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J Biol Chem, Vol. 273, Issue 8, 4539-4546, February 20, 1998

Molecular Cloning of a T Cell-specific Adapter Protein (TSAd) Containing an Src Homology (SH) 2 Domain and Putative SH3 and Phosphotyrosine Binding Sites

Anne SpurklandDagger , Jan E. BrinchmannDagger , Gunnar MarkussenDagger , Florence Pedeutour, Else Munthepar , Tor Lea**, Frode VartdalDagger , and Hans-Christian Aasheimpar

From the Dagger  Institute of Transplantation Immunology, National Hospital, 0027 Oslo, Norway,  URA CNRS, Faculté de Medicine, Avenue de Valombrose, Nice, France, the ** Institute of Immunology and Rheumatology, National Hospital, 0027 Oslo, Norway, and the par  Department of Immunology, Radiumhospitalet, 0310 Oslo, Norway

Adapter proteins link catalytic signaling proteins to cell surface receptors or downstream effector proteins. In this paper, we present the cDNA sequence F2771, isolated from an activated CD8+ T cell cDNA library. The F2771 cDNA encodes a novel putative adapter protein. The predicted amino acid sequence includes an SH2 domain as well as putative SH3 and phosphotyrosine binding interaction motifs, but lacks any known catalytic domains. The expression of the gene is limited to tissues of the immune system and, in particular, activated T cells. The protein expressed by F2771 cDNA in transfected COS cells is localized in the cytoplasm. A polyclonal antiserum raised against an F2771-encoded peptide reacts with a tyrosine-phosphorylated 52-kDa protein expressed in phytohemagglutinin-stimulated peripheral blood mononuclear cells. The gene is localized to chromosome 1q21, a region often found to be aberrant in lymphomas. The T cell-specific expression and the rapid induction of mRNA expression upon receptor binding, as well as the lack of catalytic domains in the presence of protein interaction domains, indicate that the F2771 gene encodes a novel T cell-specific adapter protein (TSAd) involved in the control of T cell activation.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.


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Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.