| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 8, 4485-4491, February 20, 1998
From the Although glucose regulates the biosynthesis of a
variety of beta cell proteins at the level of translation, the
mechanism responsible for this effect is unknown. We demonstrate that
incubation of pancreatic islets with elevated glucose levels results in
rapid and concentration-dependent phosphorylation of
PHAS-I, an inhibitor of mRNA cap-binding protein, eukaryotic
initiation factor (eIF)-4E. Our initial approach was to determine if
this effect is mediated by the metabolism of glucose and activation of
islet cell protein kinases, or whether insulin secreted from the beta
cell stimulates phosphorylation of PHAS-I via an insulin-receptor
mechanism as described for insulin-sensitive cells. In support of the
latter mechanism, inhibitors of islet cell protein kinases A and C
exert no effect on glucose-stimulated phosphorylation of PHAS-I,
whereas the phosphatidylinositol 3-kinase inhibitor, wortmannin, the
immunosuppressant, rapamycin, and theophylline, a phosphodiesterase
inhibitor, promote marked dephosphorylation of PHAS-I. In addition,
exogenous insulin and endogenous insulin secreted by the beta cell
line,
Insulin Mediates Glucose-stimulated Phosphorylation of PHAS-I by
Pancreatic Beta Cells
AN INSULIN-RECEPTOR MECHANISM FOR AUTOREGULATION OF PROTEIN
SYNTHESIS BY TRANSLATION
,,,,,,
Department of Pathology, Washington
University School of Medicine, St. Louis Missouri 63110 and
§ Department of Pharmacology, University of Virginia School
of Medicine, Charlottesville, Virginia 22908
TC6-F7, increase phosphorylation of PHAS-I, suggesting that
beta cells of the islet, in part, mediate this effect. Studies with
beta cell lines and islets indicate that amino acids are required for glucose or exogenous insulin to stimulate the phosphorylation of
PHAS-I, and amino acids alone dose-dependently stimulate
the phosphorylation of PHAS-I, which is further enhanced by insulin. Furthermore, rapamycin inhibits by ~62% the increase in total protein synthesis stimulated by high glucose concentrations. These results indicate that glucose stimulates PHAS-I phosphorylation via
insulin interacting with its own receptor on the beta cell which may
serve as an important mechanism for autoregulation of protein synthesis
by translation.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Scheuner and R. J. Kaufman The Unfolded Protein Response: A Pathway That Links Insulin Demand with {beta}-Cell Failure and Diabetes Endocr. Rev., May 1, 2008; 29(3): 317 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Gleason, D. Lu, L. A. Witters, C. B. Newgard, and M. J. Birnbaum The Role of AMPK and mTOR in Nutrient Sensing in Pancreatic beta-Cells J. Biol. Chem., April 6, 2007; 282(14): 10341 - 10351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Tremblay, A. Gagnon, A. Veilleux, A. Sorisky, and A. Marette Activation of the Mammalian Target of Rapamycin Pathway Acutely Inhibits Insulin Signaling to Akt and Glucose Transport in 3T3-L1 and Human Adipocytes Endocrinology, March 1, 2005; 146(3): 1328 - 1337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Gomez, M. L. Powell, I. C. Greenman, and T. P. Herbert Glucose-stimulated Protein Synthesis in Pancreatic {beta}-Cells Parallels an Increase in the Availability of the Translational Ternary Complex (eIF2-GTP{middle dot}Met-tRNAi) and the Dephosphorylation of eIF2{alpha} J. Biol. Chem., December 24, 2004; 279(52): 53937 - 53946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Kwon, C. A. Marshall, K. L. Pappan, M. S. Remedi, and M. L. McDaniel Signaling Elements Involved in the Metabolic Regulation of mTOR by Nutrients, Incretins, and Growth Factors in Islets Diabetes, December 1, 2004; 53(suppl_3): S225 - S232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Hinke, K. Hellemans, and F. C. Schuit Plasticity of the {beta} cell insulin secretory competence: preparing the pancreatic {beta} cell for the next meal J. Physiol., July 15, 2004; 558(2): 369 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Andreozzi, C. D'Alessandris, M. Federici, E. Laratta, S. Del Guerra, S. Del Prato, P. Marchetti, R. Lauro, F. Perticone, and G. Sesti Activation of the Hexosamine Pathway Leads to Phosphorylation of Insulin Receptor Substrate-1 on Ser307 and Ser612 and Impairs the Phosphatidylinositol 3-Kinase/Akt/Mammalian Target of Rapamycin Insulin Biosynthetic Pathway in RIN Pancreatic {beta}-Cells Endocrinology, June 1, 2004; 145(6): 2845 - 2857. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Hawkins and L. J. Fischer Inhibition of Insulin Synthesis by Cyproheptadine: Effects on Translation Toxicol. Sci., June 1, 2004; 79(2): 258 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Otani, R. N. Kulkarni, A. C. Baldwin, J. Krutzfeldt, K. Ueki, M. Stoffel, C. R. Kahn, and K. S. Polonsky Reduced {beta}-cell mass and altered glucose sensing impair insulin-secretory function in {beta}IRKO mice Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E41 - E49. [Abstract] [Full Text]
