| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 7, 3817-3829, February 13, 1998
,,, and
From the For the first time,
the complete structure of a lipopolysaccharide (LPS) core region from
Salmonella enterica has been identified that is different
from the Ra core type generally thought to be present in all
Salmonella LPS. The LPSs from two rough mutants and the
smooth form of S. enterica sv. Arizonae IIIa O62, which all
failed to react with an Ra core type-specific monoclonal antibody and
were resistant to phage FO1, were analyzed after chemical modification
using monosaccharide analysis, mass spectrometry, and NMR spectroscopy.
In the novel core type, the terminal D-GlcNAc residue
present in the Ra core type, is replaced by a D-Glc
residue. The O-specific polysaccharide is Department of Mass Spectrometry, Bijvoet
Center for Biomolecular Research, Utrecht University, NL-3508 TB
Utrecht, The Netherlands, the § Department of Chemistry,
Carlsberg Laboratory, DK-2500 Valby, Denmark, the
¶ Max-Planck-Institute for Immunobiology, D-79108 Freiburg,
Germany, and the Division of Medical and Biochemical
Microbiology, Center for Medicine and Biosciences, Research Center
Borstel, D-23845 Borstel, Germany
1
4-linked to the second
distal Glc residue of the core. Furthermore, phosphoryl substituents attached to O-4 of
L-glycero-D-manno-heptose
(Hep) I and II were identified as 2-aminoethyl diphosphate (on Hep I)
and phosphate (Hep
II).
Abbreviations
in Structure I are as follows: Hepp,
L-glycero-D-manno-heptopyranose;
Kdo, 3-deoxy-D-manno-oct-2-ulopyranosonic acid;
PPEA, 2-aminoethyl diphosphate; R, O-specific polysaccharide. The
presence of this novel core type in LPS of S. enterica
should be taken into account in the development of a general
antibody-based diagnostic system for
Salmonella.
This article has been cited by other articles:
|
|
 |
|
  M. D. Leipold, N. A. Kaniuk, and C. Whitfield The C-terminal Domain of the Escherichia coli WaaJ Glycosyltransferase Is Important for Catalytic Activity and Membrane Association J. Biol. Chem., January 12, 2007; 282(2): 1257 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Kaniuk, E. Vinogradov, and C. Whitfield Investigation of the Structural Requirements in the Lipopolysaccharide Core Acceptor for Ligation of O Antigens in the Genus Salmonella: WAAL "LIGASE" IS NOT THE SOLE DETERMINANT OF ACCEPTOR SPECIFICITY J. Biol. Chem., August 27, 2004; 279(35): 36470 - 36480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Kaniuk, E. Vinogradov, J. Li, M. A. Monteiro, and C. Whitfield Chromosomal and Plasmid-encoded Enzymes Are Required for Assembly of the R3-type Core Oligosaccharide in the Lipopolysaccharide of Escherichia coli O157:H7 J. Biol. Chem., July 23, 2004; 279(30): 31237 - 31250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Whitfield, N. Kaniuk, and E. Frirdich Molecular insights into the assembly and diversity of the outer core oligosaccharide in lipopolysaccharides from Escherichia coli and Salmonella Innate Immunity, August 1, 2003; 9(4): 244 - 249. [Abstract] [PDF]
|
|
|
|
|
|
S. Muller-Loennies, L. Brade, C. R. MacKenzie, F. E. Di Padova, and H. Brade Identification of a Cross-reactive Epitope Widely Present in Lipopolysaccharide from Enterobacteria and Recognized by the Cross-protective Monoclonal Antibody WN1 222-5 J. Biol. Chem., July 3, 2003; 278(28): 25618 - 25627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Niedziela, J. Lukasiewicz, W. Jachymek, M. Dzieciatkowska, C. Lugowski, and L. Kenne Core Oligosaccharides of Plesiomonas shigelloides O54:H2 (Strain CNCTC 113/92). STRUCTURAL AND SEROLOGICAL ANALYSIS OF THE LIPOPOLYSACCHARIDE CORE REGION, THE O-ANTIGEN BIOLOGICAL REPEATING UNIT, AND THE LINKAGE BETWEEN THEM J. Biol. Chem., March 29, 2002; 277(14): 11653 - 11663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. P. MANSFIELD and S.J. FORSYTHE Demonstration of the Rb1 lipopolysaccharide core structure in Salmonella strains with the monoclonal antibody M105 J. Med. Microbiol., April 1, 2001; 50(4): 339 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Yethon, J. S. Gunn, R. K. Ernst, S. I. Miller, L. Laroche, D. Malo, and C. Whitfield Salmonella enterica Serovar Typhimurium waaP Mutants Show Increased Susceptibility to Polymyxin and Loss of Virulence In Vivo Infect. Immun., August 1, 2000; 68(8): 4485 - 4491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Amor, D. E. Heinrichs, E. Frirdich, K. Ziebell, R. P. Johnson, and C. Whitfield Distribution of Core Oligosaccharide Types in Lipopolysaccharides from Escherichia coli Infect. Immun., March 1, 2000; 68(3): 1116 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Yethon, D. E. Heinrichs, M. A. Monteiro, M. B. Perry, and C. Whitfield Involvement of waaY, waaQ, and waaP in the Modification of Escherichia coli Lipopolysaccharide and Their Role in the Formation of a Stable Outer Membrane J. Biol. Chem., October 9, 1998; 273(41): 26310 - 26316. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
