| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 6, 3679-3686, February 6, 1998
From the Hop is a 60-kDa protein characterized by its
ability to bind the two chaperones, hsp70 and hsp90. We have tested the
function of Hop using an assay for the refolding of denatured firefly
luciferase. We show that Hop is involved in the process of refolding
thermally denatured firefly luciferase in rabbit reticulocyte lysate.
Hop also stimulates refolding by hsp70 and Ydj-1 in a purified
refolding system. Hsp90 can also stimulate refolding, and optimal
refolding is observed in the presence of both Hop and hsp90. Similar
stimulation was observed when Hop was replaced by its yeast homolog
Sti1. In assays of the binding of Hop to hsp70 and hsp90, Hop
preferentially forms a complex with ADP-bound hsp70, and this process
is unaffected by the presence of hsp90. Hop does not alter the ATPase
activity or the rate of ADP dissociation of hsp70. Hop also appears to bind to the ADP-bound form of hsp90, blocking the
ATP-dependent conversion of hsp90 to a form capable of
interacting with p23. Conversely, once p23 is bound to hsp90, Hop
binding is diminished. These results confirm that Hop provides a
physical link between hsp70 and hsp90 and also indicate that Hop
modulates the activities of both of these chaperone proteins.
Hop Modulates hsp70/hsp90 Interactions in Protein Folding
,, and
Department of Biochemistry and Molecular
Biology, Mayo Graduate School, Rochester, Minnesota 55905 and the
§ Department of Genetics and Howard Hughes Medical
Institute, Yale University School of Medicine,
New Haven, Connecticut 06510
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Shimamoto, M. Takata, M. Tokuda, F. Oohira, H. Tokumitsu, and R. Kobayashi Interactions of S100A2 and S100A6 with the Tetratricopeptide Repeat Proteins, Hsp90/Hsp70-organizing Protein and Kinesin Light Chain J. Biol. Chem., October 17, 2008; 283(42): 28246 - 28258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J R Jenkins A proteomic approach to identifying new drug targets (potentiating topoisomerase II poisons) Br. J. Radiol., October 1, 2008; 81(Special_Issue_1): S69 - S77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Holmes, S. Y. Sharp, S. Hobbs, and P. Workman Silencing of HSP90 Cochaperone AHA1 Expression Decreases Client Protein Activation and Increases Cellular Sensitivity to the HSP90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin Cancer Res., February 15, 2008; 68(4): 1188 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bedard, S. Kubis, S. Bimanadham, and P. Jarvis Functional Similarity between the Chloroplast Translocon Component, Tic40, and the Human Co-chaperone, Hsp70-interacting Protein (Hip) J. Biol. Chem., July 20, 2007; 282(29): 21404 - 21414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Alkhalfioui, M. Renard, W. H. Vensel, J. Wong, C. K. Tanaka, W. J. Hurkman, B. B. Buchanan, and F. Montrichard Thioredoxin-Linked Proteins Are Reduced during Germination of Medicago truncatula Seeds Plant Physiology, July 1, 2007; 144(3): 1559 - 1579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Catlett and K. B. Kaplan Sgt1p Is a Unique Co-chaperone That Acts as a Client Adaptor to Link Hsp90 to Skp1p J. Biol. Chem., November 3, 2006; 281(44): 33739 - 33748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Cintron and D. Toft Defining the Requirements for Hsp40 and Hsp70 in the Hsp90 Chaperone Pathway J. Biol. Chem., September 8, 2006; 281(36): 26235 - 26244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Song and D. C. Masison Independent Regulation of Hsp70 and Hsp90 Chaperones by Hsp70/Hsp90-organizing Protein Sti1 (Hop1) J. Biol. Chem., October 7, 2005; 280(40): 34178 - 34185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Carbone, J. A. Doorn, Z. Kiebler, B. R. Ickes, and D. R. Petersen Modification of Heat Shock Protein 90 by 4-Hydroxynonenal in a Rat Model of Chronic Alcoholic Liver Disease J. Pharmacol. Exp. Ther., October 1, 2005; 315(1): 8 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Adjei and M. Hidalgo