| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 11, 6875-6881, March 12, 1999
From the Clusterin is a highly conserved protein which is
expressed at increased levels by many cell types in response to a broad
variety of stress conditions. A genuine physiological function for
clusterin has not yet been established. The results presented here
demonstrate for the first time that clusterin has chaperone-like
activity. At physiological concentrations, clusterin potently protected glutathione S-transferase and catalase from heat-induced
precipitation and
Clusterin Has Chaperone-like Activity Similar to That of Small
Heat Shock Proteins
,
Department of Biological Sciences and
§ Department of Chemistry, The University of Wollongong,
Northfields Avenue, Wollongong, New South Wales 2522, Australia and the
¶ Department of Biochemistry, The University of Sydney, Sydney,
New South Wales 2006, Australia
-lactalbumin and bovine serum albumin from
precipitation induced by reduction with dithiothreitol. Enzyme-linked
immunosorbent assay data showed that clusterin bound preferentially to
heat-stressed glutathione S-transferase and to
dithiothreitol-treated bovine serum albumin and -lactalbumin.
Size exclusion chromatography and SDS-polyacrylamide gel
electrophoresis analyses showed that clusterin formed high molecular
weight complexes (HMW) with all four proteins tested. Small heat shock
proteins (sHSP) also act in this way to prevent protein precipitation
and protect cells from heat and other stresses. The stoichiometric
subunit molar ratios of clusterin:stressed protein during formation of
HMW complexes (which for the four proteins tested ranged from 1.0:1.3
to 1.0:11) is less than the reported ratios for sHSP-mediated formation
of HMW complexes (1.0:1.0 or greater), indicating that clusterin is a
very efficient chaperone. Our results suggest that clusterin may play a
sHSP-like role in cytoprotection.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  U. V. Jurkunas, M. S. Bitar, I. Rawe, D. L. Harris, K. Colby, and N. C. Joyce Increased Clusterin Expression in Fuchs' Endothelial Dystrophy Invest. Ophthalmol. Vis. Sci., July 1, 2008; 49(7): 2946 - 2955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. N. Chi, L. L. Siu, H. Hirte, S. J. Hotte, J. Knox, C. Kollmansberger, M. Gleave, E. Guns, J. Powers, W. Walsh, et al. A Phase I Study of OGX-011, a 2'-Methoxyethyl Phosphorothioate Antisense to Clusterin, in Combination with Docetaxel in Patients with Advanced Cancer Clin. Cancer Res., February 1, 2008; 14(3): 833 - 839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Janig, M. Haslbeck, A. Aigelsreiter, N. Braun, D. Unterthor, P. Wolf, N. M. Khaskhely, J. Buchner, H. Denk, and K. Zatloukal Clusterin Associates with Altered Elastic Fibers in Human Photoaged Skin and Prevents Elastin from Ultraviolet-Induced Aggregation in Vitro Am. J. Pathol., November 1, 2007; 171(5): 1474 - 1482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J Yerbury, S. Poon, S. Meehan, B. Thompson, J. R. Kumita, C. M. Dobson, and M. R. Wilson The extracellular chaperone clusterin influences amyloid formation and toxicity by interacting with prefibrillar structures FASEB J, August 1, 2007; 21(10): 2312 - 2322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ovodenko, A. Rostagno, T. A. Neubert, V. Shetty, S. Thomas, A. Yang, J. Liebmann, J. Ghiso, and R. Ritch Proteomic Analysis of Exfoliation Deposits Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1447 - 1457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ishii, Y. Sakai, and A. Nakamura Molecular Pathological Evaluation of Clusterin in a Rat Model of Unilateral Ureteral Obstruction as a Possible Biomarker of Nephrotoxicity Toxicol Pathol, April 1, 2007; 35(3): 376 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Rodriguez-Pineiro, M. P. de