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J Biol Chem, Vol. 274, Issue 10, 6602-6609, March 5, 1999
From the Centre de Recherche en Infectiologie, Centre Hospitalier
de l'Université Laval, CHUQ et Faculté de Médecine
de l'Université Laval, Québec G1V 4G2, Canada
Leishmania cycles between the insect
vector and its mammalian host undergoing several important changes
mediated by the stage-specific expression of a number of genes. Using a
genomic differential screening approach, we isolated differentially
expressed cosmid clones carrying several copies of the mini-exon gene.
We report that the spliced leader (SL) RNA, essential for the
maturation of all pre-mRNAs by trans-splicing, is developmentally
regulated in Leishmania donovani amastigotes and that this
regulation is rapidly induced upon parasite growth under acidic
conditions. Stage-specific regulation of the SL RNA is associated with
the expression of a larger ~170-nucleotide transcript that bears an additional 15-nucleotide sequence at its 3'-end and is polyadenylated in contrast to the mature SL RNA. The poly(A)+ SL RNA
represents 12-16% of the total SL transcript synthesized in
amastigotes and is 2.5-3-fold more stable than the
poly(A)
Developmental Regulation of Spliced Leader RNA Gene in
Leishmania donovani Amastigotes Is Mediated by Specific
Polyadenylation
transcript. The poly(A)+ SL
transcript is synthesized specifically from one class of the genomic
mini-exon copies. Polyadenylation of the SL RNA may control the levels
of the SL mature transcript under amastigote growth and may represent
an additional step in the gene regulation process during parasite differentiation.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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