|
|
|
|
|
|
M. I. Borelli, F. Francini, and J. J. Gagliardino Autocrine regulation of glucose metabolism in pancreatic islets Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E111 - E115. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Yonezawa Identification of TOR-interacting Proteins Mol. Interv., June 1, 2003; 3(4): 189 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yan, A. C. Nairn, H. C. Palfrey, and M. J. Brady Glucose Regulates EF-2 Phosphorylation and Protein Translation by a Protein Phosphatase-2A-dependent Mechanism in INS-1-derived 832/13 Cells J. Biol. Chem., May 9, 2003; 278(20): 18177 - 18183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Borge Jr. and B. A. Wolf Insulin Receptor Substrate 1 Regulation of Sarco-endoplasmic Reticulum Calcium ATPase 3 in Insulin-secreting beta -Cells J. Biol. Chem., March 21, 2003; 278(13): 11359 - 11368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, S. I. Taylor, S.-L. Tan, and N. Sonenberg When Translation Meets Metabolism: Multiple Links to Diabetes Endocr. Rev., February 1, 2003; 24(1): 91 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Schuit, D. Flamez, A. De Vos, and D. Pipeleers Glucose-Regulated Gene Expression Maintaining the Glucose-Responsive State of {beta}-Cells Diabetes, December 1, 2002; 51(90003): S326 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Borge, J. Moibi, S. R. Greene, M. Trucco, R. A. Young, Z. Gao, and B. A. Wolf Insulin Receptor Signaling and Sarco/Endoplasmic Reticulum Calcium ATPase in {beta}-Cells Diabetes, December 1, 2002; 51(90003): S427 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Johnson and S. Misler Nicotinic acid-adenine dinucleotide phosphate-sensitive calcium stores initiate insulin signaling in human beta cells PNAS, October 29, 2002; 99(22): 14566 - 14571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Galbiati, L. Polastri, S. Gregori, M. Freschi, M. Casorati, U. Cavallaro, P. Fiorina, F. Bertuzzi, A. Zerbi, G. Pozza, et al. Antitumorigenic and Antiinsulinogenic Effects of Calcitriol on Insulinoma Cells and Solid {beta}-Cell Tumors Endocrinology, October 1, 2002; 143(10): 4018 - 4030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. McDaniel, C. A. Marshall, K. L. Pappan, and G. Kwon Metabolic and Autocrine Regulation of the Mammalian Target of Rapamycin by Pancreatic {beta}-Cells Diabetes, October 1, 2002; 51(10): 2877 - 2885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lobner, H. Steinbrenner, G. A. Roberts, Z. Ling, G.-C. Huang, S. Piquer, D. G. Pipeleers, J. Seissler, and M. R. Christie Different Regulated Expression of the Tyrosine Phosphatase-Like Proteins IA-2 and Phogrin by Glucose and Insulin in Pancreatic Islets: Relationship to Development of Insulin Secretory Responses in Early Life Diabetes, October 1, 2002; 51(10): 2982 - 2988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Leibowitz, G. Uckaya, A. I. Oprescu, E. Cerasi, D. J. Gross, and N. Kaiser Glucose-Regulated Proinsulin Gene Expression Is Required for Adequate Insulin Production during Chronic Glucose Exposure Endocrinology, September 1, 2002; 143(9): 3214 - 3220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Lynch, B. J. Patson, J. Anthony, A. Vaval, L. S. Jefferson, and T. C. Vary Leucine is a direct-acting nutrient signal that regulates protein synthesis in adipose tissue Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E503 - E513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Shen, D. Mallon, D. W. Boyle, and E. A. Liechty IGF-I and insulin regulate eIF4F formation by different mechanisms in muscle and liver in the ovine fetus Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E593 - E603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Roper, W.-j. Qian, B. B. Zhang, R. N. Kulkarni, C. R. Kahn, and R. T. Kennedy Effect of the Insulin Mimetic L-783,281 on Intracellular [Ca2+] and Insulin Secretion From Pancreatic {beta}-Cells Diabetes, February 1, 2002; 51(90001): S43 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Greiwe, G. Kwon, M. L. McDaniel, and C. F. Semenkovich Leucine and insulin activate p70 S6 kinase through different pathways in human skeletal muscle Am J Physiol Endocrinol Metab, September 1, 2001; 281(3): E466 - E471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-C. Gingras, B. Raught, and N. Sonenberg Regulation of translation initiation by FRAP/mTOR Genes & Dev., April 1, 2001; 15(7): 807 - 826. [Full Text]
