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J Biol Chem, Vol. 273, Issue 6, 3702-3711, February 6, 1998
From the Department of Physiological Chemistry,
Ruhr-Universität Bochum, 44780 Bochum, Germany
In Saccharomyces cerevisiae the
metabolic degradation of saturated fatty acids is exclusively confined
to peroxisomes. In addition to a functional
IDP3 Encodes a Peroxisomal NADP-dependent
Isocitrate Dehydrogenase Required for the
-Oxidation of
Unsaturated Fatty Acids
-oxidation system, the
degradation of unsaturated fatty acids requires auxiliary enzymes,
including a 
2,3-enoyl-CoA isomerase and an
NADPH-dependent 2,4-dienoyl-CoA reductase. We found both
enzymes to be present in yeast peroxisomes. The impermeability of the
peroxisomal membrane for pyrimidine nucleotides led to the question of
how the NADPH needed by the reductase is regenerated in the peroxisomal
lumen. We report the identification and functional analysis of the
IDP3 gene product, which is a yeast peroxisomal
NADP-dependent isocitrate dehydrogenase. The newly
identified peroxisomal protein is homologous to the mitochondrial Idp1p
and cytosolic Idp2p, which both are yeast NADP-dependent
isocitrate dehydrogenases. Yeast cells lacking Idp3p grow normally on
saturated fatty acids, but growth is impaired on unsaturated fatty
acids, indicating that the peroxisomal Idp3p is involved in their
metabolic utilization. The data presented are consistent with the
assumption that peroxisomes of S. cerevisiae contain
the enzyme equipment needed for the degradation of unsaturated fatty acids, including an NADP-dependent isocitrate
dehydrogenase, a putative constituent of a peroxisomal
NADPH-regenerating redox system.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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