| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 7, 4143-4148, February 13, 1998
From the Banting and Best Department of Medical Research and
Department of Molecular and Medical Genetics, University of Toronto,
Toronto, Ontario M5G 1L6, Canada
The association of the transcriptional
antitermination protein N of bacteriophage
Involvement of boxA Nucleotides in the Formation of a
Stable Ribonucleoprotein Complex Containing the Bacteriophage
N
Protein
with Escherichia
coli RNA polymerase depends on nut site RNA
(boxA + boxB) in the nascent transcript and the host protein, NusA. This ribonucleoprotein complex can transcribe through Rho-dependent and intrinsic termination sites
located up to several hundred base pairs downstream of nut.
For antitermination to occur farther downstream, this core
antitermination complex must be stabilized by the host proteins NusB,
NusG, and ribosomal protein S10. Here, we show that the assembly of
NusB, NusG, and S10 onto the core complex involves nucleotides 2-7 of
boxA (CGCUCUUACACA) and is a fully
cooperative process that depends on the presence of all three proteins.
This assembly of NusB, NusG, and S10 also requires the
carboxyl-terminal region (amino acids 73-107) of N, which interacts
directly with RNA polymerase. NusB and S10 assemble in the absence of
NusG when boxA is altered at nucleotides 8 and 9 to
create a consensus version of boxA
(CGCUCUUUAACA). These experiments suggest that multiple
protein-protein and protein-RNA interactions are required to convert a
core antitermination complex into a complete complex.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. S. Ciampi Rho-dependent terminators and transcription termination. Microbiology, September 1, 2006; 152(Pt 9): 2515 - 2528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Greive, A. F. Lins, and P. H. von Hippel Assembly of an RNA-Protein Complex: BINDING OF NusB AND NusE (S10) PROTEINS TO boxA RNA NUCLEATES THE FORMATION OF THE ANTITERMINATION COMPLEX INVOLVED IN CONTROLLING rRNA TRANSCRIPTION IN ESCHERICHIA COLI J. Biol. Chem., October 28, 2005; 280(43): 36397 - 36408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Bonin, R. Muhlberger, G. P. Bourenkov, R. Huber, A. Bacher, G. Richter, and M. C. Wahl Structural basis for the interaction of Escherichia coli NusA with protein N of phage {lambda} PNAS, September 21, 2004; 101(38): 13762 - 13767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Kim, J.-g. Zhou, H. R. Wilson, G. Mogilnitskiy, D. L. Court, and M. E. Gottesman Phage HK022 Nun protein represses translation of phage lambda N (transcription termination/translation repression) PNAS, April 29, 2003; 100(9): 5308 - 5312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gong and C. Yanofsky Analysis of Tryptophanase Operon Expression in Vitro. ACCUMULATION OF TnaC-PEPTIDYL-tRNA IN A RELEASE FACTOR 2-DEPLETED S-30 EXTRACT PREVENTS Rho FACTOR ACTION, SIMULATING INDUCTION J. Biol. Chem., May 3, 2002; 277(19): 17095 - 17100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Kropinski Sequence of the Genome of the Temperate, Serotype-Converting, Pseudomonas aeruginosa Bacteriophage D3 J. Bacteriol., November 1, 2000; 182(21): 6066 - 6074. [Abstract] [Full Text]
|
|
|
|
|
|
R. A. Weisberg and M. E. Gottesman Processive Antitermination J. Bacteriol., January 15, 1999; 181(2): 359 - 367. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