la Cadena, A. Lopez-Saco, and F. J. Rodriguez-Berrocal Differential Expression of Serum Clusterin Isoforms in Colorectal Cancer Mol. Cell. Proteomics, September 1, 2006; 5(9): 1647 - 1657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Carnevali, F. Luppi, D. D'Arca, A. Caporali, M. P. Ruggieri, M. V. Vettori, A. Caglieri, S. Astancolle, F. Panico, P. Davalli, et al. Clusterin Decreases Oxidative Stress in Lung Fibroblasts Exposed to Cigarette Smoke Am. J. Respir. Crit. Care Med., August 15, 2006; 174(4): 393 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zenkel, F. E. Kruse, A. G. Junemann, G. O. H. Naumann, and U. Schlotzer-Schrehardt Clusterin Deficiency in Eyes with Pseudoexfoliation Syndrome May Be Implicated in the Aggregation and Deposition of Pseudoexfoliative Material Invest. Ophthalmol. Vis. Sci., May 1, 2006; 47(5): 1982 - 1990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. So, S. Sinnemann, D. Huntsman, L. Fazli, and M. Gleave Knockdown of the cytoprotective chaperone, clusterin, chemosensitizes human breast cancer cells both in vitro and in vivo Mol. Cancer Ther., December 1, 2005; 4(12): 1837 - 1849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. J. Krijnen, S. A. G. M. Cillessen, R. Manoe, A. Muller, C. A. Visser, C. J. L. M. Meijer, R. J. P. Musters, C. E. Hack, L. A. Aarden, and H. W. M. Niessen Clusterin: a protective mediator for ischemic cardiomyocytes? Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2193 - H2202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. N. Chi, E. Eisenhauer, L. Fazli, E. C. Jones, S. L. Goldenberg, J. Powers, D. Tu, and M. E. Gleave A Phase I Pharmacokinetic and Pharmacodynamic Study of OGX-011, a 2'-Methoxyethyl Antisense Oligonucleotide to Clusterin, in Patients With Localized Prostate Cancer J Natl Cancer Inst, September 7, 2005; 97(17): 1287 - 1296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Debacq-Chainiaux, C. Borlon, T. Pascal, V. Royer, F. Eliaers, N. Ninane, G. Carrard, B. Friguet, F. de Longueville, S. Boffe, et al. Repeated exposure of human skin fibroblasts to UVB at subcytotoxic level triggers premature senescence through the TGF-{beta}1 signaling pathway J. Cell Sci., February 15, 2005; 118(4): 743 - 758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Araki, S Israel, K S Leskov, T L Criswell, M Beman, D Y Klokov, L Sampalth, K E Reinicke, E Cataldo, L D Mayo, et al. Clusterin proteins: stress-inducible polypeptides with proposed functions in multiple organ dysfunction Br. J. Radiol., January 1, 2005; Supplement_27(1): 106 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Scaltriti, A. Santamaria, R. Paciucci, and S. Bettuzzi Intracellular Clusterin Induces G2-M Phase Arrest and Cell Death in PC-3 Prostate Cancer Cells1 Cancer Res., September 1, 2004; 64(17): 6174 - 6182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Bayon, M. A. Ortiz, F. J. Lopez-Hernandez, P. H. Howe, and F. J. Piedrafita The Retinoid Antagonist MX781 Induces Clusterin Expression in Prostate Cancer Cells via Heat Shock Factor-1 and Activator Protein-1 Transcription Factors Cancer Res., August 15, 2004; 64(16): 5905 - 5912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Debure, J.-L. Vayssiere, V. Rincheval, F. Loison, Y. Le Drean, and D. Michel Intracellular clusterin causes juxtanuclear aggregate formation and mitochondrial alteration J. Cell Sci., August 1, 2003; 116(15): 3109 - 3121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Leskov, D. Y. Klokov, J. Li, T. J. Kinsella, and D. A. Boothman Synthesis and Functional Analyses of Nuclear Clusterin, a Cell Death Protein J. Biol. Chem., March 21, 2003; 278(13): 11590 - 11600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Poon, M. S. Rybchyn, S. B. Easterbrook-Smith, J. A. Carver, G. J. Pankhurst, and M. R. Wilson Mildly Acidic