Intracellular Signal Transduction Pathway Proteins As Targets for Cancer Therapy J. Clin. Oncol., August 10, 2005; 23(23): 5386 - 5403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. De Nardo, P. Masendycz, S. Ho, M. Cross, A. J. Fleetwood, E. C. Reynolds, J. A. Hamilton, and G. M. Scholz A Central Role for the Hsp90{middle dot}Cdc37 Molecular Chaperone Module in Interleukin-1 Receptor-associated-kinase-dependent Signaling by Toll-like Receptors J. Biol. Chem., March 18, 2005; 280(11): 9813 - 9822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Youker, P. Walsh, T. Beilharz, T. Lithgow, and J. L. Brodsky Distinct Roles for the Hsp40 and Hsp90 Molecular Chaperones during Cystic Fibrosis Transmembrane Conductance Regulator Degradation in Yeast Mol. Biol. Cell, November 1, 2004; 15(11): 4787 - 4797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Prince and R. L. Matts Definition of Protein Kinase Sequence Motifs That Trigger High Affinity Binding of Hsp90 and Cdc37 J. Biol. Chem., September 17, 2004; 279(38): 39975 - 39981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Carrigan, G. M. Nelson, P. J. Roberts, J. Stoffer, D. L. Riggs, and D. F. Smith Multiple Domains of the Co-chaperone Hop Are Important for Hsp70 Binding J. Biol. Chem., April 16, 2004; 279(16): 16185 - 16193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Cortajarena, T. Kajander, W. Pan, M. J. Cocco, and L. Regan Protein design to understand peptide ligand recognition by tetratricopeptide repeat proteins Protein Eng. Des. Sel., April 1, 2004; 17(4): 399 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wegele, P. Muschler, M. Bunck, J. Reinstein, and J. Buchner Dissection of the Contribution of Individual Domains to the ATPase Mechanism of Hsp90 J. Biol. Chem., October 10, 2003; 278(41): 39303 - 39310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wegele, M. Haslbeck, J. Reinstein, and J. Buchner Sti1 Is a Novel Activator of the Ssa Proteins J. Biol. Chem., July 3, 2003; 278(28): 25970 - 25976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. Lotz, H. Lin, A. Harst, and W. M. J. Obermann Aha1 Binds to the Middle Domain of Hsp90, Contributes to Client Protein Activation, and Stimulates the ATPase Activity of the Molecular Chaperone J. Biol. Chem., May 2, 2003; 278(19): 17228 - 17235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Vignols, N. Mouaheb, D. Thomas, and Y. Meyer Redox Control of Hsp70-Co-chaperone Interaction Revealed by Expression of a Thioredoxin-like Arabidopsis Protein J. Biol. Chem., February 7, 2003; 278(7): 4516 - 4523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Lavictoire, D. A. E. Parolin, A. C. Klimowicz, J. F. Kelly, and I. A. J. Lorimer Interaction of Hsp90 with the Nascent Form of the Mutant Epidermal Growth Factor Receptor EGFRvIII J. Biol. Chem., February 7, 2003; 278(7): 5292 - 5299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. B. Pratt and D. O. Toft Regulation of Signaling Protein Function and Trafficking by the hsp90/hsp70-Based Chaperone Machinery Experimental Biology and Medicine, February 1, 2003; 228(2): 111 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, M. K. Quick, K. C. Kanelakis, M. Gijzen, and P. Krishna Characterization of a Plant Homolog of Hop, a Cochaperone of Hsp90 Plant Physiology, February 1, 2003; 131(2): 525 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Chung, Y. Shen, and L. M. Hendershot BAP, a Mammalian BiP-associated Protein, Is a Nucleotide Exchange Factor That Regulates the ATPase Activity of BiP J. Biol. Chem., November 27, 2002; 277(49): 47557 - 47563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Hernandez, W. P. Sullivan, and D. O. Toft The Assembly and Intermolecular Properties of the hsp70-Hop-hsp90 Molecular Chaperone Complex J. Biol. Chem., October 4, 2002; 277(41): 38294 - 38304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Kanelakis, D. S. Shewach, and W. B. Pratt Nucleotide Binding States of hsp70 and hsp90 during Sequential Steps in the Process of Glucocorticoid Receptor{middle dot}hsp90 Heterocomplex Assembly