contain a major protein of 110 kDa. We have
isolated this protein, obtained the sequences of peptides, and cloned a large portion of its cDNA. This revealed that the protein is
sortilin, a novel membrane protein that was cloned in another context
from a human source while this work was in progress. Subcellular
fractionation of rat and 3T3-L1 adipocytes, together with GLUT4 vesicle
isolation, showed that sortilin was primarily located in the low
density microsomes in vesicles containing GLUT4. Insulin caused a
1.7-fold increase in the amount of sortilin at the plasma membranes of 3T3-L1 adipocytes, as assessed by cell surface biotinylation. The
expression of sortilin in 3T3-L1 cells occurred only upon differentiation. Previous characterization of sortilin has led to the
suggestion that it functions to sort lumenal proteins from the trans
Golgi. The significance of its insulin-stimulated increase at the cell
surface and of its expression upon differentiation will require
definitive delineation of its function.
Sortilin Is the Major 110-kDa Protein in GLUT4 Vesicles from
Adipocytes
,,
,,, and
Department of Biochemistry, Dartmouth
Medical School, Hanover, New Hampshire 03755, the ¶ Harvard
Microchemistry Facility, Department of Molecular and Cellular Biology,
Harvard University, Cambridge, Massachusetts 02138, and the
Department of Medical Biochemistry, University of Aarhus, 8000 Aarhus C, Denmark
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Shi, G. Huang, and K. V. Kandror Self-assembly of Glut4 Storage Vesicles during Differentiation of 3T3-L1 Adipocytes J. Biol. Chem., October 31, 2008; 283(44): 30311 - 30321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ariga, T. Nedachi, H. Katagiri, and M. Kanzaki Functional Role of Sortilin in Myogenesis and Development of Insulin-responsive Glucose Transport System in C2C12 Myocytes J. Biol. Chem., April 11, 2008; 283(15): 10208 - 10220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Muretta, I. Romenskaia, P. A. Cassiday, and C. C. Mastick Expression of a synapsin IIb site 1 phosphorylation mutant in 3T3-L1 adipocytes inhibits basal intracellular retention of Glut4 J. Cell Sci., April 1, 2007; 120(7): 1168 - 1177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shi and K. V. Kandror The Luminal Vps10p Domain of Sortilin Plays the Predominant Role in Targeting to Insulin-responsive Glut4-containing Vesicles J. Biol. Chem., March 23, 2007; 282(12): 9008 - 9016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Larance, G. Ramm, J. Stockli, E. M. van Dam, S. Winata, V. Wasinger, F. Simpson, M. Graham, J. R. Junutula, M. Guilhaus, et al. Characterization of the Role of the Rab GTPase-activating Protein AS160 in Insulin-regulated GLUT4 Trafficking J. Biol. Chem., November 11, 2005; 280(45): 37803 - 37813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Eriste, A. Norberg, D. Nepomuceno, C. Kuei, F. Kamme, D.-T. Tran, K. Strupat, H. Jornvall, C. Liu, T. W. Lovenberg, et al. A Novel Form of Neurotensin Post-translationally Modified by Arginylation J. Biol. Chem., October 21, 2005; 280(42): 35089 - 35097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. V. Li and K. V. Kandror Golgi-Localized, {gamma}-Ear-Containing, Arf-Binding Protein Adaptors Mediate Insulin-Responsive Trafficking of Glucose Transporter 4 in 3T3-L1 Adipocytes Mol. Endocrinol., August 1, 2005; 19(8): 2145 - 2153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hosaka, C. C. Brooks, E. Presman, S.-K. Kim, Z. Zhang, M. Breen, D. N. Gross, E. Sztul, and P. F. Pilch p115 Interacts with the GLUT4 Vesicle Protein, IRAP, and Plays a Critical Role in Insulin-stimulated GLUT4 Translocation Mol. Biol. Cell, June 1, 2005; 16(6): 2882 - 2890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. H. Suh, Y. Kim, J. H. Bang, K. S. Choi, J. W. Lee, W.