pH Activates the Extracellular Molecular Chaperone Clusterin J. Biol. Chem., October 11, 2002; 277(42): 39532 - 39540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Zellweger, K. Chi, H. Miyake, H. Adomat, S. Kiyama, K. Skov, and M. E. Gleave Enhanced Radiation Sensitivity in Prostate Cancer by Inhibition of the Cell Survival Protein Clusterin Clin. Cancer Res., October 1, 2002; 8(10): 3276 - 3284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. DeMattos, M. A. O'dell, M. Parsadanian, J. W. Taylor, J. A. K. Harmony, K. R. Bales, S. M. Paul, B. J. Aronow, and D. M. Holtzman Clusterin promotes amyloid plaque formation and is critical for neuritic toxicity in a mouse model of Alzheimer's disease PNAS, August 6, 2002; 99(16): 10843 - 10848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Park, H. Y. Jung, T. D. Kim, J. H. Park, C.-H. Yang, and J. Kim Distinct Roles of the N-terminal-binding Domain and the C-terminal-solubilizing Domain of alpha -Synuclein, a Molecular Chaperone J. Biol. Chem., August 2, 2002; 277(32): 28512 - 28520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Bailey, B. Aronow, J. A.K. Harmony, and M. D. Griswold Heat Shock-Initiated Apoptosis Is Accelerated and Removal of Damaged Cells Is Delayed in the Testis of Clusterin/ApoJ Knock-Out Mice Biol Reprod, April 1, 2002; 66(4): 1042 - 1053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Rosenberg, R. Girton, D. Finkel, D. Chmielewski, A. Barrie III, D. P. Witte, G. Zhu, J. J. Bissler, J. A. K. Harmony, and B. J. Aronow Apolipoprotein J/Clusterin Prevents a Progressive Glomerulopathy of Aging Mol. Cell. Biol., March 15, 2002; 22(6): 1893 - 1902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Weitzel, C. Radtke, and H. J. Seitz Two thyroid hormone-mediated gene expression patterns in vivo identified by cDNA expression arrays in rat Nucleic Acids Res., December 15, 2001; 29(24): 5148 - 5155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. E. Gerritsen, R. Robles, B. Lammle, and M. Furlan Partial amino acid sequence of purified von Willebrand factor-cleaving protease Blood, September 15, 2001; 98(6): 1654 - 1661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Zellweger, H. Miyake, S. Cooper, K. Chi, B. S. Conklin, B. P. Monia, and M. E. Gleave Antitumor Activity of Antisense Clusterin Oligonucleotides Is Improved in Vitro and in Vivo by Incorporation of 2'-O-(2-Methoxy)Ethyl Chemistry J. Pharmacol. Exp. Ther., September 1, 2001; 298(3): 934 - 940. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. W. Morgan, L. Sofer, A. S. Anderson, E. L. Bernberg, J. Cui, and J. Burnside Induction of Host Gene Expression following Infection of Chicken Embryo Fibroblasts with Oncogenic Marek's Disease Virus J. Virol., January 1, 2001; 75(1): 533 - 539. [Abstract] [Full Text]
|
|
|
|
|
|
M. Redondo, E. Villar, J. Torres-Munoz, T. Tellez, M. Morell, and C. K. Petito Overexpression of Clusterin in Human Breast Carcinoma Am. J. Pathol., August 1, 2000; 157(2): 393 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wellmann, C. Thieblemont, S. Pittaluga, A. Sakai, E. S. Jaffe, P. Siebert, and M. Raffeld Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas Blood, July 15, 2000; 96(2): 398 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Urbich, M. Fritzenwanger, A. M. Zeiher, and S. Dimmeler Laminar Shear Stress Upregulates the Complement-Inhibitory Protein Clusterin : A Novel Potent Defense Mechanism Against Complement-Induced Endothelial Cell Activation Circulation, February 1, 2000; 101(4): 352 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Girton, D. P. Sundin, and M. E. Rosenberg Clusterin protects renal tubular epithelial cells from gentamicin-mediated cytotoxicity Am J Physiol Renal Physiol, April 1, 2002; 282(4): F703 - F709. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