J. Biol. Chem., September 6, 2002; 277(37): 33698 - 33703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Siligardi, B. Panaretou, P. Meyer, S. Singh, D. N. Woolfson, P. W. Piper, L. H. Pearl, and C. Prodromou Regulation of Hsp90 ATPase Activity by the Co-chaperone Cdc37p/p50cdc37 J. Biol. Chem., May 31, 2002; 277(23): 20151 - 20159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Hernandez, A. Chadli, and D. O. Toft HSP40 Binding Is the First Step in the HSP90 Chaperoning Pathway for the Progesterone Receptor J. Biol. Chem., March 29, 2002; 277(14): 11873 - 11881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. L. Owen, W. P. Sullivan, S. J. Felts, and D. O. Toft Regulation of Heat Shock Protein 90 ATPase Activity by Sequences in the Carboxyl Terminus J. Biol. Chem., February 22, 2002; 277(9): 7086 - 7091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Young, I. Moarefi, and F. U. Hartl Hsp90: a specialized but essential protein-folding tool J. Cell Biol., July 23, 2001; 154(2): 267 - 274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chadli, I. Bouhouche, W. Sullivan, B. Stensgard, N. McMahon, M. G. Catelli, and D. O. Toft Dimerization and N-terminal domain proximity underlie the function of the molecular chaperone heat shock protein 90 PNAS, October 23, 2000; (2000) 220430297. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Valen, T. Kawakami, P. Tahepold, A. Dumitrescu, C. Lowbeer, and J. Vaage Glucocorticoid pretreatment protects cardiac function and induces cardiac heat shock protein 72 Am J Physiol Heart Circ Physiol, August 1, 2000; 279(2): H836 - H843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lewis, A. Devin, A. Miller, Y. Lin, Y. Rodriguez, L. Neckers, and Z.-g. Liu Disruption of Hsp90 Function Results in Degradation of the Death Domain Kinase, Receptor-interacting Protein (RIP), and Blockage of Tumor Necrosis Factor-induced Nuclear Factor-kappa B Activation J. Biol. Chem., March 31, 2000; 275(14): 10519 - 10526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Morishima, K. C. Kanelakis, A. M. Silverstein, K. D. Dittmar, L. Estrada, and W. B. Pratt The Hsp Organizer Protein Hop Enhances the Rate of but Is Not Essential for Glucocorticoid Receptor Folding by the Multiprotein Hsp90-based Chaperone System J. Biol. Chem., March 15, 2000; 275(10): 6894 - 6900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Felts, B. A. L. Owen, P. Nguyen, J. Trepel, D. B. Donner, and D. O. Toft The hsp90-related Protein TRAP1 Is a Mitochondrial Protein with Distinct Functional Properties J. Biol. Chem., February 4, 2000; 275(5): 3305 - 3312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Lee and E. Vierling A Small Heat Shock Protein Cooperates with Heat Shock Protein 70 Systems to Reactivate a Heat-Denatured Protein Plant Physiology, January 1, 2000; 122(1): 189 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Michels, B. Kanon, O. Bensaude, and H. H. Kampinga Heat Shock Protein (Hsp) 40 Mutants Inhibit Hsp70 in Mammalian Cells J. Biol. Chem., December 17, 1999; 274(51): 36757 - 36763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Kanelakis, Y. Morishima, K. D. Dittmar, M. D. Galigniana, S. Takayama, J. C. Reed, and W. B. Pratt Differential Effects of the hsp70-binding Protein BAG-1 on Glucocorticoid Receptor Folding by the hsp90-based Chaperone Machinery J. Biol. Chem., November 26, 1999; 274(48): 34134 - 34140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Schneikert, S. Hubner, E. Martin, and A. C.B. Cato A Nuclear Action of the Eukaryotic Cochaperone RAP46 in Downregulation of Glucocorticoid Receptor Activity J. Cell Biol., September 6, 1999; 146(5): 929 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Heine, R. L. Delude, B. G. Monks, T. Espevik, and D. T. Golenbock Bacterial Lipopolysaccharide Induces Expression of the Stress Response Genes hop and H411 J. Biol. Chem., July 23, 1999; 274(30): 21049 - 21055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Grenert, B. D. Johnson, and D. O. Toft The Importance of ATP Binding and Hydrolysis by Hsp90 in