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J Biol Chem, Vol. 273, Issue 9, 5343-5348, February 27, 1998

Differential Interactions of the Growth Factor Receptor-bound Protein 2 N-SH3 Domain with Son of Sevenless and Dynamin
POTENTIAL ROLE IN THE Ras-DEPENDENT SIGNALING PATHWAY

Michel Vidal, José-Luis Montiel, Didier Cussac, Fabrice Cornille, Marc Duchesne, Fabienne Parker, Bruno Tocqué, Bernard-Pierre Roques, and Christiane Garbay

From the Département de Pharmacochimie Moléculaire et Structurale, U266 INSERM-URA D1500 CNRS, Université René Descartes-UFR des Sciences Pharmaceutiques et Biologiques 4, Avenue de l'Observatoire, 75270 Paris Cedex 06 and  Rhône Poulenc Rorer, Centre de Vitry-Alfortville, 13 Quai Jules Guesde, 94403 Vitry/Seine Cedex, France

In this paper, we show that the 36-45 surface-exposed sequence WYKAELNGKD of growth factor receptor-bound protein 2 (Grb2) N-SH3 domain inhibits the interaction between Grb2 and a 97-kDa protein identified as dynamin. Moreover, the peptide GPPPQVPSRPNR from dynamin also blocks the binding of dynamin to the proline-rich recognition platform of Grb2. Mutations in the 36-45 motif show that Glu-40 is critical for dynamin recognition. These observations were confirmed by immunoprecipitation experiments, carried out using ER 22 cells. It was also observed that the proline-rich peptide from dynamin was unable to dissociate the Grb2·Sos complex, whereas the proline-rich peptide from Son of sevenless (Sos) inhibited Grb2·dynamin interaction. A time-dependent stimulation of epidermal growth factor receptor overexpressing clone 22 (ER 22) cells by epidermal growth factor resulted in an immediate increase of the Grb2·Sos complex and a concomitant decrease in Grb2·dynamin. This suggests that the recruitment of Grb2·Sos to the membrane, triggered by epidermal growth factor stimulation, activates the Ras-dependent signaling and simultaneously enhances free dynamin levels, leading to both receptor internalization and endocytotic processes.

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