-H. Kim, T. J. Oh, S. An, and M. H. Jung Analysis of gene expression profiles in insulin-sensitive tissues from pre-diabetic and diabetic Zucker diabetic fatty rats J. Mol. Endocrinol., April 1, 2005; 34(2): 299 - 315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Conticello, N. D. Kowalsman, C. Jacobsen, G. Yudkovsky, K. Sato, Z. Elazar, C. M. Petersen, A. Aronheim, and M. Fainzilber The Prodomain of a Secreted Hydrophobic Mini-protein Facilitates Its Export from the Endoplasmic Reticulum by Hitchhiking on Sorting Receptors J. Biol. Chem., July 11, 2003; 278(29): 26311 - 26314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. K. I. Perera, M. Clarke, N. J. Morris, W. Hong, L. H. Chamberlain, and G. W. Gould Syntaxin 6 Regulates Glut4 Trafficking in 3T3-L1 Adipocytes Mol. Biol. Cell, July 1, 2003; 14(7): 2946 - 2958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sarret, L. Gendron, P. Kilian, H. M. K. Nguyen, N. Gallo-Payet, M.-D. Payet, and A. Beaudet Pharmacology and Functional Properties of NTS2 Neurotensin Receptors in Cerebellar Granule Cells J. Biol. Chem., September 20, 2002; 277(39): 36233 - 36243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zeigerer, M. A. Lampson, O. Karylowski, D. D. Sabatini, M. Adesnik, M. Ren, and T. E. McGraw GLUT4 Retention in Adipocytes Requires Two Intracellular Insulin-regulated Transport Steps Mol. Biol. Cell, July 1, 2002; 13(7): 2421 - 2435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kane, H. Sano, S. C. H. Liu, J. M. Asara, W. S. Lane, C. C. Garner, and G. E. Lienhard A Method to Identify Serine Kinase Substrates. Akt PHOSPHORYLATES A NOVEL ADIPOCYTE PROTEIN WITH A Rab GTPASE-ACTIVATING PROTEIN (GAP) DOMAIN J. Biol. Chem., June 14, 2002; 277(25): 22115 - 22118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. B. Binder, B. Kinkead, M. J. Owens, and C. B. Nemeroff Neurotensin and Dopamine Interactions Pharmacol. Rev., December 1, 2001; 53(4): 453 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Boman GGA proteins: new players in the sorting game J. Cell Sci., January 10, 2001; 114(19): 3413 - 3418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zhou, G. Vallega, K. V. Kandror, and P. F. Pilch Insulin-mediated translocation of GLUT-4-containing vesicles is preserved in denervated muscles Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E1019 - E1026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hashiramoto and D. E. James Characterization of Insulin-Responsive GLUT4 Storage Vesicles Isolated from 3T3-L1 Adipocytes Mol. Cell. Biol., January 1, 2000; 20(1): 416 - 427. [Abstract] [Full Text]
|
|
|
|
|
|
A. K. El-Jack, K. V. Kandror, and P. F. Pilch The Formation of an Insulin-responsive Vesicular Cargo Compartment Is an Early Event in 3T3-L1 Adipocyte Differentiation Mol. Biol. Cell, May 1, 1999; 10(5): 1581 - 1594. [Abstract] [Full Text]
|
|
|
|
|
|
M. S. Nielsen, C. Jacobsen, G. Olivecrona, J. Gliemann, and C. M. Petersen Sortilin/Neurotensin Receptor-3 Binds and Mediates Degradation of Lipoprotein Lipase J. Biol. Chem., March 26, 1999; 274(13): 8832 - 8836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Kupriyanova and K. V. Kandror Akt-2 Binds to Glut4-containing Vesicles and Phosphorylates Their Component Proteins in Response to Insulin J. Biol. Chem., January 15, 1999; 274(3): 1458 - 1464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mazella, N. Zsurger, V. Navarro, J. Chabry, M. Kaghad, D. Caput, P. Ferrara, N. Vita, D. Gully, J.-P. Maffrand, et al. The 100-kDa Neurotensin Receptor Is gp95/Sortilin, A Non-G-Protein-coupled Receptor J. Biol. Chem., October 9, 1998; 273(41): 26273 - 26276. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