Formation and Function of Protein Heterocomplexes J. Biol. Chem., June 18, 1999; 274(25): 17525 - 17533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Fink Chaperone-Mediated Protein Folding Physiol Rev, April 1, 1999; 79(2): 425 - 449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Carrello, E. Ingley, R. F. Minchin, S. Tsai, and T. Ratajczak The Common Tetratricopeptide Repeat Acceptor Site for Steroid Receptor-associated Immunophilins and Hop Is Located in the Dimerization Domain of Hsp90 J. Biol. Chem., January 29, 1999; 274(5): 2682 - 2689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gross, S. Hessefort, and A. Olin Purification of a 38-kDa Protein from Rabbit Reticulocyte Lysate Which Promotes Protein Renaturation by Heat Shock Protein 70 and Its Identification as delta -Aminolevulinic Acid Dehydratase and as a Putative DnaJ Protein J. Biol. Chem., January 29, 1999; 274(5): 3125 - 3134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chen and D. F. Smith Hop as an Adaptor in the Heat Shock Protein 70 (Hsp70) and Hsp90 Chaperone Machinery J. Biol. Chem., December 25, 1998; 273(52): 35194 - 35200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Raynes and V. Guerriero Jr. Inhibition of Hsp70 ATPase Activity and Protein Renaturation by a Novel Hsp70-binding Protein J. Biol. Chem., December 4, 1998; 273(49): 32883 - 32888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kosano, B. Stensgard, M. C. Charlesworth, N. McMahon, and D. Toft The Assembly of Progesterone Receptor-hsp90 Complexes Using Purified Proteins J. Biol. Chem., December 4, 1998; 273(49): 32973 - 32979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M.J. Obermann, H. Sondermann, A. A. Russo, N. P. Pavletich, and F. U. Hartl In Vivo Function of Hsp90 Is Dependent on ATP Binding and ATP Hydrolysis J. Cell Biol., November 16, 1998; 143(4): 901 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gebauer, R. Melki, and U. Gehring The Chaperone Cofactor Hop/p60 Interacts with the Cytosolic Chaperonin-containing TCP-1 and Affects Its Nucleotide Exchange and Protein Folding Activities J. Biol. Chem., November 6, 1998; 273(45): 29475 - 29480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gebauer, M. Zeiner, and U. Gehring Interference between Proteins Hap46 and Hop/p60, Which Bind to Different Domains of the Molecular Chaperone hsp70/hsc70 Mol. Cell. Biol., November 1, 1998; 18(11): 6238 - 6244. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Lu and D. M. Cyr Protein Folding Activity of Hsp70 Is Modified Differentially by the Hsp40 Co-chaperones Sis1 and Ydj1 J. Biol. Chem., October 23, 1998; 273(43): 27824 - 27830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Rajapandi, L. E. Greene, and E. Eisenberg The Molecular Chaperones Hsp90 and Hsc70 Are Both Necessary and Sufficient to Activate Hormone Binding by Glucocorticoid Receptor J. Biol. Chem., July 14, 2000; 275(29): 22597 - 22604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Johnson, A. Chadli, S. J. Felts, I. Bouhouche, M. G. Catelli, and D. O. Toft Hsp90 Chaperone Activity Requires the Full-length Protein and Interaction among Its Multiple Domains J. Biol. Chem., October 13, 2000; 275(42): 32499 - 32507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Horton, P. James, E. A. Craig, and J. O. Hensold The Yeast hsp70 Homologue Ssa Is Required for Translation and Interacts with Sis1 and Pab1 on Translating Ribosomes J. Biol. Chem., April 20, 2001; 276(17): 14426 - 14433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Scholz, K. Cartledge, and N. E. Hall Identification and Characterization of Harc, a Novel Hsp90-associating Relative of Cdc37 J. Biol. Chem., August 10, 2001; 276(33): 30971 - 30979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chadli, I. Bouhouche, W. Sullivan, B. Stensgard, N. McMahon, M. G. Catelli, and D. O. Toft Dimerization and N-terminal domain proximity underlie the function of the molecular chaperone heat shock protein 90 PNAS, November 7, 2000; 97(23): 12524 - 12529. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