|
|
|
|
|
|
G. Xu, G. Kwon, W. S. Cruz, C. A. Marshall, and M. L. McDaniel Metabolic Regulation by Leucine of Translation Initiation Through the mTOR-Signaling Pathway by Pancreatic {beta}-Cells Diabetes, February 1, 2001; 50(2): 353 - 360. [Abstract] [Full Text]
|
|
|
|
|
|
H. Zhang, J. P. Stallock, J. C. Ng, C. Reinhard, and T. P. Neufeld Regulation of cellular growth by the Drosophila target of rapamycin dTOR Genes & Dev., November 1, 2000; 14(21): 2712 - 2724. [Abstract] [Full Text]
|
|
|
|
|
|
O. J. Shah, D. A. Antonetti, S. R. Kimball, and L. S. Jefferson Leucine, Glutamine, and Tyrosine Reciprocally Modulate the Translation Initiation Factors eIF4F and eIF2B in Perfused Rat Liver J. Biol. Chem., December 17, 1999; 274(51): 36168 - 36175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kwon, G. Xu, C. A. Marshall, and M. L. McDaniel Tumor Necrosis Factor alpha -induced Pancreatic beta -Cell Insulin Resistance Is Mediated by Nitric Oxide and Prevented by 15-Deoxy-Delta 12,14-prostaglandin J2 and Aminoguanidine. A ROLE FOR PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR gamma ACTIVATION AND iNOS EXPRESSION J. Biol. Chem., June 25, 1999; 274(26): 18702 - 18708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. G. Xu, Z.-y. Gao, P. D. Borge Jr., and B. A. Wolf Insulin Receptor Substrate 1-induced Inhibition of Endoplasmic Reticulum Ca2+ Uptake in beta -Cells. AUTOCRINE REGULATION OF INTRACELLULAR Ca2+ HOMEOSTASIS AND INSULIN SECRETION J. Biol. Chem., June 18, 1999; 274(25): 18067 - 18074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Aspinwall, J. R. T. Lakey, and R. T. Kennedy Insulin-stimulated Insulin Secretion in Single Pancreatic Beta Cells J. Biol. Chem., March 5, 1999; 274(10): 6360 - 6365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shigemitsu, Y. Tsujishita, K. Hara, M. Nanahoshi, J. Avruch, and K. Yonezawa Regulation of Translational Effectors by Amino Acid and Mammalian Target of Rapamycin Signaling Pathways. POSSIBLE INVOLVEMENT OF AUTOPHAGY IN CULTURED HEPATOMA CELLS J. Biol. Chem., January 8, 1999; 274(2): 1058 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Angenstein, W. T. Greenough, and I. J. Weiler Metabotropic glutamate receptor-initiated translocation of protein kinase p90rsk to polyribosomes: A possible factor regulating synaptic protein synthesis PNAS, December 8, 1998; 95(25): 15078 - 15083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Fox, P. T. Pham, S. R. Kimball, L. S. Jefferson, and C. J. Lynch Amino acid effects on translational repressor 4E-BP1 are mediated primarily by L-leucine in isolated adipocytes Am J Physiol Cell Physiol, November 1, 1998; 275(5): C1232 - C1238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Xu, G. Kwon, C. A. Marshall, T.-A. Lin, J. C. Lawrence Jr., and M. L. McDaniel Branched-chain Amino Acids Are Essential in the Regulation of PHAS-I and p70 S6 Kinase by Pancreatic beta -Cells. A POSSIBLE ROLE IN PROTEIN TRANSLATION AND MITOGENIC SIGNALING J. Biol. Chem., October 23, 1998; 273(43): 28178 - 28184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Leibiger, K. Wahlander, P.-O. Berggren, and I. B. Leibiger Glucose-stimulated Insulin Biosynthesis Depends on Insulin-stimulated Insulin Gene Transcription J. Biol. Chem., September 22, 2000; 275(39): 30153 - 30156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Wicksteed, T. P. Herbert, C. Alarcon, M. K. Lingohr, L. G. Moss, and C. J. Rhodes Cooperativity between the Preproinsulin mRNA Untranslated Regions Is Necessary for Glucose-stimulated Translation J. Biol. Chem., June 15, 2001; 276(25): 22553 - 22558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Tremblay and A. Marette Amino Acid and Insulin Signaling via the mTOR/p70 S6 Kinase Pathway. A NEGATIVE FEEDBACK MECHANISM LEADING TO INSULIN RESISTANCE IN SKELETAL MUSCLE CELLS J. Biol. Chem., October 5, 2001; 276(41): 38052 - 38060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Aspinwall, W.-J. Qian, M. G. Roper, R. N. Kulkarni, C. R. Kahn, and R. T. Kennedy Roles of Insulin Receptor Substrate-1, Phosphatidylinositol 3-Kinase, and Release of Intracellular Ca2+ Stores in Insulin-stimulated Insulin Secretion in beta -Cells J. Biol. Chem., July 14, 2000; 275(29): 22331 - 22338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Webb, M. S. Akbar, C. Zhao, and D. F. Steiner Expression profiling of pancreatic beta cells: Glucose regulation of secretory and metabolic pathway genes PNAS, May 23, 2000; 97(11): 5773 - 5778. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
